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The potential of somatic hybridization in crop breeding

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Summary

In recent years, the rapid development of somatic cell genetics has made possible the transfer of alien genes over wide taxonomic distances by somatic hybridization. In this review, the potential of somatic hybridization in the breeding of crops within the Brassicaceae, Fabaceae, Poaceae and Solanaceae is discussed. It is evident from these studies that many hybrids, either symmetric or asymmetric, which are fertile have the potential to be used as a bridge between the alien species and the crop. Progeny analysis of some hybrid combinations also reveals intergenomic translocations which may lead to the introgression of the alien genes. Furthermore, fusion techniques enable the resynthesis of allopolyploid crops to increase their genetic variability and to restore ploidy level and heterozygosity after breeding at reduced ploidy level in polyploid crops.

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References

  • Afonso C.L., K.R. Harkins, M.A. Thomas-Compton, A.E. Krejci & D.W. Galbraith, 1985. Selection of somatic hybrid plants in Nicotiana through fluorescence-activated cell sorting of protoplasts. Bio/Technology 3: 811–816.

    Article  Google Scholar 

  • Akagi H., M. Sakamoto, T. Negishi & T. Fujimura, 1989. construction of rice cybrid plants. Mol. Gen. Genet. 215: 501–506.

    Article  CAS  Google Scholar 

  • Austin S., E. Lojkowska, M.K. Ehlenfeldt, A. Kelman & J.P. Helgeson, 1988. Fertile interspecific somatic hybrids of Solanum: A novel source of resistance to Erwinia soft rot. Phytopath. 78: 1216–1220.

    Article  Google Scholar 

  • Austin S., J.D. Pohlman, C.R. Brown, H. Mojtahedi, G.S. Santo, D.S. Douches & J.P. Helgeson, 1993. Interspecific somatic hybridization between Solanum tuberosum L. and S. bulbocastanum Dun. as a means of transferring nematode resistance. Am. Pot. J. 70: 485–495.

    Article  Google Scholar 

  • Austin S., M. Baer, M. Ehlenfeldt, P.J. Kazmierczak & J.P. Helgeson, 1985a. Intra-specific fusions in Solanum tuberosum. Theor. Appl. Genet. 71: 172–175.

    Google Scholar 

  • Austin S., M.A. Baer & J.P. Helgeson, 1985b. Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion. Plant Sci. 39: 75–82.

    Article  Google Scholar 

  • Austin S., M.K. Ehlenfeldt, M.A. Baer & J.P. Helgeson, 1986. Somatic hybrids produced by protoplast fusion between S. tuberosum and S. brevidens: phenotypic variation under field conditions. Theor. Appl. Genet. 71: 682–690.

    Article  Google Scholar 

  • Aziz M.A., P.K. Chand, J.B. Power & M.R. Davey, 1990. Somatic hybrids between the forage legumes Lotus corniculatus L. and L. tenuis Waldst et Kit. J. Exp. Bot. 41: 471–479.

    Article  Google Scholar 

  • Babiychuk E., S. Kushnir & Y.Y. Gleba, 1992. Spontaneous extensive chromosome elimination in somatic hybrids between somatically congruent species Nicotiana tabacum L. and Atropa belladonna L. Theor. Appl. Genet. 84: 87–91.

    Article  Google Scholar 

  • Baird E., S. Cooper-Bland, R. Waugh, M. DeMaine & W. Powell, 1992. Molecular characterization of inter- and intra-specific somatic hybrids of potato using randomly amplified polymorphic DNA (RAPD) markers. Mol. Gen. Genet. 233: 469–475.

    Article  PubMed  CAS  Google Scholar 

  • Barsby T.L., J.F. Shepard, R.J. Kemble & R. Wong, 1984. Somatic hybridization in the genus Solanum: S. tuberosum and S. brevidens. Plant Cell Rep. 3: 165–167.

    Article  Google Scholar 

  • Barsby t.L., S.A. Yarrow, R.J. Kemble & I. Grant, 1987. The transfer of cytoplasmic male sterility to winter-type oilseed rape (Brassica napus L.) by protoplast fusion. Plant Sci. 53: 243–248.

    Article  Google Scholar 

  • Bates G.W. & C.A. Hasenkampf, 1985. Culture of plant somatic hybrids following electrical fusion. Theor. Appl. Genet. 70: 227–233.

    Article  Google Scholar 

  • Bates G.W., 1990. Asymmetric hybridization between Nicotiana tabacum and N. repanda by donor recipient protoplast fusion: transfer of TMV resistance. Theor. Appl. Genet. 80: 481–487.

    Article  Google Scholar 

  • Bates G.W., C.A. Hasenkampf, C.L. Contolini & W.C. Piastuch, 1987a. Asymmetric hybridization in Nicotiana by fusion of irradiated protoplasts. Theor. Appl. Genet. 74: 718–726.

    Article  Google Scholar 

  • Bates G.W., L.J. Nea & C.A. Hasenkampf, 1987b. Electrofusion and plant somatic hybridization. p. 479–496. In: A.E. Sowers (Ed). Cell Fusion. Plenum Press, New York.

    Google Scholar 

  • Bauer-Weston B., W. Keller, J. Webb & S. Gleddie, 1993. Production and characterization of asymmetric somatic hybrids between Arabidopsis thaliana and Brassica napus. Theor. Appl. Genet. 86: 150–158.

    Article  Google Scholar 

  • Belliard G., F. Vedel & G. Pelletier, 1979. Mitochondiral recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281: 401–403.

    Article  CAS  Google Scholar 

  • Benatzky R. & S.D. Tanksley, 1986. Towards a saturated linkage map in tomato based on isozyme and random cDNA sequences. Genetics 112: 887–898.

    Google Scholar 

  • Binding H., S.M. Jain, J. Finger, G. Mordhorst, R. Nehls & J. Gressel, 1982. Somatic hybridization of an atrazine resistant biotype of Solanum nigrum with Solanum tuberosum. Part 1. Clonal variation in morphology and in atrazine sensitivity. Theor. Appl. Genet. 63: 273–277.

    Article  Google Scholar 

  • Bonierbale M.W., R.L. Plaisted & S.D. Tanksley, 1988. RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120: 1095–1103.

    PubMed  Google Scholar 

  • Bonnema A.B. & M.A. O'Connell, 1992. Molecular analysis of the nuclear and organellar genotype of somatic hybrid plants between tomato (Lycopersicon esculentum) and Lycopersicon chilense. Plant Cell Rep. 10: 629–632.

    Article  CAS  Google Scholar 

  • Bonnett H.T. & K. Glimelius, 1990. Cybrids of Nicotiana tabacum and Petunia hybrida have an intergeneric mixture of chloroplasts from P. hybrida and mitochondria identical or similar to N. tabacum. Theor. Appl. Genet. 79: 550–555.

    Article  Google Scholar 

  • Butenko R.G. & A.A. Kuchko, 1980. Somatic hybridization of Solanum tuberosum L. and Solanum chacoense Bitt. by protoplast fusion. p. 293–300. In: L. Ferenczy & G.L. Farkas (Eds). Advances in Protoplast Research. Akad. Kiado, Budapest.

    Google Scholar 

  • Böttcher U.F., D. Aviv & E. Galun, 1989. Complementation between protoplasts treated with either of two metabolic inhibitors results in somatic-hybrid plants. Plant Sci. 63: 67–77.

    Article  Google Scholar 

  • Cardi T., F. D'Ambrosio, D. Consoli, K.J. Puite & K.S. Ramulu, 1993. Production of somatic hybrids between frost-tolerant Solanum commersonii and S. tuberosum: characterization of hybrid plants. Theor. Appl. Genet. 87: 193–200.

    Article  Google Scholar 

  • Carlson P.S., H.H. Smith & R.D. Dearing, 1972. Parasexual interspecific plant hybridization. Proc. Natl. Acad. Sci. USA 69: 2292–2294.

    Article  PubMed  Google Scholar 

  • Chaput M.-H., D. Sihachakr, G. Ducreux, D. Marie & N. Barghi, 1990. Somatic hybrid plants produced by electrofusion between dihaploid potatoes: BF 15 (H1), Aminca (H6) and Cardinal (H3). Plant Cell Rep. 9: 411–414.

    Article  Google Scholar 

  • Chatterjee G.S., S.R. Sikdar, S. Das & S.K. Sen, 1988. Intergeneric somatic hybrid production through protoplast fusion between Brassica juncea and Diplotaxis muralis. Theor. Appl. Genet. 76: 915–922.

    Article  Google Scholar 

  • Cocking E.C., 1960. A method for the isolation of plant protoplasts and vacuoles. Nature 187: 962–963.

    Article  Google Scholar 

  • Cordesse F., F. Grellet, A.S. Reddy & M. Delseny, 1992. Genome specificity of rDNA spacer fragments from Oryza sativa L. Theor. Appl. Genet. 83: 864–870.

    Article  CAS  Google Scholar 

  • Crisp P., 1976. Trends in the breeding and cultivation of cruciferous crops. p. 69–118. In: J.G. Vaughan, A.J. MacLeod & B.M.G. Jones (Eds). The Biology and Chemistry of the Cruciferae. Academic Press, London.

    Google Scholar 

  • Cséplö A., F. Nagy & P. Maliga, 1984. Interspecific protoplast fusion to rescue a cytoplasmic lincomycin-resistant mutation into fertile Nicotiana plumbaginifolia plants. Mol. Gen. Genet. 198: 7–11.

    Article  Google Scholar 

  • Damiani F., M. Pezzotti & S. Arcioni, 1988. Electric field-mediated fusion of protoplasts of Medicago sativa L. and Medicago arborae L. J. Plant Physiol. 132: 474–479.

    Google Scholar 

  • Daunay M.C., M.H. Chaput, D. Sihachakr, M. Allot, F. Vedel & G. Ducreux, 1993. Production and characterization of fertile somatic hybrids of eggplant (Solanum melongena L.) with Solanum aethiopicum L. Theor. Appl. Genet. 85: 841–850.

    Article  Google Scholar 

  • Daunay, M.C., R.N. Lester & H. Laterrot, 1991. The use of wild species for the genetic improvement of eggplant (Solanum melongena) and tomato (Lycopersicon esculentum Mill). p. 389–412. In: L. Hawkes, R.N. Lester, M. Nee & N. Estrada (Eds). Solanaceae III: taxonomy, chemistry and evolution. Royal Botanical Gardens of Kew and Linnean Society of London.

  • Deak M., G. Donn, A. Fehér & D. Dudits, 1988. Dominant expression of a gene amplification-related herbicide resistance in Medicago hybrids. Plant Cell Rep. 7: 158–161.

    Article  CAS  Google Scholar 

  • Debnath S.C. & G. Wenzel, 1987. Selection of somatic fusion products in potato by hybrid vigour. Potato Res. 30: 371–380.

    Article  Google Scholar 

  • Deimling S., J. Zitzlsperger & G. Wenzel, 1988. Somatic fusion for breeding of tetraploid potatoes. Plant Breed. 101: 181–189.

    Article  Google Scholar 

  • Derks F.H.M., J. Wijbrandi, M. Koornneef & C.M. Colijn-Hooymans, 1991. Organelle analysis of symmetric and asymmetric hybrids between Lycopersicon peruvianum and Lycopersicon esculentum. Theot. Appl. Genet. 81: 199–204.

    Article  Google Scholar 

  • Derks F.H.M., J.C. Hakkert, W.H.J. Verbeek & C.M. Colijn-Hooymans, 1992. Genome composition of asymmetric hybrids in relation to the phylogenetic distance between the parents nucleus-chloroplast interaction. Theor. Appl. Genet. 84: 930–940.

    Article  Google Scholar 

  • Dixelius, C. & K. Glimelius, 1995. The use of somatic hybridization for interspecific transfer of plant disease resistance. pp. 75–93. In: R. Reuveni (Ed). Novel Approaches to Integrated Pest Management (In press). CRC Press and Lewis Publishers.

  • Douglas G.C., W.A. Keller & G. Setterfield, 1981. Somatic hybridization between Nicotiana rustica and N. tabacum II. Protoplast fusion and selection and regeneration of hybrid plants. Can. J. Bot. 59: 1509–1513.

    Article  CAS  Google Scholar 

  • Dudits D., E. Maroy, T. Praznovszky, Z. Olah, J. Gyorgyey & R. Cella, 1987. Transfer of resistance traits from carrot into tobacco by asymmetric somatic hybridization: Regeneration of fertile plants. Proc. Natl. Acad. Sci. USA 84: 8434–8438.

    Article  PubMed  CAS  Google Scholar 

  • Dudits D., O. Fejer, G.Y. Hadlaczky, C.S. Koncz, G. Lazar & G. Hovrath, 1980. Intergeneric gene transfer mediated by protoplast fusion. Mol. Gen. Genet. 179: 283–288.

    Article  Google Scholar 

  • Ehlenfeldt M.K. & J.P. Helgesson, 1987. Fertility of somatic hybrids from protoplast fusions of Solanum brevidens and S. tuberosum. Theor. Appl. Genet. 73: 395–402.

    Article  Google Scholar 

  • Evans D.A., C.E. Flick & R.A. Jensen, 1981. Disease resistance: incorporation into sexually incompatible somatic hybrids of the genus Nicotiana. Science 213: 907–909.

    Article  PubMed  Google Scholar 

  • Fahleson J., I. Eriksson & K. Glimelius, 1994a. Intertribal somatic hybrids between Brassica napus and Barbarea vulgaris. Plant Cell Rep 13: 411–416.

    Article  CAS  Google Scholar 

  • Fahleson J., I. Eriksson, M. Landgren, S. Stymne & K. Glimelius, 1994b. Intertribal somatic hybrids between Brassica napus and Thlaspi perfoliatum with high content of the T. perfoliatum-specific nervonic acid. Theor. Appl. Genet. 87: 795–804.

    Article  CAS  Google Scholar 

  • Fahleson J., L. Rahlén & K. Glimelius, 1988. Analysis of plants regenerated from protoplast fusions between Brassica napus and Eruca sativa. Theor. Appl. Genet. 76: 507–512.

    Article  Google Scholar 

  • Fahleson, J., U. Lagercrantz, I. Eriksson & K. Glimelius, 1993. Genetic and molecular analysis of sexual progenies from somatic hybrids between Eruca sativa and Brassica napus. Swedish University of Agricultural Sciences, dissertation, ISBN 91-567-4684-8.

  • Famelaer I., Y.Y. Gleba, V.A. Sidorov, V.A. Kaleda, A.S. Parakonny, N.V. Boryshuk, N.N. Cherep, I. Negrutiu & M. Jacobs, 1989. Intrageneric asymmetric hybrids between Nicotiana plumbaginifolia and Nicotiana sylvestris obtained by ‘gamma-fusion’. Plant Sci. 61: 105–117.

    Article  Google Scholar 

  • Fehér A., Z. Preiszner, J. Litkey, Gy. Csanádi & D. Dudits, 1992. Characterization of chromosome instability in interspecific somatic hybrids obtained by X-ray fusion between potato (Solanum tuberosum L.) and S. brevidens Phil. Theor. Appl. Genet. 84: 880–890.

    Article  Google Scholar 

  • Fish N., A. Karp & M.G.K. Jones, 1987. Improved isolation of dihaploid Solanum tuberosum protoplasts and the production of somatic hybrids between dihaploid S. tuberosum and S. brevidens. In Vitro 23: 575–580.

    Google Scholar 

  • Fish N., A. Karp & M.G.K. Jones, 1988. Production of somatic hybrids by electrofusion in Solanum. Theor. Appl. Genet. 76: 260–266.

    Google Scholar 

  • Fluhr R., D. Aviv, M. Edelman & E. Galun, 1984. Generation of heteroplastidic Nicotiana cybrids by protoplast fusion: analysis for plastid recombinant types. Theor. Appl. Genet. 67: 491–497.

    Article  CAS  Google Scholar 

  • Forsberg J., M. Landgren & K. Glimelius, 1994. Fertile somatic hybrids between Brassica napus and Arabidopsis thaliana. Plant Sci. 95: 213–223.

    Article  Google Scholar 

  • Gavrilenko T.A., N.I. Barbakar & A.V. Pavlov, 1992. Somatic hybridization between Lycopersicon esculentum and nontuberous Solanum species of the Etuberosa series. Plant Sci. 86: 203–214.

    Article  CAS  Google Scholar 

  • Gebhardt C., E. Ritter, T. Debener, U. Schachtschabel, B. Walkmeier, H. Uhrig & F. Salamini, 1989. RFLP analysis and linkage mapping in Solanum tuberosum. Theor. Appl. Genet. 78: 65–75.

    Article  Google Scholar 

  • Gibson R.W., E. Pehu, R.D. Woods & M.G.K. Jones, 1990. Resistance to potato virus Y and potato virus X in Solanum brevidens. Ann. Appl. Biol. 116: 151–156.

    Article  Google Scholar 

  • Gibson R.W., M.G.K. Jones & N. Fish, 1988. Resistance to potato leaf roll virus and potato virus Y in somatic hybrids between dihaploid Solanum tuberosum and S. brevidens. Theor. Appl. Genet. 76: 113–117.

    Article  Google Scholar 

  • Gilmour D.M., M.R. Davey & E.C. Cocking, 1987. Isolation and culture of heterokaryons following fusion of protoplasts from sexually compatible and sexually incompatible Medicago species. Plant Sci. 53: 263–270.

    Article  Google Scholar 

  • Gilmour D.M., M.R. Davey & E.C. Cocking, 1989. Production of somatic hybrid tissues following chemical and electrical fusion of protoplasts from albino cell suspensions of Medicago sativa and M. borealis. Plant Cell Rep. 8: 29–32.

    Article  Google Scholar 

  • Gleba Y.Y. & F. Hoffmann, 1979. ‘Arabidobrassica’: Plant-genome engineering by protoplast fusion. Naturwissenschaften 66: 547–554.

    Article  CAS  Google Scholar 

  • Gleba Y.Y. & F. Hoffmann, 1980. ‘Arabidobrassica’: A novel plant obtained by protoplast fusion. Planta 149: 112–117.

    Article  CAS  Google Scholar 

  • Gleba Y.Y. & K.M. Sytnik, 1984. Protoplast fusion. Genetic engineering in higher plants. Springer Verlag, Berlin.

    Google Scholar 

  • Gleba Y.Y. & L.R. Shlumukov, 1990. Selection of somatic hybrids. p. 257–286. In: P.J. Dix (Ed). Plant Cell Line Selection: Procedures and applications. VCH, Weinheim.

    Google Scholar 

  • Gleba Y.Y., S. Hinnisdaels, V.A. Sidorov, V.A. Kaleda, A.S. Parokonny, N.V. Boryshuk, N.N. Cherep, I. Negrutiu & M. Jacobs, 1988. Intergeneric asymmetric hybrids between Nicotiana plumbaginifolia and Atropa belladonna obtained by ‘gamma-fusion’. Theor. App. Genet. 76: 760–766.

    Article  Google Scholar 

  • Gleddie S., W.A. Keller & G. Setterfield, 1986. Production and characterization of somatic hybrids between Solanum melongena L. and S. sisymbriifolium Lam. Theor. Appl. Genet. 71: 613–621.

    Article  Google Scholar 

  • Glimelius K., J. Fahleson, M. Landgren, C. Sjödin & E. Sundberg, 1991. Gene transfer via somatic hybridization in plants. Trends in Biotech. 9: 24–30.

    Article  Google Scholar 

  • Glimelius K., K. Chen & H.T. Bonnett, 1981. Somatic hybridization in Nicotiana: Segregation of organellar traits among hybrid and cybrid plants. Planta 153: 504–510.

    Article  Google Scholar 

  • Glimelius K., M. Djupsjöbacka & H. Fellner-Feldegg, 1986. Selection and enrichment of plant protoplast heterokaryons of Brassicaceae by flow sorting. Plant Sci. 45: 133–141.

    Article  CAS  Google Scholar 

  • Grosser J.W. & F.G. Gmitter, 1990. Somatic hybridization of Citrus with wild relatives for germplasm enhancement and cultivar development. HortSci. 25: 147–151.

    Google Scholar 

  • Gupta P.P., O. Schieder & M. Gupta, 1984. Intergeneric nuclear gene transfer between somatically and sexually incompatible plants through asymmetric protoplast fusion. Mol. Gen. Genet. 197: 30–35.

    Article  CAS  Google Scholar 

  • Gupta V., G. Lakshimisita, M.S. Shaila, V. Jagannathan & M.S. Lakshmikumaran, 1992. Characterization of species-specific repeated DNA sequences from B. nigra. Theor. Appl. Genet. 84: 397–402.

    Article  CAS  Google Scholar 

  • Guri A. & K.C. Sink, 1988a. Organelle composition in somatic hybrids between an atrazine resistant biotype of Solanum nigrum and Solanum melongena. Plant Sci. 58: 51–58.

    Article  CAS  Google Scholar 

  • Guri A. & K.C. Sink, 1988b. Interspecific somatic hybrid plants between eggplant (Solanum melongena) and (Solanum torvum). Theor. Appl. Genet. 76: 490–496.

    Article  CAS  Google Scholar 

  • Guri A., A. Levi & K.C. Sink, 1988. Morphological and molecular characterization of somatic hybrid plants between Lycopersicon esculentum and Solanum nigrum. Mol. Gen. Genet. 212: 191–198.

    Article  CAS  Google Scholar 

  • Hall R.D., F.A. Krens & G.J.A. Roewendal, 1992. DNA radiation damage and asymmetric somatic hybridization: Is UV a potential substitute or supplement to ionising radiation in fusion experiments? Physiol Plant 85: 319–324.

    Article  CAS  Google Scholar 

  • Hammatt N., A. Lister, B. Jones, E.C. Cocking & M.R. Davey, 1992. Shoot formation from somatic hybrid callus between soybean and a perennial wild relative. Plant Sci. 85: 215–222.

    Article  CAS  Google Scholar 

  • Hammatt N., A. Lister, N.W. Blackhall, J. Gartland, T.K. Ghose, D.M. Gilmour, J.B. Power, M.R. Davey & E.C. Cocking, 1990. Selection of plant heterokaryons from diverse origins by flow cytometry. Protoplasma 154: 34–44.

    Article  Google Scholar 

  • Handley L.W., R.L. Nickels, M.W. Cameron, P.P. Moore & K.C. Sink, 1986. Somatic hybrid plants between Lycopersicon esculentum and Solanum lycopersocoides. Theor. Appl. Genet. 71: 691–697.

    Article  Google Scholar 

  • Hayashi Y., J. Kyozuka & K. Shimamoto, 1988. Hybrids of rice (Oryza sativa L.) and wild Oryza species obtained by cell fusion. Mol. Gen. Genet. 214: 6–10.

    Article  Google Scholar 

  • Hein T., T. Przewozny & O. Schieder, 1983. Culture and selection of somatic hybrids using an auxotrophic cell line. Theor. Appl. Genet. 64: 119–122.

    Article  Google Scholar 

  • Helgeson J.P., G.J. Hunt, G.T. Haberlach & S. Austin, 1986. Somatic hybrids between Solanum brevidens and Solanum tuberosum: Expression of a late blight resistance gene and potato leaf roll resistance. Plant Cell Rep. 3: 212–214.

    Article  Google Scholar 

  • Helgeson J.P., G.T. Haberlach & M.K. Ehlenfeldt, 1993. Sexual progeny of somatic hybrids between potato and Solanum brevidens: Potential for use in breeding programs. Am. Potato. J. 70: 437–452.

    Article  Google Scholar 

  • Hille J., M. Koomneef, M.S. Ramanna & P. Zabel, 1989. Tomato: a crop species amenable to improvement by cellular and molecular methods. Euphytica 42: 1–23.

    Article  CAS  Google Scholar 

  • Imamura J., M.W. Saul & I. Potrykus, 1987. X-ray irradiation promoted asymmetric somatic hybridisation and molecular analysis of the products. Theor. Appl. Genet. 74: 445–450.

    Article  CAS  Google Scholar 

  • Itoh K., M. Iwabuchi & K. Shimamoto, 1991. In situ hybridization with species-specific DNA probes gives evidence for asymetric nature of Brassica hybrids obtained by X-ray fusion. Theor. Appl. Genet. 81: 356–362.

    Article  CAS  Google Scholar 

  • Iwabuchi M., K.K. Itoh & K. Shimamoto, 1991. Molecular and cytological characterization of repetitive sequences in Brassica. Theor. Appl. Genet. 81: 349–355.

    Article  CAS  Google Scholar 

  • Jacobsen E., M.K. Daniel, J.E.M. Bergervoet-van Deelen, D.J. Huigen & M.S. Ramanna, 1993. The first and second backcross progeny of the intergeneric fusion hybrids of potato after crossing with potato. Theor. Appl. Genet. 88: 181–186.

    Google Scholar 

  • Jadari R., D. Sihachakr, L. Rossignol & G. Ducreux, 1992. Transfer of resistance to Verticillium dahliae Kleb. from Solanum torvum S.W. into potato (Solanum tuberosum L.) by protoplast electrofusion. Euphytica 64: 39–47.

    Google Scholar 

  • Jansen, C.E., 1993. Selectable cytoplasmic markers in tomato. Ph.D. Thesis, Free University, Amsterdam, The Netherlands.

  • Jones R.A.C., 1979. Resistance to potato leaf roll virus in Solanum brevidens. Potato Res. 22: 149–152.

    Article  Google Scholar 

  • Kameya T., H. Kanzaki, S. Toki & T. Abe, 1989. Transfer of radish (Raphanus sativus L.) chloroplasts into cabbage (Brassica oleracea L.) by protoplast fusion. Jpn. J. Genet. 64: 27–34.

    Article  Google Scholar 

  • Kameya T., N. Miyazawa & S. Toki, 1990. Production of somatic hybrids between Solanum melongena L. and S. integrifolium Poil. Japan. J. Breed. 40: 429–434.

    Google Scholar 

  • Kao K.N. & M.R. Michayluk, 1974. A method for high-frequency intergeneric fusion of plant protoplasts. Planta 115: 355–367.

    Article  CAS  Google Scholar 

  • Kao K.N., 1977. Chromosomal behaviour in somatic hybrids of soybean Nicotiana glauca. Mol. Gen. Genet. 150: 225–230.

    Article  Google Scholar 

  • Kemble R.J., T.L. Barsby, R.S.C. Wong & J.F. Shepard, 1986. Mitochondrial DNA rearrangements in somatic hybrids of Solanum tuberosum and Solanum brevidens. Theor. Appl. Genet. 72: 787–793.

    Article  CAS  Google Scholar 

  • Kihara M., K.-N. Cai, R. Ishikawa, T. Harada, N. Niizeki & K. Saito, 1992. Asymmetric somatic hybrid calli between leguminosus species of Lotus corniculatus and Glycine max and regenerated plants from the calli. Japan. J. Breed. 42: 55–64.

    Google Scholar 

  • Kinsara A., S.N. Patnaik, E.C. Cocking & J.B. Power, 1986. Somatic hybrid plants of Lycopersicon esculentum Mill. and Lycopersicon peruvianum Mill. J. Plant Physiol. 125: 225–234.

    Google Scholar 

  • Klimaszewska K. & W.A. Keller, 1988. Regeneration and characterization of somatic hybrids between brassica napus and Diplotaxis harra. Plant Sci. 58: 211–222.

    Article  Google Scholar 

  • Koop H.U. & H.G. Schweiger, 1985. Regeneration of plants after electrofusion of selected pairs of protoplasts. Eur. J. Cell. Biol. 39: 46–49.

    Google Scholar 

  • Kubo, T., 1988. Protoplast fusion in tobacco breeding. Proceedings of the seminar ‘Cell and Tissue culture in field crop improvement’ p. 49–53, Tsukuba, Japan.

  • Kuchuk, N.V., N.V. Borisyuk & Y.Y. Gleba, 1990. Isolation and analysis of somatic hybrid cell lines and plants of Medicago. Abstract. VIIIth International Congress on Plant Tissue and Cell Culture. p. 213. Amsterdam, The Netherlands.

  • Kumar A. & E.C. Cocking, 1987. Protoplast fusion: A novel approach to organelle genetics in higher plants. Amer. J. Bot. 74: 1289–1303.

    Article  Google Scholar 

  • Kumar V. & M.R. Davey, 1991. Genetic improvement of legumes using somatic cell and molecular techniques. Euphytica 55: 157–169.

    Article  CAS  Google Scholar 

  • Kumashiro T. & T. Kubo, 1986. Cytoplasm transfer of Nicotiana debneyi to N. tabacum by protoplast fusion. Jpn. J. Breed. 36: 39–48.

    Google Scholar 

  • Kyozuka J., T. Kaneda & K. Shimamoto, 1989. Production of cytoplasmic male sterile rice (Oryza sativa L.) by cell fusion. Bio/Technology 7: 1171–1174.

    Google Scholar 

  • Landgren M. & K. Glimelius, 1990. Analysis of chloroplast and mitochondrial segregation in three different combinations of somatic hybrids produced within Brassicaceae. Theor. Appl. Genet. 80: 776–784.

    Article  CAS  Google Scholar 

  • Lefrançois C., & Chupeau & J.P. Bourgin, 1993. Sexual and somatic hybridization in the genus Lycopersicon. Theor. Appl. Genet. 86: 533–546.

    Article  Google Scholar 

  • Lelivelt, C.L.C., 1993. Introduction of beet cyst nematode resistance from Sinapis alba L. and Raphanus sativus L. into Brassica napus L. (oil-seed rape) through sexual and somatic hybridization. Ph. D. Thesis, Univ. of Wageningen, The Netherlands.

  • Lelivelt C.L.C. & F.A. Krens, 1992. Transfer of resistance to the beet cyst nematode (Heterodera schachtii Schm.) into the Brassica napus L. gene pool through intergeneric somatic hybridization with Raphanus sativus L. Theor. Appl. Genet. 83: 887–894.

    Article  CAS  Google Scholar 

  • Lelivelt C.L.C., E.H.M. Leunissen, H.J. Frederiks, J.P.F.G. Helsper & F.A. Krens, 1993. Transfer of resistance to the beet cyst nematode (Heterodera schachtii Schm.) from Sinapis alba L. (white mustard) to the Brassica napus L. gene pool by means of sexual and somatic hybridization. Theor. Appl. Genet. 85: 688–696.

    Article  Google Scholar 

  • Levi A., B.L. Ridley & K.C. Sink, 1988. Biased organelle transmission in somatic hybrids of Lycopersicon esculentum and Solanum lycopersicoides. Curr. Genet. 14: 177–182.

    Article  CAS  Google Scholar 

  • Li Y. & K.C. Sink, 1992. Cell type determines plastid transmission in tomato intergeneric somatic hybrids. Curr. Gen. 22: 167–171.

    Article  CAS  Google Scholar 

  • Li Y.-G., G.J. Tanner, A.C. Delves & P.J. Larkin, 1993. Asymmetric somatic hybrid plants between Medicago sativa L. (alfalfa, luceme) and Onobrychis viciifolia Scop. (sainfoin). Theor. Appl. Genet. 87: 455–463.

    Google Scholar 

  • Liu, J. & K. Glimelius, 1994. Production of somatic hybrids and cybrids between Brassica napus and B. tournefortii, Abstracts VIIIth International Congress of Plant Tissue and Cell Culture. Firenze, Italy, 12–17 June 1994, p. 98.

  • Malone R., G.V. Horváth, A. Cséplö, B. Búzás, P.J. Dix & P. Medgyesy, 1992. Impact of the stringency of cell selection on plastid segregation in protoplast fusion-derived Nicotiana regenerates. Theor. Appl. Genet. 84: 866–873.

    Article  Google Scholar 

  • Masson J., D. Lancelin, C. Bellini, M. Lecerf, P. Guerche & G. Pelletier, 1989. Selection of somatic hybrids between diploid clones of potato (Solanum tuberosum L.) transformed by direct gene transfer. Theor. Appl. Genet. 78: 153–159.

    Article  Google Scholar 

  • Mattheij W.M. & K.J. Puite, 1992. Tetraploid potato hybrids through protoplast fusions and analysis on their performance in the field. Theor. Appl. Genet. 83: 807–812.

    Google Scholar 

  • Mattheij W.M., R. Eijlander, J.R.A. Koningde & K.M. Loewes, 1992. Interspecific hybridization between the cultivated potato Solanum tuberosum L. and the wild species S. circaeifolium subsp. circaeifolium Bitter exhibiting resistance to Phytophthora infestans (Mont.) de Bary and Globodera pallida (Stone) Behrens. Theor. Appl. Genet. 83: 459–466.

    Google Scholar 

  • McCabe P.F., L.J. Dunbar, A. Guri & K.C. Sink, 1993. T-DNA-tagged chromosome 12 in donor Lycopersicon esculentum × L. pennellii is retained in asymmetric somatic hybrids with recipient Solanum lycopersicoides. Theor. Appl. Genet. 86: 377–382.

    Article  Google Scholar 

  • McLennan M.S., P. Olesen & J.B. Power, 1988. Towards the introduction of cytoplasmic male sterility (CMS) into Brassica napus through protoplast fusion. p. 187–188. In: K.J. Puite, J.J.M. Dons, J.J. Huising, A.J. Kool, M. Koorneef & F.A. Krens (Eds). Progress in Plant Protoplast Research. Kluwer Academic Publishers, London.

    Google Scholar 

  • Medgyesy P., E. Fejes & P. Maliga, 1985. Interspecific chloroplast recombination in a Nicotiana somatic hybrid. Proc. Natl. Acad. Sci. USA 82: 6960–6964.

    Article  PubMed  CAS  Google Scholar 

  • Medgyesy P., L. Menczel & P. Maliga, 1980. The use of cytoplasmic streptomycin resistance: chloroplast transfer from Nicotiana tabacum into Nicotiana sylvestris, and isolation of their somatic hybrids. Mol. Genet. 179: 693–698.

    Article  CAS  Google Scholar 

  • Melchers G., M.D. Sacristán & A.A. Holder 1978. Somatic hybrid plants of potato and tomato regenerated from fused protoplasts. Carlsberg Res. Commun. 43: 203–218.

    Article  Google Scholar 

  • Melzer J.M. & M.A. O'Connell, 1990. Molecular analysis of the extent of asymmetry in two asymmetric somatic hybrids of tomato. Theor. Appl. Genet. 79: 193–200.

    Article  CAS  Google Scholar 

  • Melzer J.M. & M.A. O'Connell, 1992. Effect of radiation dose on the production of and the extent of asymmetry in tomato asymmetric somatic hybrids. Theor. Appl. Genet. 83: 337–344.

    Article  Google Scholar 

  • Menczel L., G. Galiba, F. Nagy & P. Maliga, 1982. Effect of radiation dosage on efficiency of chloroplast transfer by protoplast fusion in Nicotiana. Genetics 100: 487–495.

    PubMed  Google Scholar 

  • Menczel L., L.S. Polsby, E. Steinback & P. Maliga, 1986. Fusion-mediated transfer of triazine resistant chloroplasts: characterization of Nicotiana tabacum cybrid plants. Mol. Gen. Genet. 205: 201–205.

    Article  CAS  Google Scholar 

  • Mendis M.H., J.B. Power & M.R. Davey, 1991. Somatic hybrids of the forage legumes Medicago sativa L. and M. falcata L. J. Exp. Bot. 42: 1565–1573.

    Article  Google Scholar 

  • Metzlaff M., W. Troebner, F. Baldauf, R. Schlegel & J. Cullum, 1986. Wheat specific repetitive DNA sequences-construction and characterization of four different genomic clones. Theor. Appl. Genet. 72: 207–210.

    Article  CAS  Google Scholar 

  • Möllers C. & G. Wenzel, 1992. Somatic hybridization of diploid potato protoplasts as a tool for potato breeding. Bot. Acta 105: 133–139.

    Google Scholar 

  • Möllers C., U. Frei & G. Wenzel, 1994. Field evaluation of tetraploid somatic potato hybrids. Theor. Appl. Genet. 88: 147–152.

    Article  Google Scholar 

  • Nagao T., 1982. Somatic hybridization by fusion of protoplasts. Jpn. J. Crop Sci. 51: 35–42.

    Google Scholar 

  • Nagata T. & I. Takebe, 1971. Plating of isolated tobacco mesophyll protoplasts on agar medium. Planta 99: 12–20.

    Article  Google Scholar 

  • Niizeki M. & K. Saito, 1989. Callus formation from protoplast fusion between leguminous species of Medicago sativa and Lotus corniculatus. Japan. J. Breed. 39: 373–377.

    CAS  Google Scholar 

  • Nyman M. & A. Wallin, 1988. Plant regeneration from strawberry (Fragaria ananassa) mesophyll protoplasts. J. Plant. Physiol. 133: 375–377.

    Google Scholar 

  • Nyman M. & A. Wallin, 1992. Improved culture techniques for strawberry (Fragaria × ananassa Duch.) protoplasts and the determination of DNA content in protoplasts-derived plants. Plant Cell Tissue Organ Cult. 30: 127–133.

    Article  CAS  Google Scholar 

  • O'Connell M.A. & M.R. Hanson, 1987. Regeneration of somatic hybrid plants formed between Lycopersicon esculentum and Lycopersicon pennellii. Theor. Appl. Genet. 75: 83–89.

    Article  Google Scholar 

  • O'Connell M.A. & M.R. Hanson, 1986. Regeneration of somatic hybrid plants formed between Lycopersicon esculentum and Solanum rickii. Theor. Appl. Genet. 72: 59–65.

    Article  Google Scholar 

  • Ochatt S.J., E.M. Patat-Ochatt, E.L. Rech, M.R. Davey & J.B. Power, 1989. Somatic hybridization of sexually incompatible top-fruit tree rootstocks, wild pear (Pyrus communis var. pyraster L.) and Colt cherry (Prunus avium × pseudocerasus). Theor. Appl. Genet. 78: 35–41.

    Article  Google Scholar 

  • Oikairinen, S. & P.H. Ryöppy, 1992. Somatic hybridization of Brassica campestris and Barbarea species. Abstract at the XIIIth EUCARPIA Congress, Angers, France 6–11 July 1992, p. 261–262.

  • Okamura M., 1988. Regeneration and evaluation of somatic hybrid plants between Solanum tuberosum and Lycopersicon pimpinellifolium. p. 213–214. In: K.J. Puite, J.J.M. Dons, H.J. Huising, A.c. Kool, M. Koorneef & F.A. Krens (Eds). Progress in Protoplast Research. Kluwer Academic Publishers, London.

    Google Scholar 

  • Ozias-Akins P., R.J. Ferl & I.K. Vasil, 1986. Somatic hybridization in the Gramineae: Pennisetum americanum (L.) K. Schum. (pearl millet) + Panicum miximum Jacq. (Guinea grass). Mol. Gen. Genet. 203: 365–370.

    Article  CAS  Google Scholar 

  • Pandeya R.S., G.C. Douglas, W.A. Keller, G. Setterfield & Z.A. Patrick, 1986. Somatic hybridization between Nicotiana rustica and N. tabacum: Development of tobacco breeding strains with disease resistance and elevated nicotine content. Z. Pflanzenzüchtg. 96: 346–352.

    Google Scholar 

  • Parokonny A.S., A.Y. Kenton, Y.Y. Gleba & M.D. Bennett, 1992. Genome reorganization in Nicotiana asymmetric somatic hybrids analysed by in situ hybridization. Plant J. 2: 863–874.

    PubMed  CAS  Google Scholar 

  • Pehu E., M. Thomas, T. Poutala, A. Karp & M.G.K. Jones, 1990a. Species-specific sequences in the genus Solanum: identification, characterization, and application to study somatic hybrids of S. brevidens and S. tuberosum. Theor. Appl. Genet. 80: 693–689.

    Article  CAS  Google Scholar 

  • Pehu E., R.W. Gibson, M.G.K. Jones & A. Karp, 1990b. Studies on the genetic bases of resistance to potato leaf roll virus, potato virus Y and potato virus X in Solanum brevidens using somatic hybrids of Solanum brevidens and Solanum tuberosum. Plant Sci. 69: 95–101.

    Article  Google Scholar 

  • Pelletier G., C. Primard, M. Ferault, F. Vedel, P. Cherit, M. Renard & R. Delourme, 1988. Use of protoplasts in plant breeding: Cytoplasmic aspects. p. 169–176. In: K.J. Puite, J.J.M. Dons, H.J. Huising, A.c. Kool, M. Koornneef, F.A. Krens (Eds). Progress in Protoplast Research. Kluwer Academic Publishers, London.

    Google Scholar 

  • Perez-Vincente R., L. Petris, M. Osusky, I. Potrykus & G. Spangenberg, 1992. Molecular and cytogenetic characterization of repetitive sequences from Lolium and Festuca: applications in the analysis of Festulolium. Theor. Appl. Genet. 84: 145–154.

    Google Scholar 

  • Perl A., D. Aviv & E. Galun, 1990. Protoplast-fusion-derived CMS potato cybrids: Potential seed-parents for hybrid, true potato seeds. J. Hered. 81: 438–442.

    Google Scholar 

  • Piastuch W.C. & G.W. Bates, 1990. Chromosomal analysis of Nicotiana asymmetric hybrids by dot blotting and in situ hybridization. Mol. Gen. Genet. 222: 97–103.

    PubMed  CAS  Google Scholar 

  • Pijnacker L.P., M.A. Ferwerda, K.J. Puite & J.G. Schaart, 1989. Chromosome elimination and mutation in tetraploid somatic hybrids of Solanum tuberosum and Solanum phureja. Plant Cell Rep. 8: 82–85.

    Article  Google Scholar 

  • Pijnacker L.P., M.A. Ferwerda, K.J. Puite & S. Roest, 1987. Elimination of Solanum phureja nucleolar chromosomes in S. tuberosum x S. phureja somatic hybrids. Theor. Appl. Genet. 73: 878–882.

    Article  Google Scholar 

  • Polgár Zs., J. Preiszner, D. Dudits & A. Fehér, 1993. Vigorous growth of fusion products allows highly efficient selection of interspecific potato somatic hybrids: molecular proofs. Plant Cell Rep. 12: 399–402.

    Article  Google Scholar 

  • Preiszner J., A. Fehér, O. Veisz, J. Sutka & D. Dudits, 1991. Characterization of morphological variation and cold resistance in interspecific somatic hybrids between potato (Solanum tuberosum L.) and S. brevidens Phil. Euphytica 57: 37–49.

    Google Scholar 

  • Primard C., F. Vedel, C. Mathieu, G. Pelletier & A.M. Chevre, 1988. Interspecific somatic hybridization between Brassica napus and Brassica hirta (Sinapis alba L.). Theor. Appl. Genet. 75: 546–552.

    Article  CAS  Google Scholar 

  • Puite K.J. & J.G. Schaart, 1993. Nuclear genomic composition of asymmetric fusion products between irradiated transgenic Solanum brevidens and S. tuberosum: limited elimination of donor chromosomes and polyploidization of the recipient genome. Theor. Appl. Genet. 86: 237–244.

    Article  CAS  Google Scholar 

  • Puite K.J., 1992. Progress in plant protoplast research. Physiol. Plant. 85: 403–410.

    Article  Google Scholar 

  • Puite K.J., P.Van Wikselaar & H. Verhoeven, 1985. Electrofusion, a simple and reproducible technique in somatic hybridization of Nicotiana plumbaginifolia mutants. Plant Cell Rep. 4: 274–276.

    Article  Google Scholar 

  • Puite K.J., S. Roest & L.P. Pijnacker, 1986. Somatic hybrid potato plants after electrofusion of diploid Solabum tuberosum and Solanum phureja. Plant Cell Rep. 5: 262–265.

    Article  CAS  Google Scholar 

  • Puite K.J., W.T. Broeke & J. Schaart, 1988. Inhibition of cell wall synthesis improves flow cytometric sorting of potato heterofusions resulting in hybrid plants. Plant Sci. 56: 61–68.

    Article  CAS  Google Scholar 

  • Pupilli F., G.M. Arcioni, G.M. Scarpa, F. Damiani & S. Arcioni, 1992. Production of interspecific somatic hybrid plants in the genus Medicago through protoplast fusion. Theor. Appl. Genet. 84: 792–797.

    Article  Google Scholar 

  • Pupilli F., S. Arcioni & F. Damiani, 1991. Protoplast fusion in the genus Medicago and isozyme analysis of parental and somatic hybrid cell lines. Plant Breed. 106: 122–131.

    Article  Google Scholar 

  • Ramulu K.S., P. Dijkhuis, H.A. Verhoeven, I. Famelaer & J. Blaas, 1992. Microprotoplastisolation, enrichment and fusion for partial genome transfer in plants. Physiol. Plant. 85: 315–318.

    Article  CAS  Google Scholar 

  • Ratushnyak Y.I., S.A. Latypov, A.M. Samoylov, N.M. Piven & Y.Y. Gleba, 1991. Introgressive hybridization of tomatoes by ‘gamma fusion’ of Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentatum Dun. protoplasts. Plant Sci. 73: 65–78.

    Article  Google Scholar 

  • Rick C.M., 1982. The potential of exotic germplasm for tomato improvement. p. 1–28. In: I.K. Vasil, W.R. Scowcroft & K.J. Frey (Eds). Plant Improvement and Somatic Cell Genetics. Academic Press, New York.

    Google Scholar 

  • Roest S. & L.J.W. Gilissen, 1989. Plant regeneration from protoplasts: a literature review. Acta Bot. Neerl. 38: 1–23.

    Google Scholar 

  • Roest S. & L.J.W. Gilissen, 1993. Regeneration from protoplasts-a supplementary literature review. Acta Bot. Neerl. 42: 1–23.

    Google Scholar 

  • Rosén B., C. Halldén & W.K. Heneen, 1988. Diploid Brassica napus somatic hybrids: Characterization of nuclear and organellar DNA. Theor. Appl. Genet. 76: 197–203.

    Article  Google Scholar 

  • Sacristán M.D., M. Gerdemann-Knörck & O. Schieder, 1989. Incorporation of hygromycin resistance in Brassica nigra and its transfer to B. napus through asymmetric protoplast fusion. Theor. Appl. Genet. 78: 194–200.

    Article  Google Scholar 

  • Sakomoto K. & T. Taguchi, 1991. Regeneration of intergeneric somatic hybrid plants between Lycopersicon esculentum and Solanum muricatum. Theor. Appl. Genet. 81: 509–513.

    Article  Google Scholar 

  • San L.H., F. Vedel, D. Sihachakr & Re'my, 1990. Morphological and molecular characterization of fertile tetraploid somatic hybrids produced by protoplast electrofusion and PEG-induced fusion between Lycopersicon esculentum Mill. and Lycopersicon peruvianum Mill. Mol. Gen. Genet. 221: 17–26.

    Article  CAS  Google Scholar 

  • Saul M.W. & I. Potrykus, 1984. Species-specific repetitive DNA used to identify interspecific somatic hybrids. Plant Cell Rep. 3: 65–67.

    Article  CAS  Google Scholar 

  • Scandalios J.G. & J.C. Sorenson, 1977. Isozymes in plant tissue culture. p. 719–730. In: J. Reinert & Y.P.S. Bajaj (Eds). Applied and Fundamental Aspects of Plant Cell Tissue and Organ Culture. Springer Verlag, Berlin.

    Google Scholar 

  • Schenk H.R. & G. Röbbelen, 1982. Somatic hybrids by fusion of protoplasts from Brassica oleracea and B. campestris. Z. Pflanzenzüchtg. 89: 278–288.

    Google Scholar 

  • Schieder O., 1982. Somatic hybridization: a new method for plant improvement. p. 239–253. In: I.K. Vasil, W.R. Scowcroft & K.J. Frey (Eds). Plant Improvement and Somatic Cell Genetics. Academic Press, New York.

    Google Scholar 

  • Schweizer G., M. Ganal, Ninnemann & V. Hemleben, 1988. Species-specific DNA sequences for identification of somatic hybrids between Lycopersicon esculentum and Solanum acaule. Theor. Appl. Genet. 75: 679–684.

    Article  CAS  Google Scholar 

  • Serraf I., D. Sihachakr, G. Ducreux, S.C. Brown, M. Allot, N. Barghi & L. Rossignol, 1991. Interspecific somatic hybridization in potato by protoplast electrofusion. Plant Sci. 76: 115–126.

    Article  Google Scholar 

  • Shepard J.F., D. Bidney, T. Barsby & R. Kemble, 1983. Genetic transfer in plants through interspecific protoplast fusion. Science 219: 683–688.

    Article  PubMed  CAS  Google Scholar 

  • Sidorov V.A., M.K. Zubko, A.A. Kuchko, I.K. Komamitsky & Y.Y. Gleba, 1987. Somatic hybridization in potato: use of gammairradiated protoplasts of Solanum pinnatisectum in genetic reconstruction. Theor. Appl. Genet. 74: 364–368.

    Article  Google Scholar 

  • Sihachakr D., R. Haicour, I. Serraf, E. Barrientos, C. Herbreteau, G. Ducreux, L. Rossignol & V. Souvannavong, 1988. Electrofusion for the production of somatic hybrid plants of Solanum melongena L. and Solanum khasianum C.B. Clark. Plant Sci. 57: 215–223.

    Article  Google Scholar 

  • Sihachakr D., R. Haicour, M.-H. Chaput, E. Barrientos, G. Ducreux & L. Rossignol, 1989. Somatic hybrid plants produced by electrofusion between Solanum melongena L. and Solanum torvum Sw. Theor. Appl. Genet. 77: 1–6.

    Article  Google Scholar 

  • Sikdar S.R., G. Chatterjee, S. Das & S.K. Sen, 1990. ‘Erussica’, the intergeneric fertile somatic hybrid developed through protoplast fusion between Eruca sativa Lam. and Brassica juncea (L.) Czem. Theor. Appl. Genet. 79: 561–567.

    Article  Google Scholar 

  • Simoens C.R., J. Gielen, M.van Montagu & D. Inzé, 1988. Characterization of highly repetitive sequences of Arabidopsis thaliana. Nucl. Acids Res. 16: 6753–6766.

    Article  PubMed  CAS  Google Scholar 

  • Sjödin C. & K. Glimelius, 1988. Screening for resistance to blackleg Phoma lingam (Tode ex Fr.) Desm. within Brassicaceae. J. Phytopathology 123: 322–332.

    Article  Google Scholar 

  • Sjödin C. & K. Glimelius, 1989a. Brassica naponigra, a somatic hybrid resistant to Phoma lingam. Theor. Appl. Genet. 77: 651–656.

    Google Scholar 

  • Sjödin C. & K. Glimelius, 1989b. Transfer of resistance against Phoma lingam to Brassica napus by asymmetric somatic hybridization combined with toxin selection. Theor. Appl. Genet. 78: 513–520.

    Article  Google Scholar 

  • Sharzhynskaya, M., J. Fahleson & K. Glimelius, 1994. Somatic hybridization in the family of Brassicaceae: production of intertribal hybrids between Brassica napus L. and Lesquerella fendleri (Gray) Wats. Abstracts VIIIth International Congress of Plant Tissue and Cell Culture, Firenze, Italy, 12–17 June 1994, p. 101.

  • Spangenberg G., M.P. Valle's, Z.Y. Wang, P. Montavon, J. Nagel & I. Potrykus, 1994. Asymmetric somatic hybridization between tall fescue (Festuca arundinacea Schreb.) and irradiated Italian ryegrass (Lolium multiflorum Lam.) protoplasts. Theor. Appl. Genet. 77: 509–519.

    Article  Google Scholar 

  • Sundberg E. & K. Glimelius, 1986. A method for production of interspecific hybrids within Brassicaceae via somatic hybridization, using resynthesis of Brassica napus as a model. Plant Sci. 43: 155–162.

    Article  Google Scholar 

  • Sundberg E. & K. Glimelius, 1991a. Production of cybrid plants within Brassicaceae by fusing protoplasts and plasmolytically induced cytoplasts. Plant Sci. 79: 205–216.

    Article  CAS  Google Scholar 

  • Sundberg E. & K. Glimelius, 1991b. Effects of parental ploidy level and genetic divergence on chromosome elimination and chloroplast segregation in somatic hybrids within Brassicaceae. Theor. Appl. Genet. 83: 81–88.

    Article  Google Scholar 

  • Sundberg E., M. Landgren & K. Glimelius, 1987. Fertility and chromosome stability in Brassica napus resynthesised by protoplast fusion. Theor. Appl. Genet. 75: 96–104.

    Article  Google Scholar 

  • Sundberg E., U. Lagercranz & K. Glimelius, 1991. Effects of cell type used for fusion on chromosome elimination and chloroplast segregation in Brassica oleracea (+) B. napus hybrids. Plant Sci. 78: 89–98.

    Article  Google Scholar 

  • Tabaeizadeh Z., R.J. Ferl & I.K. Vasil, 1986. Somatic hybridization in the Gramineae: Saccharum officinarum L. (sugarcane) and Pennisetum americanum (L.) K. Schum. (pearl millet). Proc. Natl. Acad. Sci. USA 83: 5616–5619.

    Article  PubMed  CAS  Google Scholar 

  • Taguchi T. & T. Kameya, 1986. Production of somatic hybrid plants between cabbage and chinese cabbage through protoplast fusion. Japan. J. Breed. 36: 185–189.

    Google Scholar 

  • Takamizo T., G. Spangenberg, K. Suginobu & I. Potrykus, 1991. Intergeneric somatic hybridization in Gramineae: somatic hybrid plants between tall fescue (Festuca arundinacea Schreb.) and Italian ryegrass (Lolium multiflorum Lam.) Mol. Gen. Genet. 231: 1–6.

    Article  PubMed  CAS  Google Scholar 

  • Takebe I., G. Labib & G. Melchers, 1971. Regeneration of whole plants from isolated mesophyll protoplasts of tobacco. Naturwissenschaften 58: 318–320.

    Article  Google Scholar 

  • Tan, M.-L.M.C., 1987. Somatic hybridization and cybridization in some solanaceae. PhD Thesis, Free University, Amsterdam, The Netherlands.

  • Téoulé E., 1983. Hybridation somatique entre Medicago sativa L. et Medicago falcata L.C.R. Acad. Sci. Paris 297: 13–16.

    Google Scholar 

  • Terada K., J. Kyozuka, S. Nishibayashi & K. Shimamoto, 1987a. Plantlet regeneration from somatic hybrids of rice (Oryza sativa) and barnyard grass (Echinochloa oryzicola Vasing.). Mol. Gen. Genet. 210: 39–43.

    Article  Google Scholar 

  • Terada K., Y. Yamashita, S. Nishibayashi & K. Shimamoto, 1987b. Somatic hybrids between Brassica oleracea and B. campestris: selection by the use of iodoacetamide inactivation and regeneration ability. Theor. Appl. Genet. 73: 379–384.

    Article  Google Scholar 

  • Thach N.Q., U. Frei & G. Wenzel, 1993. Somatic fusion for combining virus resistances in Solanum tuberosum L. Theor. Appl. Genet. 85: 863–867.

    Article  Google Scholar 

  • Thanh N.D., A. Páy, M.A. Smith, P. Medgyesy & L. Márton, 1988. Intertribal chloroplast transfer by protoplast fusion between Nicotiana tabacum and Salpiglossis sinuata. Mol. Gen. Genet. 213: 186–190.

    Article  Google Scholar 

  • Thomas M.R., L.B. Johnson & F.F. White, 1990. Selection of interspecific somatic hybrids of Medicago by using Agrobacterium-transformed tissues. Plant Sci. 69: 189–198.

    Article  Google Scholar 

  • Thomzik J.E. & R. Hain, 1988. Transfer and segregation of triazine tolerant chloroplasts in Brassica napus L. Theor. Appl. Genet. 76: 165–171.

    Article  Google Scholar 

  • Toriyama K. & K. Hinata, 1988. Diploid somatic-hybrid plants regenerated from rice cultivars. Theor. Appl. Genet. 76: 665–668.

    Article  Google Scholar 

  • Toriyama K., K. Hinata & T. Kameya, 1987a. Selection of a universal hybridizer in Sinapis turgida Del. and regeneration of plantlets from somatic hybrids with Brassica species. Planta 170: 308–313.

    Article  CAS  Google Scholar 

  • Toriyama K., K. Hinata & T. Kameya, 1987b. Production of somatic hybrid plants, ‘Brassicomoricandia’ through protoplast fusion between Moricandia arvensis and Brassica oleracea. Plant Sci. 48: 123–128.

    Article  Google Scholar 

  • Tsunoda S., 1980. Eco-physiology of wild and cultivated forms in Brassica and allied genera. p. 109–120. In: S. Tsunoda, K. Hinata & Gomez-Campo (Eds). Brassica Crops and Wild Allies. Jpn. Sci. Soc. Press, Tokyo.

    Google Scholar 

  • Turpin C., 1986. Attempt of male cytoplasmic sterility introduction by intergeneric fusion in cultivated tomato. Acta Hortic. 191: 377–379.

    Google Scholar 

  • Vamling K. & K. Glimelius, 1990. Regeneration of plants from protoplasts of oilseed Brassica crops. p. 385–417. In: Y.P.S. Bajaj (Ed). Biotechnology in Agriculture and Forestry, Vol 10. Springer-Verlag, Berlin.

    Google Scholar 

  • Vasil I.K. & V. Vasil, 1992. Advances in cereal protoplast research. Physiol. Plant 85: 279–283.

    Article  CAS  Google Scholar 

  • Vasil V., R. Ferl & I.K. Vasil, 1988. Somatic hybridization in the Gramineae: Triticum monococcum L. (Einkorn) + Pennisetum americanum (L.) K. Schum. (pearl millet). J. Plant Physiol. 132: 160–163.

    CAS  Google Scholar 

  • Waara S., A. Wallin & T. Eriksson, 1991. Production and analysis of intraspecific somatic hybrids of potato (Solanum tuberosum L.). Plant Sci. 75: 107–115.

    Article  Google Scholar 

  • Waara S., H. Tegelström, A. Wallin & T. Eriksson, 1989. Somatic hybridization between anther-derived dihaploid clones of potato (Solanum tuberosum L.) and the identification of hybrid plants by isozyme analysis. Theor. Appl. Genet. 77: 49–56.

    Article  CAS  Google Scholar 

  • Waara S., L.P. Pijnacker, M. Ferwerda, A. Wallin & T. Eriksson, 1992. A cytogenetic and phenotypic characterization of somatic hybrid plants obtained after fusion of two different dihaploid clones of potato (Solanum tuberosum L.) Theor. Appl. Genet. 85: 470–479.

    Article  Google Scholar 

  • Wallin A., K. Glimelius & T. Eriksson, 1974. The induction of aggregation and fusion of Daucus carota protoplasts by polyethylene glycol. Z. Pflanzenphysiol. 74: 64–80.

    CAS  Google Scholar 

  • Walton P.D. & D.C.W. Brown, 1988. Electrofusion of protoplasts and heterokaryon survival in the genus Medicago. Plant Breed. 101: 137–142.

    Article  Google Scholar 

  • Webb K.J., 1988. Recently developments in the regeneration of agronomically important crops from protoplasts. p. 27–31. In: K.J. Puite, J.J.M. Dons, H.J. Huising, A.c. Kool, M. Koornneef & F.A. Krens (Eds). Progress in Protoplast Research. Kluwer Academic Publishers, London.

    Google Scholar 

  • Wenzel G., O. Schieder, T. Przewozny, S.K. Sopory & G. Melchers, 1979. Comparison of single cell culture derived Solanum tuberosum L. plants and a model for their application in breeding programs. Theor. Appl. Genet. 55: 49–55.

    Article  Google Scholar 

  • Wijbrandi J., A. Posthuma, J.M. Kok, R. Rijken, J.G.M. Vos & M. Koornneef, 1990a. Asymmetric somatic hybrids between Lycopersicon esculentum and irradiated Lycopersicon peruvianum. 1. Cytogenetics and morphology. Theor. Appl. Genet. 80: 305–312.

    Google Scholar 

  • Wijbrandi J., A.M.A. Wolters & M. Koornneef, 1990b. Asymmetric somatic hybrids between Lycopersicon esculentum and Lycopersicon peruvianum. Theor. Appl. Genet. 80: 665–672.

    CAS  Google Scholar 

  • Wijbrandi J., P. Zabel & M. Koornneef, 1990c. Restriction fragment length polymorphism analysis of somatic hybrids between Lycopersicon esculentum and irradiated L. peruvianum: Evidence for limited donor and genome elimination and extensive chromosome rearrangements. Mol. Gen. Genet. 222: 270–277.

    Article  CAS  Google Scholar 

  • Wijbrandi J., W.Van Capelle, C.J. Hanhart, E.P.Van Loenen Martinet-Schuringa & M. Koornneef, 1990d. Selection and characterization of somatic hybrids between Lycopersicon esculentum and Lycopersicon peruvianum. Plant Sci. 70: 197–208.

    Article  Google Scholar 

  • Williams C.E., G.J. Hunt & J.P. Helgeson, 1990. Fertile somatic hybrids of Solanum species: RFLP analysis of a hybrid and its sexual progeny from crosses with potato. Theor. Appl. Genet. 80: 545–551.

    Google Scholar 

  • Wolters A.M.A., H.C.H. Schoenmakers, J.J.M.Van der Meulen-Muisers, E.Van der Knaap, F.H.M. Derks, M. Koornneef & A. Zelcer, 1991. Limited DNA elimination from the irradiated potato parent in fusion products of albino Lycopersicon esculentum and Solanum tuberosum. Theor. Appl. Genet. 83: 225–232.

    Article  Google Scholar 

  • Wright R.L., D.A. Somers & R.L. McGraw, 1987. Somatic hybridization between birdsfoot trefoil (Lotus corniculatus L.) and L. conimbricencis. Theor. Appl. Genet. 75: 51–156.

    Article  Google Scholar 

  • Xu Y.S., M. Murto, R. Dunckley, M.G.K. Jones & E. Pehu, 1993a. Production of asymmetric hybrids between Solanum tuberosum and irradiated S. brevidens. Theor. Appl. Genet. 85: 729–734.

    Google Scholar 

  • Xu Y.S., M.S. Clark & E. Pehu, 1993b. Use of RAPD markers to screen somatic hybrids between Solanum tuberosum and S. brevidens. Plant Cell Rep. 12: 107–109.

    Google Scholar 

  • Yamashita Y., R. Terada, S. Nishibayashi & K. Shimamoto, 1989. Asymmetric somatic hybrids of Brassica: partial transfer of B. campestris genome into B. oleracea by cell fusion. Theor. Appl. Genet. 77: 189–194.

    Article  Google Scholar 

  • Yang Z.-Q., T. Shikanai, K. Mori & Y. Yamada, 1989. Plant regeneration from cytoplasmic hybrids of rice (Oryza sativa L.). Theor. Appl. Genet. 77: 305–310.

    Article  CAS  Google Scholar 

  • Zelcer A., D. Aviv & E. Galun, 1978. Interspecific transfer of cytoplasmic male sterility by fusion between protoplasts of normal Nicotiana sylvestris and X-ray irradiated protoplasts of malesterile N. tabacum. Z. Pflanzenphysiol. 90: 397–407.

    Google Scholar 

  • Zhang H.B. & J. Dvorak, 1989. Isolation of repeated DNA sequences from Lophopyrum elongatum for detection of Lophopyrum chromatin in wheat genomes. Genome 33: 283–293.

    Google Scholar 

  • Ahman I., 1993. A search for resistance to insects in spring oilseed rape. IOBC/WPRS Bulletin, 16(5): 36–46.

    Google Scholar 

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  1. Uppsala Genetic Center, Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 7003, S-750 07, Uppsala, Sweden

    Sylvia Waara & Kristina Glimelius

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  1. Sylvia Waara
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Waara, S., Glimelius, K. The potential of somatic hybridization in crop breeding. Euphytica 85, 217–233 (1995). https://doi.org/10.1007/BF00023951

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