Summary
Somatic hybridization between Brassica napus and B. hirta (or Sinapis alba) is described. No cybrid plant with B. napus nucleus exhibiting cytoplasmic male sterility was recovered. Somatic hybrids were identified morphologically and, for some of them, by cytological observations. They were also characterised by Southern hybridization of nuclear rDNA. Chloroplast and mitochondrial DNA restriction analysis showed that 2 plants out of 14 have B. hirta ctDNA, one the B. napus mtDNA and the other a hybrid. Nine possess B. napus ctDNA with a hybrid mtDNA. For six of them, mtDNA patterns present novel bands, suggesting intergenomic recombination during fusion. These hybrids will be included in the breeding program.
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References
Baillargeon G (1985) In: Greuter W, Raus T (eds) Med Checklist Notulae 10, Wild de Novia, Berlin 15, pp 1:69
Bannerot H, Boulidard L, Cauderon Y, Tempe J (1974) Transfer of cytoplasmic male sterility from Raphanus sativus to Brassica oleracea. Proc Eucarpia Meeting Cruciferae, Dundee (Scotland), pp 52–54
Barsby TL, Yarrow SA, Shepard JF (1986) A rapid and efficient alternative procedure for the regeneration of plants from hypocotyl of Brassica napus. Plant Cell Rep 5:101–103
Chetrit P, Mathieu C, Vedel F, Pelletier G, Primard C (1985) Mitochondrial DNA polymorphism induced by protoplast fusion in Cruciferae. Theor Appl Genet 69:361–366
Dellaporta JL, Wood J, Hicks JB (1983) A plant minipreparation: version II. Plant Mol Biol Rep 1:19–21
Gleba Y, Kolesnik NN, Meshkene IV, Cherep NN, Parokonny AS (1984) Transmission genetics of the somatic hybridization process in Nicotiana 1. Hybrids and cybrids among the regenerates from cloned protoplast fusion products. Theor Appl Genet 69:121–128
Glimelius K, Djupsjöbacka M, Fellner-Feldegg H (1986) Selection and enrichment of plant protoplast heterokaryons of Brassiceae by flow sorting. Plant Sci 45:133–141
Harberd DJ, McArthur ED (1980) Meiotic analysis of some species and genus hybrids in the Brassiceae. In: Tsunoda S, Hinata K, Gommes-Campo C (eds) Brassica crop and wild allies. Jpn Sci Soc Press, Tokyo, pp 65–87
Labana KS (1983) Studies on in vitro interspecific hybridization in Brassica. In: Proc 6th Int Rapeseed Conf, Paris, pp 578–581
Lefort-Buson M, Dattée Y (1985) Etude de l'heterosis chez le colza oléagineux d'hiver (Brassica napus L.). Agronomie 5:101–110
Link G, Chambers SE, Thompson JA, Falk H (1981) Size and physical organization of chloroplast DNA from mustard (Sinapis alba L.). Mol Gen Genet pp 454–457
McCollum GD (1981) Induction of an alloplasmic male sterile B. oleracea by substituting cytoplasm from “early scarlet globe” radish (Raphanus sativus). Euphytica 30:855–859
McNaughton IH (1973) Resistance of Raphanobrassica to club-root disease. Nature, London 243:547–548
Moench (1802) Methodus plantas horti botanici, 1794. Suppl, pp 84
Morice J (1963) Les relations entre les espèces cultivées du genre Brassica et les possibilités d'amélioration du Colza (Brassica napus L. var. oleifera M.) au moyen de croisements interspecifiques. Ann Amelior Plant 13:221–252
Mridha MAU (1983) Virulence of different isolates of Alternaria brassicae on winter oilseed rape cultivars. In: Proc 6th Int Rapeseed Conf, Paris, pp 1025–1031
Nishi S (1980) Differentiation of Brassica crops in Asia and the breeding of “Hakuran”, a newly synthesized leafy vegetable. In: Tsunoda S, Hinata K, Gommes-Campo C (eds) Brassica crops and wild allies. Jpn Sci Soc Press, 133–150
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983) Chloroplast DNA evolution and the origin of amphiploid Brassica species. Theor Appl Genet 65:181–189
Pellan-Delourme R (1986) Etude de deux systèmes de sterilité male génocytoplasmique introduits chez le colza (Brassica napus L.) par croisements intergénériques avec Raphanus et Diplotaxis. Thèse Université de Rennes I
Pelletier G, Primard C, Vedel F, Chetrit P, Remy R, Rousselle P, Renard M (1983) Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusion. Mol Gen Genet 191:244–250
Pijnacker LP, Ferweda MA, Puite KJ, Roest S (1987) Elimination of Solanum phureja nucleolar chromosomes in S. tuberosum + S. phureja somatic hybrids. Theor Appl Genet 73:878–882
Prakash S, Hinata K (1980) Taxonomy, cytogenetics and origin of crop Brassicas, a review. Opera Bot 55:1–57
Rabenhorst F (1839) In: Flora Lusatica 1, pp184
Robertson D, Earle ED (1986) Plant regeneration from leaf protoplasts of Brassica oleracea var. italica CV Green Comet broccoli. Plant Cell Rep 5:61–64
Rothenberg M, Boeshore ML, Hanson MR, Izhar S (1985) Intergenomic recombination of mitochondrial genomes in a somatic hybrid plant. Curr Genet 9:615–618
Schenck HR, Röbbelen G (1982) Somatic hybrids by fusion of protoplasts from Brassica oleracea and B. campestris. Z Pflanzenzücht 89:278–288
Shiga T (1980) Male sterility and cytoplasmic differentiation. In: Tsunoda S, Hinata K, Gòmes-Campo C (eds) Brassica crops and wild allies. Jpn Sci Soc Press, Tokyo, pp 205–211
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Sundberg E, Glimelius K (1986) A method for production of interspecific hybrids within Brassiceae via somatic hybridization, using resynthesis of Brassica napus as a model. Plant Sci 43:155–162
Tremousaygue D, Grellet F, Delseny M, Delourme R, Renard M (1988) The large spacer of nuclear ribosomal RNA genes from radish: organisation and the use as a probe in rapeseed breeding. Theor Appl Genet 75:298–304
Vedel F, Mathieu C (1983) Physical and gene mapping of chloroplast DNA from normal and cytoplasmic male sterile (radish cytoplasm) lines of Brassica napus. Curr Genet 7:13–20
Vedel F, Mathieu C, Lebacq P, Ambard-Bretteville F, Remy R (1982) Comparative macromolecular analysis of the cytoplasms of normal and cytoplasmic male sterile Brassica napus. Theor Appl Genet 62:255–262
Vedel F, Chetrit P, Mathieu C, Pelletier G, Primard C (1986) Several different mitochondrial DNA regions are involved in intergenomic recombination in Brassica napus cybrid plants. Curr Genet 11:17–24
Verma VD, Rai B (1980) Note on induced mutagenesis for spotting out the source of resistance of Alternaria leaf spot in Indian mustard. Indian J Agric Sci 50:278–280
Zwierzykowska E (1983) Synthesis of new geneotypes within the genus Brassica by in vitro culture. In: Proc 6th Int Rapessed Conf, Paris, pp 467–471
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Primard, C., Vedel, F., Mathieu, C. et al. Interspecific somatic hybridization between Brassica napus and Brassica hirta (Sinapis alba L.). Theoret. Appl. Genetics 75, 546–552 (1988). https://doi.org/10.1007/BF00289119
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DOI: https://doi.org/10.1007/BF00289119