Summary
The karyotype of the dihaploid SVP1 line of S. tuberosum (2n=2x=24) showed two nucleolar chromosomes with differently sized satellites. The diploid SVP5 line (2n=2x=24) and tetraploid regenerants of S. phureja had larger but similar satellites. Somatic hybrids between the diploid lines of these potato species with genome combinations 4 tub + 2 ph (plants 1–3), 2 tub + 4 ph (plants 4–7) and 4 tub + 4 ph (plant 8) had lost 2 phureja nucleolar chromosomes if 4 phureja genomes were present. One phureja nucleolar chromosome of plants 1–3 and both of plants 5 and 7 had rearranged satellites. Elimination of the two nucleolar chromosomes occurred preferentially, was under genetic control, and probably took place during early callus development. NOR activity resulting in rear-rangements between NORs may have caused the elimination.
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Communicated by F. Mechelke
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Pijnacker, L.P., Ferwerda, M.A., Puite, K.J. et al. Elimination of Solanum phureja nucleolar chromosomes in S. tuberosum + S. phureja somatic hybrids. Theoret. Appl. Genetics 73, 878–882 (1987). https://doi.org/10.1007/BF00289393
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DOI: https://doi.org/10.1007/BF00289393