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Sexual and somatic hybridization in the genusLycopersicon

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Abstract

In recent years, a large number of reports have been published on the recovery of somatic hybrids in the genusLycopersicon and their potential use as a tool in plant breeding programs. Somatic hybridization as a way of enabling the incompatibility barriers which exist within the genusLycopersicon to be bypassed has attracted great interest. WildLycopersicon species harbor numerous interesting agronomic characteristics, which could be transferred to tomato by somatic hybridization. In particular, the production of asymmetric hybrids is explored as an approach to obtain the transfer of only a part of the nuclear genome of wildLycopersicon species. Considerable information is available on the fate of chloroplasts and mitochondria in fusion products inLycopersicon, and unfortunately, cybridization (transfer of chloroplasts and/or mitochondria) seems often difficult to achieve.

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References

  • Abdalla MMF, Hermsen JGT (1972) Unilateral incompatibility: hypotheses, debates and its implications for plant breeding. Euphytica 21:32–47

    Google Scholar 

  • Adams TL, Quiros CF (1985) Somatic hybridization betweenLycopersicon peruvianum andL. pennellii: regenerating ability and antibiotic resistance as selection systems. Plant Sci 40:209–219

    Google Scholar 

  • Ashley T, Pocock N (1981) A proposed model of chromosomal organization in nuclei at fertilization. Genetica 55:161–169

    Google Scholar 

  • Attathom PKS, Visessuwan R (1990) Regeneration of Seeda tomato plants from isolated protoplasts. Kasetsart J [Nat Sci Suppl] 24:6–11

    Google Scholar 

  • Barbano PP, Topoleski LD (1984) Postfertilization hybrid seed failure inLycopersicon esculentum xLycopersicon peruvianum ovules. J Am Soc Hortic Sci 109:95–100

    Google Scholar 

  • Bates GW, Hasenkampf CA, Contolini CL, Piastuch WC (1987) Asymmetric hybridization inNicotiana by fusion of irradiated protoplasts. Theor Appl Genet 74:718–726

    Google Scholar 

  • Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids ofNicotiana tabacum by protoplast fusion. Nature 281:401–402

    Google Scholar 

  • Bellini C, Chupeau MC, Gervais M, Vastra G, Chupeau Y (1990) Importance of myo-inositol, calcium, and ammonium for the viability and division of tomatoLycopersicon esculentum protoplasts. Plant Cell Tissue Organ 23:27–37

    Google Scholar 

  • Binding H (1976) Somatic hybridization experiments in Solanaceous species. Mol Gen Genet 144:171–175

    Google Scholar 

  • Bohn GW (1948) Sesquidiploid F1 hybrids ofLycopersicon esculentum andL. peruvianum. J Agric Res 77:33–53

    Google Scholar 

  • Bonnema AB, O'Connell MA (1992) Molecular analysis of the nuclear organellar genotype of somatic hybrid plants between tomato (Lycopersicon esculentum) andLycopersicon chilense. Plant Cell Rep 10:629–632

    Google Scholar 

  • Bonnema AB, Melzer JM, O'Connell MA (1991) Tomato cybrids with mitochondrial DNA fromLycopersicon pennellii. Theor Appl Genet 81:339–348

    Google Scholar 

  • Bonnema AB, Melzer JM, O'Connell MA (1992) Non-random inheritance of organellar genomes in symmetric and asymmetric somatic hybrids betweenLycopersicon esculentum andL. pennellii. Theor Appl Genet 84:435–442

    Google Scholar 

  • Chetelat RT, De Verna JW (1991) Expression of unilateral incompatibility in pollen ofLycopersicon pennellii is determined by major loci on chromosomes 1, 6 and 10. Theor Appl Genet 82:704–712

    Google Scholar 

  • Chupeau Y, Bourgin JP (1980) Les protoplastes de cellules végétales. In: Chaussat R, Bigot C (eds) La multiplication végétative des plantes supérieures. Gauthier-Villars, Paris, pp 191–221

    Google Scholar 

  • Daunay MC, Lester RN, Laterrot H (1991) The use of wild species for the genetic improvement of eggplant (Solanum melongena L.) and tomato (Lycopersicon esculentum Mill.). In: Hawkes L, Lester RN, Nee M, Estrada N (eds) Solanaceae III: Taxonomy, chemistry, evolution. Royal botanic gardens of Kew and Linnean Society of London, London, pp 389–412

    Google Scholar 

  • De Verna JW, Chetelat RT, Rick CM, Stevens MA (1987) Introduction ofSolanum lycopersicoides germplasm. In: Nevins D, Jones RA (eds) Tomato biotechnology. Alan R. Liss, New York, pp 27–36

    Google Scholar 

  • De Verna JW, Rick CM, Chetelat RT, Lanini BJ, Alpert KB (1990) Sexual hybridization ofLycopersicon esculentum and by means of a sesquidiploid bridging hybrid. Proc Natl Acad Sci USA 87:9486–9490

    Google Scholar 

  • Derks FHM, Wijbrandi J, Koornneef M, Colijn-Hooymans CM (1991) Organelle analysis of symmetric and asymmetric hybrids betweenLycopersicon peruvianum andLycopersicon esculentum. Theor Appl Genet 81:199–204

    Google Scholar 

  • Ebert PR, Anderson MA, Bernatzky R, Altschuler M, Clarke AE (1989) Genetic polymorphism of self-incompatibility in flowering plants. Cell 56:255–262

    Google Scholar 

  • Ehlenfeldt MK, Hanneman Jr RE (1992) Endosperm dosage rleationships amongLycopersicon species. Theor Appl Genet 83:367–372

    Google Scholar 

  • Famelaer I, Gleba YY, Sidorov VA, Kaleda VA, Parakonny AS, Boryshuk NV, Cherup NN, Negrutiu I, Jacobs M (1989) Intrageneric asymmetric hybrids betweenNicotiana Plumbaginifolia andNicotiana sylvestris obtained by “gamma-fusion”. Plant Sci 61:105–117

    Google Scholar 

  • Frankenberger EA, Hasegawa PM, Tigchelaar EC (1981) Influence of environment and developmental state on the shootforming capacity of tomato genotypes. Z Pflanzenphysiol 102:221–232

    Google Scholar 

  • Glas C, Kamp JC, Jongsma C, Nijkamp HJJ, Hille J (1990) The induction of lincomycin resistance inLycopersicon peruvianum andLycopersicon esculentum. Plant Sci 70:231–241

    Google Scholar 

  • Gleba YY, Parokonny A, Kotov V, Negrutiu I, Momot V (1987) Spatial separation of parental genomes in hybrids of somatic plant cells. Proc Natl Acad Sci USA 84:3709–3713

    Google Scholar 

  • Gleba YY, Hinnisdaels S, Sidorov VA, Kaleda VA, Parokonny AS, Boryshuk NV, Cherup NN, Negrutiu I, Jacobs M (1988) Intergeneric asymmetric hybrids betweenNicotiana Plumbaginifolia andAtropa belladonna obtained by “gammafusion”. Theor Appl Genet 76:760–766

    Google Scholar 

  • Gleddie S, Keller WA, Poysa V (1989) Plant regeneration from stem cortex protoplasts of a tomato hybrid. Plant Cell Rep 8:21–24

    Google Scholar 

  • Glimelius K (1985) Sexual and somatic hybridization. Hereditas 3:41–47

    Google Scholar 

  • Gradziel TM, Robinson RW (1989 a) Breakdown of self-incompatibility during pistil development inLycopersicon peruvianum by modified bud pollination. Sex Plant Reprod 2:38–42

    Google Scholar 

  • Gradziel TM, Robinson RW (1989b)Solanum lycopersicoides gene introgression to tomato,Lycopersicon esculentum, through the systematic avoidance and suppression of breeding barriers. Sex Plant Reprod 2:43–52

    Google Scholar 

  • Gradziel TM, Robinson RW (1991) Overcoming unilateral breeding barriers betweenLycopersicon peruvianum and cultivated tomato,Lycopersicon esculentum. Euphytica 54:1–9

    Google Scholar 

  • Gupta PP, Schieder O, Gupta M (1984) Intergeneric nuclear gene transfer between somatically and sexually incompatible plants through asymmetric protoplast fusion. Mol Gen Genet 197:30–35

    Google Scholar 

  • Guri A, Levi A, Sink KC (1988) Morphological and molecular characterization of somatic hybrid plants betweenLycopersicon esculentum andSolanum nigrum. Mol Gen Genet 212:191–198

    Google Scholar 

  • Guri A, Dunbar LJ, Sink KC (1991) Somatic hybridization between selectedLycopersicon andSolanum species. Plant Cell Rep 10:76–80

    Google Scholar 

  • Guy CL (1990) Cold acclimation and freezing stress tolerance: role of protein metabolism. Annu Rev Plant Physiol Plant Biol 41:187–223

    Google Scholar 

  • Han San L, Vedel F, Sihachakr D, Rémy R (1990) Morphological and molecular characterization of fertile tetraploid somatic hybrids produced by protoplast electrofusion and PEG-induced fusion betweenLycopersicon esculentum Mill, andLycopersicon peruvianum Mill. Mol Gen Genet 221:17–26

    Google Scholar 

  • Handley LW, Nickels RL, Cameron MW, Moore PP, Sink KC (1986) Somatic hybrid plants betweenLycopersicon esculentum andS. lycopersicoides. Theor Appl Genet 71:691–697

    Google Scholar 

  • Hassanpour-Estahbanati A, Demarly Y (1985 a) Plant regeneration from protoplasts ofSolanum pennellii: effect of photoperiod applied to donor plants. J Plant Physiol 121:171–174

    Google Scholar 

  • Hassanpour-Estahbanati A, Demarly Y (1985b) Plant regeneration from protoplasts ofLycopersicon chilense. Physiol Veg 24:391–396

    Google Scholar 

  • Hassanpour-Estahbanati A, Turpin C, Demarly Y (1986) Hybridizations by protoplasts fusions inSolanaceae. Acta Hortic 191:369–376

    Google Scholar 

  • Hille J, Koornneef M, Ramanna MS, Zabel P (1989) Tomato: a crop species amenable to improvement by cellular and molecular methods. Euphytica 42:1–23

    Google Scholar 

  • Hogenboom NG (1972) Breaking breeding barriers in Lycopersicon. 1. The genusLycopersicon, its breeding barriers and the importance of breaking these barriers. Euphytica 21:221–227

    Google Scholar 

  • Hogenboom NG (1973) A model for incongruity in intimate partner relationships. Euphytica 22:219–233

    Google Scholar 

  • Hosticka LP, Hanson MR (1984) Induction of plastid mutations in tomatoes by nitromethylurea. J Hered 75:242–246

    Google Scholar 

  • Imanishi S (1988) Efficient ovule culture for the hybridization ofLycopersicon esculentum andL. peruvianum var ‘glandulosum’. Jpn J Breed 38:1–9

    Google Scholar 

  • Jain SM, Shahin EA, Sun S (1988) Interspecific protoplast fusion for the transfer of atrazine resistance fromSolanum nigrum to tomato (Lycopersicon esculentum L.). Plant Cell Tissue Organ Cult 12:189–192

    Google Scholar 

  • Jansen CE, Snel EAM, Akerboom MJE, Nijkamp HJJ, Hille J (1990) Induction of streptomycin resistance in the wild tomatoLycopersicon peruvianum. Mol Gen Genet 220:260–268

    Google Scholar 

  • Jones M (1988) Fusing plant protoplasts. Tibtech 6:153–158

    Google Scholar 

  • Kalloo G (1991) Genetic improvement of tomato. In: Kalloo G (ed) Monographs on theoretical and applied genetics. Springer, pp 358

  • Kao KN, Michayluk MR (1974) A method for high frequency intergeneric fusion in plant protoplasts. Planta 115:355–367

    Google Scholar 

  • Kinsara A, Patnaik SN, Cocking EC, Power JB (1986) Somatic hybrid plants ofLycopersicon esculentum Mill, andLycopersicon peruvianum Mill. J Plant Physiol 125:225–234

    Google Scholar 

  • Koblitz H, Koblitz D (1982) Experiments on tissue culture in the genusLycopersicon Miller. Plant Cell Rep 1982:143–146

    Google Scholar 

  • Koornneef M, Hanhart CJ, Martinelli L (1987) A genetic analysis of cell culture traits in tomato. Theor Appl Genet 74:633–641

    Google Scholar 

  • Koornneef M, Bade J, Hanhart C, Horsman K, Schel J, Soppe W Verkerk R, Zabel P (1993) Characterization and mapping of a gene controlling shoot regeneration in tomato. Plant J 3:131–141

    Google Scholar 

  • Kumar A, Cocking EC (1987) Protoplast fusion: a novel approach to organelle genetics in higher plants. Am J Bot 74:1289–1303

    Google Scholar 

  • Laterrot H (1989) Intérêt et utilisation des espèces sauvages pour la création variétale. P H M — Rev Hortic 295:1–8

    Google Scholar 

  • Lee M, Phillips RL (1988) The chromosomal basis of somaclonal variation. Ann Rev Plant Physiol Plant Mol Biol 39:413–437

    Google Scholar 

  • Lefrançois C, Chupeau Y (1993) Standard conditions for plant regeneration from protoplasts of several wildLycopersicon species. J Plant Physiol (in press)

  • Lesley MM (1950) A cytological basis for sterility in tomato hybrids. J Hered 41:26–28

    Google Scholar 

  • Levi A, Ridley BL, Sink KC (1988) Biased organelle transmission in somatic hybrids ofLycopersicon esculentum andSolanum lycopersicoides. Curr Genet 14:177–182

    Google Scholar 

  • Maheswaran G, Perryman T, Williams EG (1986) Use of an interspecific hybrid in identifying a new allelic specificity generated at the self-incompatibility locus after inbreeding inLycopersicon peruvianum. Theor Appl Genet 73:236–245

    Google Scholar 

  • Martin FW (1964) The inheritance of unilateral incompatibility inLycopersicon hirsutum. Genetics 50:459–469

    Google Scholar 

  • Martin FW (1967) The genetic control of unilateral incompatibility between two tomato species. Genetics 56:391–398

    Google Scholar 

  • Martin FW (1968) The behavior ofLycopersicon incompatibility alleles in an allien genetic milieu. Genetics 60:101–109

    Google Scholar 

  • Mau SL, Williams EG, Atkinson A, Anderson MA, Cornish EC, Grego B, Simpson RJ, Kheyr-Pour A, Clarke AE (1986) Style proteins of a wild tomato (Lycopersicon peruvianum) associated with expression of self-incompatibility. Planta 169:184–191

    Google Scholar 

  • McCabe PF, Timmons AM, Dix PJ (1989) A simple procedure for the isolation of streptomycin resistant plants inSolanaceae. Mol Gen Genet 216:132–137

    Google Scholar 

  • McClure BA, Haring V, Ebert PR, Anderson MA, Simpson RJ, Sakiyama F, Clarke AE (1989) Style self-incompatibility gene products ofNicotiana alata are ribonucleases. Nature 342:955–957

    Google Scholar 

  • McClure BA, Gray JE, Anderson MA, Clarke AE (1990) Selfincompatibility inNicotiana alata involves degradation of pollen rRNA. Nature 347:757–760

    Google Scholar 

  • Medgyesy P (1989) Selection and analysis of cytoplasmic hybrids. In: Dix PJ (ed) In vitro selection of plant cells. VCH Weinheim, Basel Cambridge New York, pp 1–29

    Google Scholar 

  • Melchers G, Sacristan MD, Holder AA (1978) Somatic hybrid plants of potato and tomato regenerated from used protoplasts. Carlsberg Res Commun 43:203–218

    Google Scholar 

  • Melchers G, Mohri Y, Watanabe K, Wakabayashi S, Harada K (1992) One-step generation of cytoplasmic male sterility by fusion of mitochondrial-inactivated tomato protoplasts with nuclear-inactivatedSolanum protoplasts. Proc Natl Acad Sci USA 89:6832–6836

    Google Scholar 

  • Melzer JM, O'Connell (1990) Molecular analysis of the extent of assymetry in two assymmetric somatic hybrids of tomato. Theor Appl Genet 79:193–200

    Google Scholar 

  • Melzer JM, O'Connell MA (1992) Effect of radiation dose on the production and the extent of asymmetry in tomato asymmetric somatic hybrids. Theor Appl Genet 83:337–343

    Google Scholar 

  • Menzel MY (1962) Pachytene chromosomes of the intergeneric hybridLycopersicon esculentum xSolanum lycopersicoides. Am J Bot 49:605–615

    Google Scholar 

  • Montagno TJ, Jourdan PS, Berry SZ (1991) Plant regeneration from leaf protoplasts ofLycopersicon hirsutum f.hirsutum. Plant Cell Rep 9:680–683

    Google Scholar 

  • Moore PP, Sink KC (1988 a) Characterization of aLycopersicon Esculentum xSolanum lycopersicoides somatic hybrid lacking a glutamate oxaloacetate transaminase isozyme. Plant Cell Tissue Organ 13:39–47

    Google Scholar 

  • Moore PP, Sink KC (1988b) Molecular analysis of single copy and repetitive genes on chromosome 2 in intergeneric tomato somatic hybrid plants. Plant Mol Biol 11:139–145

    Google Scholar 

  • Morgan A, Cocking C (1982) Plant regeneration from protoplasts ofLycopersicon esculentum Mill. Z. Pflanzenphysiol 106:97–104

    Google Scholar 

  • Mutschler MA (1990) Transfer ofLycopersicon pennellii into tomato (L. Esculentum) does not create cytoplasmic male sterility. Tomato Genet Coop Rep 40:25–26

    Google Scholar 

  • Negrutiu I, Hinnisdaels S, Mouras A, Gill BS, Gharti-Chheetri GB, Gleba YY, Sidorov V, Jacobs M (1989) Somatic versus sexual hybridization: features, facts and future. Acta Bot Neerl 38:253–272

    Google Scholar 

  • Nettancourt D de, Ecochard R, Perquin MDG, Van der Drift T, Westerhof M (1971) The generation of new alleles at the incompatibility locus ofLycopersicon peruvianum Mill. Theor Appl Genet 41:120–129

    Google Scholar 

  • Nettancourt D de, Devreux M, Laneri U, Cresti M, Pacini E, Sarfatti G (1974) Genetical and ultrastructural aspects of self- and cross-incompatibility in interspecific hybrids between self-compatibleLycopersicon esculentum and self-incompatibleL. peruvianum. Theor Appl Genet 44:278–288

    Google Scholar 

  • Niedz RP, Sink KC (1988) Multifactor analysis of environmental preconditioning of tomato seedlings on protoplast culture and development. J Plant Physiol 133:385–391

    Google Scholar 

  • Niedz RP, Rutter SM, Handley LW, Sink KC (1985) Plant regeneration from leaf protoplasts of six tomato cultivars. Plant Sci 39:199–204

    Google Scholar 

  • O'Connell MA, Hanson MR (1985) Somatic hybridization betweenLycopersicon esculentum andLycopersicon pennellii. Theor Appl Genet 70:1–12

    Google Scholar 

  • O'Connell MA, Hanson MR (1986) Regeneration of somatic hybrid plants formed betweenLycopersicon esculentum andSolanum rickii. Theor Appl. Genet. 72:59–65

    Google Scholar 

  • O'Connell MA, Hanson MR (1987) Regeneration of somatic hybrid plants formed betweenLycopersicon esculentum andL. pennellii. Theor Appl Genet 75:83–89

    Google Scholar 

  • Okamura M (1987) Regeneration and evaluation of somatic hybrid plants betweenSolanum tuberosum andLycopersicon pimpinellifolium. In: Puite KJ, Dons JJM, Huizing HJ, Kool AJ, Koornneef M, Krens FA (eds) Progress in plant protoplast research. Kluwer Academic Publ Dordrecht, pp 213–214

  • Patterson BD (1988) Genes for cold resistance from wild tomatoes. Hortic Sci 23:794–947

    Google Scholar 

  • Pelletier G, Primard C, Ferault M, Vedel F, Chetrit P, Renard M, Delourme R (1988) Use of protoplasts in plant breeding: cytoplasmic aspects. Plant Cell Tissue Organ 12:173–180

    Google Scholar 

  • Perl A, Aviv D, Galun E (1991) Nuclear-organelle interaction inSolanum: interspecific cybridizations and their correlation with a plastome dendrogram. Mol Gen Genet 228:193–200

    Google Scholar 

  • Philouze J (1986) Evolution et situation variétale actuelle chez la tomate. In: A.I.C.P.C/A.C.F.E.V./B.R.G.-La diversité des plantes légumières, pp 33–42 INRA (ed)

  • Pijnacker LP, Hermelink JHM, Ferwerda MA (1986) Variability of DNA content and karyotype in cell cultures of an interdihaploidSolanum tuberosum. Plant Cell Rep 5:43–46

    Google Scholar 

  • Power JB, Cummings SE, Cocking EC (1970) Fusion of isolated plant protoplasts. Nature 225:1016–1018

    Google Scholar 

  • Poysa V (1990) The development of bridge lines for interspecific gene transfer betweenLycopersicon esculentum andL. peruvianum. Theor Appl Genet 79:187–192

    Google Scholar 

  • Quiros C, Ochoa O, Douches D (1986)L. peruvianum xL. penNellii sexual hybrids. Tomato Genet Coop Rep 36:31

    Google Scholar 

  • Ratushnyak YI, Latypov SA, Samoylov AM, Piven NM, Gleba YY (1991) Introgressive hybridization of tomatoes by “gamma-fusion” ofLycopersicon esculentum Mill, andLycopersicon peruvianum var ‘dentatum’ Dun. protoplasts. Plant Sci 73:65–78

    Google Scholar 

  • Rick CM (1951) Hybrids betweenLycopersicon esculentum Mill, andSolanum lycopersicoides Dun. Proc Natl Acad Sci USA 37:741–744

    Google Scholar 

  • Rick CM (1963) Differential zygotic lethality in a tomato species hybrid. Genetics 48:1497–1507

    Google Scholar 

  • Rick CM (1982) The potential of exotic germplasm for tomato improvement. In: Vasil IK, Scowcroft WR, Frey KJ (eds) Plant improvement and somatic cell genetics. Academic Press New York, pp 1–28

    Google Scholar 

  • Rick CM (1983) Crossability betweenL. Esculentum and a new race ofL. peruvianum. Tomato Genet Coop Rep 33:13

    Google Scholar 

  • Rick CM, De Verna JW, Chetelat RT, Stevens MA (1987) Potential contributions of wide crosses to improvement of processing tomatoes. Acta Hortic 200:45–55

    Google Scholar 

  • Rick CM, De Verna JW, Chetelat RT (1990a) Experimental introgression to the cultivated tomato from related wild nightshades. Hortic Biotechnol 11:19–30

    Google Scholar 

  • Rick CM, Laterrot H, Philouze J (1990b) A revised key for theLycopersicon species. Tomato Genet Coop Rep 40:31

    Google Scholar 

  • Sakata Y, Nishio T, Narikawa T, Monna S (1991) Cold and disease resistance of somatic hybrids between tomato (Lycopersicon esculentum) andL. peruvianum. J. Jpn Soc Hortic Sci 60:329–335

    Google Scholar 

  • Sakomoto K, Taguchi T (1991) Regeneration of intergeneric somatic hybrid plants betweenLycopersicon esculentum andSolanum muricatum. Theor Appl Genet 81:509–513

    Google Scholar 

  • Schiller B, Herrmann RG, Melchers G (1982) Restriction endonuclease analysis of plastid DNA from tomato, potato and some of their somatic hybrids. Mol Gen Genet 186:453–459

    Google Scholar 

  • Schoenmakers HCH, Koornneef M, Alefs SJHM, Gerrits WFM, der Kop DV, Chérel I, Caboche M (1991) Isolation and characterization of nitrate-deficient mutants in tomato (Lycopersicon esculentum). Mol Gen Genet 227:485–464

    Google Scholar 

  • Schötz F (1968) Über Plastidenkonkurrenz beiOenothera. II. Biol Zentralbl 87:33–61

    Google Scholar 

  • Schweizer G, Ganal M, Ninnemann H, Hemleben V (1988) Species-specific DNA sequence for identification of somatic hybrids betweenLycopersicon esculentum andSolanum acaule. Theor Appl Genet 75:679–684

    Google Scholar 

  • Shahin EA (1985) Totipotency of tomato protoplasts. Theor Appl Genet 69:235–241

    Google Scholar 

  • Shepard JF, Bidney D, Barsby T, Kemble R (1983) Genetic transfer in plants through interspecific protoplast fusion. Science 219:683–688

    Google Scholar 

  • Smith PG (1944) Embryo culture of tomato species hybrid. Proc Am Soc Hortic Sci 44:413–416

    Google Scholar 

  • Smith SE (1989) Biparental inheritance of organelles and its implications in crop improvement. Plant Breed Rev 6:361–393

    Google Scholar 

  • Sugiura M (1992) The chloroplast genome. Plant Mol Biol 19:149–168

    Google Scholar 

  • Steele-Scott N, Tymms MJ, Possingham JV (1984) Plastid-DNA levels in the different tissues of potato. Planta 161:12–19

    Google Scholar 

  • Stevens A (1986) Inheritance of tomato fruit quality components. Plant Breed Rev 6:273–311

    Google Scholar 

  • Sundberg E, Glimelius K (1991) Effects of parental ploidy level and genetic divergence on chromosome elimination and chloroplast segregation in somatic hybrids withinBrassicaceae. Theor Appl Genet 83:81–88

    Google Scholar 

  • Sundberg E, Landgren M, Glimelius K (1987) Fertility and chromosome stability inBrassica napus resynthesised by protoplast fusion. Theor Appl Genet 75:96–104

    Google Scholar 

  • Sundberg E, Lagercrantz U, Glimelius K (1991) Effects of cell type used for fusion on chromosome elimination and chloroplast segregation inBrassica oleracea (+)Brassica napus hybrids. Plant Sci 78:89–98

    Google Scholar 

  • Tabaeizadeh Z, Perennes C, Bergounioux C (1985) Increasing the variability ofLycopersicon peruvianum Mill, by protoplast fusion withPetunia hybrida L. Plant Cell Rep 4:7–11

    Google Scholar 

  • Tan MMC, Boerrigter HS, Kool AJ (1987 a) A rapid procedure for plant regeneration from protoplasts isolated from suspension cultures and leaf mesophyll cells of wildSolanum species andLycopersicon pennellii. Plant Sci 49:63–72

    Google Scholar 

  • Tan MMC, Colijn-Hooymans CM, Lindhout WH, Kool AJ (1987b) A comparison of shoot regeneration from protoplasts and leaf discs of different genotypes of the cultivated tomato. Theor Appl Genet 75:105–108

    Google Scholar 

  • Tanksley SD, Loaiza-Figeroa F (1985) Gametophytic self-incompatibility is controlled by a single major locus on chromosome 1 inLycopersicon peruvianum. Proc Natl Acad Sci USA 82:5093–5096.

    Google Scholar 

  • Tanksley SD, Mutschler MA (1990) Linkage map of the tomato (Lycopersicon esculentum) (2n = 24). In: O'Brien SJ (ed) Genetic maps — locus map of complex genomes. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp 6.3–6.15

    Google Scholar 

  • Thomas BR, Pratt D (1981) Efficient hybridization betweenLycopersicon esculentum andL. peruvianum via embryo callus. Theor Appl Genet 59:215–219

    Google Scholar 

  • Turpin C (1986) Attempt of male cytoplasmic sterility introduction by intergeneric fusion in cultivated tomato. Acta Hortic 191:377–379

    Google Scholar 

  • Uddin MR, Berry SZ, Bisges AD (1988) An improved shoot regeneration system for somaclone production in tomatoes. Hortic Sci 23:1062–1064

    Google Scholar 

  • Vedel F, Chetrit P, Mathieu C, Pelletier G, Primard C (1986) Several different mitochondrial DNA regions are involved in intergenomic recombination inBrassica napus cybrid plants. Curr Genet 11:17–24

    Google Scholar 

  • Wachocki SE, Bonnema AB, O'Connell MA (1991) Comparison of the organization of the mitochondrial genome in tomato somatic hybrids and cybrids. Theor Appl Genet 81:420–427

    Google Scholar 

  • Wann EV, Johnson KW (1963) Intergeneric hybridization involving species ofSolanum andLycopersicon. Bot Gaz 124:451–455

    Google Scholar 

  • Wijbrandi J (1989) Isolation and characterisation of somatic hybrids betweenLycopersicon esculentum andLycopersicon peruvianum. PhD thesis, Agricultural University Wageningen, The Netherlands

    Google Scholar 

  • Wijbrandi J, Vos JGM, Koornneef M (1988) Transfer of regeneration capacity fromLycopersicon peruvianum toL. esculentum by protoplast fusion. Plant Cell Organ 12:193–196

    Google Scholar 

  • Wijbrandi J, Posthuma A, Kok JM, Rijken R, Vos JGM, Koornneef M (1990a) Assymetric somatic hybrids betweenLycopersicon esculentum and irradiatedLycopersicon peruvianum. 1. Cytogenetics and morphology. Theor Appl Genet 80:305–312

    Google Scholar 

  • Wijbrandi J, Zabel P, Koornneef M (1990b) Restriction fragment length polymorphism analysis of somatic hybrids betweenLycopersicon esculentum and irradiatedL. peruvianum: evidence for limited donor genome elimination and extensive chromosome rearrangements. Mol Gen Genet 222:270–277

    Google Scholar 

  • Wolters AMA, Schoenmakers HCH, Meulen-Muisers JJM vd, Knapp E vd, Derks FHM, Koornneef M, Zelcer A (1991) Limited DNA elimination from the irradiated potato parent in fusion products of albinoLycopersicon esculentum andSolanum tuberosum. Theor Appl Genet 83:225–232

    Google Scholar 

  • Zelcer A, Soferman O, Izhar S (1984) An in vitro screening for tomato genotypes exhibiting efficient shoot regeneration. JPlant Physiol 115:211–215

    Google Scholar 

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  1. Laboratoire de Biologie Cellulaire, INRA, route de Saint-Cyr, F-78026, Versailles Cédex, France

    C. Lefrançois, Y. Chupeau & J. P. Bourgin

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  1. C. Lefrançois
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  2. Y. Chupeau
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  3. J. P. Bourgin
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Communicated by H. F. Linskens

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Lefrançois, C., Chupeau, Y. & Bourgin, J.P. Sexual and somatic hybridization in the genusLycopersicon . Theoret. Appl. Genetics 86, 533–546 (1993). https://doi.org/10.1007/BF00838706

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  • DOI: https://doi.org/10.1007/BF00838706

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