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Production of somatic hybrid tissues following chemical and electrical fusion of protoplasts from albino cell suspensions ofMedicago sativa andM. borealis

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Abstract

Protoplasts isolated from cell suspensions of albinoMedicago borealis andM. sativa were fused chemically, using two methods, and electrically. Although a small scale method of chemical fusion gave the highest fusion frequency, electrofusion was the superior technique on the basis of throughput of green somatic hybrid cell colonies. Chlorophyll-containing tissues were confirmed as being somatic hybrid by isoenzyme and cytological analyses. This is the first report of the application of albino complementation to produce somatic hybrid cells in forage legumes.

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Abbreviations

AC:

alternating current

DC:

direct current

2,4-D:

2,4-dichlorophenoxyacetic acid

f.wt.:

fresh weight

PEG:

polyethylene glycol

K8P and K8:

Kao (1977) protoplast and cell culture media

MS:

Murashige and Skoog (1962) medium

UM:

Uchimiya and Murashige (1974) medium

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Author notes

  1. D. M. Gilmour

    Present address: Calgene Pacific, 16 Gipps Street, 3066, Collingwood, Victoria, Australia

Authors and Affiliations

  1. Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, University Park, Nottingham, UK

    D. M. Gilmour, M. R. Davey & E. C. Cocking

Authors
  1. D. M. Gilmour
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  2. M. R. Davey
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  3. E. C. Cocking
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Communicated by I. Potrykus

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Gilmour, D.M., Davey, M.R. & Cocking, E.C. Production of somatic hybrid tissues following chemical and electrical fusion of protoplasts from albino cell suspensions ofMedicago sativa andM. borealis . Plant Cell Reports 8, 29–32 (1989). https://doi.org/10.1007/BF00735772

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  • DOI: https://doi.org/10.1007/BF00735772

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