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Interspecific somatic hybridization betweenSolanum tuberosum L. andS. bulbocastanum dun. as a means of transferring nematode resistance

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Abstract

Interspecific somatic hybrids were produced between tetraploidSolanum tuberosum and a nematode-resistant accession of the diploid speciesSolanum bulbocastanum by protoplast fusion. Hybrid cells were selected using dual fluorescent labeling of protoplast preparations prior to fusion. Hybridity of regenerated plants was confirmed with a combination of morphological assessment, chromosome counting and isozyme analysis. Somatic hybrids had the same level of resistance to infection by race 1 of the nematodeMeloidogyne chitwoodi as theS. bulbocastanum parent used in the fusion. Some of the somatic hybrids were fertile as females when crossed with tetraploidS. tuberosum breeding lines. Thus, these hybrids can be used in a potato improvement program to incorporate a valuable pest resistance

Compendio

Híbridos somáticos interespecíficos fueron producidos, por fusión de protoplastos, entre el tetraploideSolanum tuberosum y una entrada de la especie diploideSolanum bulbocastanum resistente a los nematodos. Se seleccionaron células híbridas utilizando la marcación con sondas fluorescentes duales en las preparaciones de protoplastos antes de la fusión. Se confirmó la hibridación de las plantas regeneradas con una combinación de evaluación morfológica, contaje de cromosomas y análisis de isozimas.

Los híbridos somáticos tuvieron el mismo nivel de resistencia a la infectión por la raza 1 del nematodoMeloidogyne chitwoodi como el de los padresS. bulbocastanum utilizados en la fusión. Algunos de los híbridos somáticos fueron fértiles cuando se les usó como madres en cruzamientos con líneas de mejoramiento del tetraploideS. tuberosum. Por lo tanto, estos híbridos pueden ser usados en un programa relacionado al mejoramiento de la papa, para incorporar resistencia valiosa a la plaga.

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Authors and Affiliations

  1. UW Biotechnology Center, 1710 University Ave, 53705, Madison, WI

    S. Austin & J. D. Pohlman

  2. USDA, ARS, PWR, USA

    C. R. Brown

  3. Washington State University-IAREC, 99350, Prosser, WA

    H. Mojtahedi & G. S. Santo

  4. Department of Crop and Soil Sciences, MSU, 48824, East Lansing, MI

    D. S. Douches

  5. Department of Plant Pathology, USDA, ARS, 53706, Madison, WI

    J. P. Helgeson

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  1. S. Austin
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  2. J. D. Pohlman
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  3. C. R. Brown
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  4. H. Mojtahedi
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  5. G. S. Santo
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  6. D. S. Douches
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  7. J. P. Helgeson
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Austin, S., Pohlman, J.D., Brown, C.R. et al. Interspecific somatic hybridization betweenSolanum tuberosum L. andS. bulbocastanum dun. as a means of transferring nematode resistance. American Potato Journal 70, 485–495 (1993). https://doi.org/10.1007/BF02849067

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