Summary
Mesophyll protoplasts of tomato (Lycopersicon esculentum) and pepino (Solanum muricatum) were fused by using an electrofusion method and cultured in modified MS medium supplemented with naphthaleneacetic acid and kinetin, in which only pepino and somatic hybrid protoplasts could divide. Somatic hybrid plants showing intermediate characteristics in morphology were regenerated from the calli exhibiting vigorous growth in contrast with those of pepino. The hybrid nature of these plants was confirmed by cytological observation and biochemical analyses of phosphoglucomutase isozymes and the fraction-1-protein. The regenerated somatic hybrids grew to flowering stage and set fruits.
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Goldthwaite JJ, Bogorad L (1971) A one-step method for the isolation and determination of leaf riblose-1,5-diphosphate carboxylase. Anal Biochemn 41:57–66
Guri A, Levi A, Sink KC (1988) Morphological and molecular characterization of somatic hybrid plants between Lycopersicon esculentum and Solanum nigrum. Mol Gen Genet 212:191–198
Handley LW, Nickels RL, Cameron MW, Moore PP, Sink KC (1986) Somatic hybrid plants between Lycopersicon esculentum and Solanum licopersicoides. Theor Appl Genet 71:691–697
Hirai A (1982) Isoelectrofocusing of noncarboxymethylated fraction-1-protein from green callus. Plant Sci Lett 25:37–41
Kinsara A, Patnaik SN, Cocking EC, Power JB (1986) Somatic hybrid plants of Lycopersicon esculentum Mill. and Lycopersicon peruvianum Mill. J Plant Physiol 125:225–234
Melchers G, Sacristan MD, Holder AA (1978) Somatic hybrid plants of potato and tomato regeneration from fused protoplasts. Carlsberg Res Commun 43:203–218
Murashige T, Skoog F (1962) Revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
O'Connell MA, Hanson MR (1985) Somatic hybridization between Lycopersicon esculentum and Lycopersicon pennelli. Theor Appl Genet 70:1–12
O'Connell MA, Hanson MR (1986) Regeneration of somatic hybrid plants between Lycopersicon esculentum and Solanum rikii. Theor Appl Genet 72: 59–65
Radola BJ (1980) Ultrathin-layer isoelectric focusing in 50–100 μm polyacrylamide gels on silanized grass plates or polyester films. Electrophoresis 1:43–56
Rick CM (1982) The potential of exotic germ plasm for tomato improvement. In: Vasil IK, Scowcroft WR, Frey KJ (eds) Plant improvement and somatic cell genetics. Academic Press, New York, pp 1–28
Schieder O (1978) Somatic hybrids ofDatura innoxia Mill. + Datura discolor Bernh. and of Datura innoxia Mill. + Datura stramonium L. var. tatura L. I. Selection and characterization. Mol Gen Genet 162:113–119
Schweizer G, Ganal M, Ninnemann H, Hemleben V (1988) Species-specific DNA sequences for identification of somatic hybrids between Lycopersicon esculentum and Solanum acaule. Theor Appl Genet 75:379–384
Taguchi T, Kameya T (1986) Production of somatic hybrid plants between cabbage and Chinese cabbage through protoplast fusion. Jpn J Breed 36:185–189
Tanksley SD (1979) Linkage, chromosomal association, and expression of Adh-1 and Pgm-2 in tomato. Biochem Genet 17:1159–1167
Toriyama K, Hinata K, Kameya T (1987) Production of somatic hybrid plants, ‘Brassicomoricandia’, through protoplast fusion between Moricandia arvensis and Brassica oleracea. Plant Sci 48:123–128
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Communicated by K. Tsunewaki
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Sakomoto, K., Taguchi, T. Regeneration of intergeneric somatic hybrid plants between Lycopersicon esculentum and Solanum muricatum . Theoret. Appl. Genetics 81, 509–513 (1991). https://doi.org/10.1007/BF00219441
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DOI: https://doi.org/10.1007/BF00219441