Summary
Many somatic fusion hybrids have been produced between a dihaploid potato Solanum tuberosum and the sexually-incompatible wild species S. brevidens using both chemical and electrical fusion techniques. S. brevidens was resistant to both potato leaf roll virus (PLRV) and potato virus Y (PVY), the viruses being either at low (PLRV) or undetectable (PVY) concentrations as determined by enzyme-linked immunosorbent assay (ELISA). The S. tuberosum parent was susceptible to both viruses. A wide range of resistance, expressed as a decrease in virus concentration to both viruses was found amongst fusion hybrids, four of which were especially resistant. The practicality of introducing virus resistance from S. brevidens into cultivated potatoes by somatic hybridisation is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Austin S, Baer MA, Helgeson JP (1985) Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion. Plant Sci 39:75–82
Austin S, Ehlenfeldt MK, Baer MA, Helgeson JP (1986) Somatic hybrids produced by protoplast fusion between Solanum tuberosum and Solanum brevidens: Phenotypic variation under field conditions. Theor Appl Genet 71:682–690
Barker H, Harrison BD (1984) Expression of genes for resistance to potato virus Y in potato plants and protoplasts. Ann Appl Biol 105:539–545
Barker H, Harrison BD (1986) Restricted distribution of potato leaf roll virus antigen in resistant potato genotypes and its effect on transmission of the virus by aphids. Ann Appl Biol 109:595–604
Barsby TL, Shepard JF, Kemble RJ, Wong R (1984) Somatic hybridisation in the genus Solanum: S. tuberosum and S. brevidens. Plant Cell Rep 3:165–167
Brown PTH, Lörz H (1986) Molecular changes and possible origins of somaclonal variation. In: Semal J (ed) Somaclonal variation and crop improvement. Nihjoff, Dordrecht, pp 148–159
Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34:475–483
DeMaine MJ (1982) An evaluation of the use of dihaploids and unreduced gametes in breeding for quantitative resistance to potato pathogenes. J Agric Sci 99:79–83
Fish N, Karp A, Jones MGK (1987) Improved isolation of dihaploid S. tuberosum protoplasts and the production of somatic hybrids between dihaploid S. tuberosum and S. brevidens. In Vitro (in press)
Fish N, Karp A, Jones MGK (1988) Production of somatic hybrids by electrofusion in Solanum Theor Appl Genet 76 (in press)
Ffrench-Constant RH, Devonshire AL (1986) The effect of aphid immigration of the rate of selection of insecticide resistance in Myzus persicae by different classes of insecticides. Aspects Appl Biol 13:115–125
Gibson RW, Rice AD, Sawicki RM (1982) Effects of the pyrethroid deltamethrin on the acquisition and transmission of virus by Myzus persicae. Ann Appl Biol 100:49–54
Govier DA (1985) Purification and partial characterisation of beet mild yellowing virus and its serological detection in plants and aphids. Ann Appl Biol 107:439–447
Helgeson JP, Haberlach GT, Austin S (1986) Somatic hybrids between Solanum brevidens and Solanum tuberosum: Expression of a late blight resistance gene and potato leaf roll virus resistance. Plant Cell Rep 3:212–214
Hermsen JGT, Taylor LM (1979) Successful hybridization of non-tuberous Solanum etuberosum Lind, and tuber bearing S. pinnatisectum Dun. Euphytica 28:1–7
Jones RAC (1979) Resistance to potato leaf roll virus in Solanum brevidens. Potato Res 22:149–152
Kemble RJ, Barsby TL, Wong RSC, Shepard JF (1986) Mitochondrial DNA rearrangements in somatic hybrids of Solanum tuberosum and Solanum brevidens. Theor Appl Genet 72:787–793
Kennedy JS, Day MF, Eastop VF (1962) A conspectus of aphids as vectors of plant viruses. Commonwealth Institute of Entomology, London, p 114
Marco S (1980) The use of insecticides to control potato leaf roll virus in seed-potato crops on the Golan heights. Phytoparasitica 8:61–71
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Ross H (1986) Potato breeding — problems and perspectives. Parey, Berlin Hamburg, p 132
Tempelaar MJ, Jones MGK (1985a) Fusion characteristics of plant protoplasts in electric fields. Planta 165:205–216
Tempellaar MJ, Jones MGK (1985b) Directed electrofusion between protoplasts with different responses in a mass fusion systems. Plant Cell Rep 4:92–95
Author information
Authors and Affiliations
Additional information
Communicated by Y. Y. Gleba
Rights and permissions
About this article
Cite this article
Gibson, R.W., Jones, M.G.K. & Fish, N. Resistance to potato leaf roll virus and potato virus Y in somatic hybrids between dihaploid Solanum tuberosum and S. brevidens . Theoret. Appl. Genetics 76, 113–117 (1988). https://doi.org/10.1007/BF00288840
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00288840