Summary
Terbutryn-resistant plastids of the Nicotiana plumbaginifolia TBR2 mutant were introduced into N. tabacum plants by protoplast fusion following X-irradiation of TBR2 protoplasts. The N. tabacum chloroplast recipient line, SR1-A15, carried mutant (albino) plastids. Following protoplast fusion, potential cybrid cell lines with an N. tabacum (SR1-A15) nucleus and N. plumbaginifolia (TBR2) chloroplasts were identified by their green color. The presence of TBR2 plastids in regenerated green N. tabacum plants was confirmed by hybridization with a chloroplast DNA probe and by the modified chloroplast fluorescence transients characteristic of the TBR2 mutant. Cybrid plants were resistant to high levels of atrazine (10 kg/ha). The protruding stigma and shorter than normal filaments of the cybrids resulted in male sterility. In the cybrids atrazine resistance was associated with reduced vigour, suggesting a causal relationship.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arntzen CJ, Duesing JH (1983) Chloroplast-encoded herbicide resistance. In: Downey K, Voellmy RW, Ahmad F, Schultz J (eds) Advances in gene technology: Molecular genetics of plants and animals. Academic Press, New York, pp 273–294
Arntzen CJ, Pfister K, Steinback KE (1982) The mechanism of chloroplast triazine resistance: alterations in the site of herbicide action. In: LeBaron HM, Gressel J (eds) Herbicide resistance in plants. Wiley, New York, pp 186–214
Bandeen JD, Stephenson GR, Cowett ER (1982) Discovery and distribution of herbicide-resistant weeds in North America. In: LeBaron HM, Gressel J (eds) Herbicide resistance in plants. Wiley, New York, pp 9–30
Bayliss MW (1980) Chromosomal variation in plant tissues in culture. Int Rev Cytol [Suppl] 11A:113–144
Belliard G, Pelletier G, Vedel F, Quetier F (1978) Morphological characteristics and chloroplast DNA distribution in different cytoplasmic parasexual hybrids of Nicotiana tabacum. Mol Gen Genet 165:231–237
Beversdorf WD, Weiss-Lerman J, Erickson LR, Souza Machado V (1980) Transfer of cytoplasmically inherited triazine resistance from bird's rape to cultivated oilseed rape (Brassica camperstris and Brassica napus). Can J Genet Cytol 22:167–172
Binding H, Jain SM, Finger J, Mordhorst G, Nehls R, Gressel J (1982) Somatic hybridization of an atrazine resistant biotype of Solanum nigrum with Solanum tuberosum. Theor Appl Genet 63:273–277
Chaplin JF (1964) Use of male-sterile tobaccos in the production of hybrid seed. Tobacco Sci 8:105–109
Cséplő A, Nagy F, Maliga P (1984) Interspecific protoplast fusion to rescue a cytoplasmic lincomycin resistance mutation into fertile N. plumbaginifolia plants. Mol Gen Genet 198:7–11
Cséplő A, Mdgyesy P, Hideg E, Demeter S, Márton L, Maliga P (1985) Triazine-resistant Nicotiana mutants from photomixotrophic cell cultures. Mol Gen Genet 200:508–510
Fluhr R, Aviv D, Galun E, Edelman M (1984) Generation of heteroplastidic Nicotiana cybrids by protoplast fusion: Analysis for plastid recombinant types. Theor Appl Genet 67:491–497
Galun E, Atzee-Gonen P, Fluhr R, Edelman M, Aviv D (1982) Cytoplasmic hybridization in Nicotiana: mitochondrial DNA analysis in progenies resulting from fusion between protoplasts having different organelle constitutions. Mol Gen Genet 186:50–56
Gressel J, Ben-Sinai G (1985) Low intraspecific competitive fitness in a triazine-resistant, nearly nuclear-isogenic line of Brassica napus. Plant Sci 38:29–32
Hildebrand M, Jurgenson JE, Ramage RT, Bourque DP (1985) Derivation of a physical map of chloroplast DNA from Nicotiana tabacum by two=dimensional gel and computer-aided restriction analysis. Plasmid 14:64–79
Maliga P (1984) Cell culture produres for mutant selection and characterization in Nicotiana plumbaginifolia. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants, vol 1. Academic Press, Orlando, pp 552–562
Maliga P, Lõrz H, Lázár G, Nagy F (1982) Cytoplast-protoplast fusion for interspecific chloroplast transfer in Nicotiana. Mol Gen Genet 185:211–215
Medgyesy P, Fejes E, Maliga P (1985) Interspecific chloroplast recombination in a Nicotiana somatic hybrid. Proc Natl Acad Sci USA 82:6960–6964
Menczel L, Nagy F, Kiss Zs, Maliga P (1981) Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum and Nicotiana knightiana: Correlation of resistance to N. tabacum plastids. Theor Appl Genet 59:191–195
Menczel L, Galiba G, Nagy F, Maliga P (1982) Effect of radiation dosage on efficiency of chloroplast transfer by protoplast fusion in Nicotiana. Genetics 100:487–495
Menczel L, Nagy F, Lázár G, Maliga P (1983) Transfer of cytoplasmic male sterility by selection for streptomycin resistance after protoplast fusion in Nicotiana. Mol Gen Genet 189:365–369
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497
Nagy F, Lázár G, Menczel L, Maliga P (1983) A heteroplasmic state induced by protoplast fusion is a necessary condition for detecting rearrangements in Nicotiana mitochondrial DNA. Theor Appl Genet 66:203–207
Paterson DR, Arntzen CJ (1982) Detection of altered inhibition of photosystem II reactions in herbicide resistant plants. In: Edelman M, Hallick RB, Chua NH (eds) Methods in chloroplast molecular biology. Elsevier, Amsterdam, pp 109–118
Pelletier G, Primard C, Vedel F, Chetrit P, Remy R, Rousselle P, Renard M (1983) Intergeneric cytoplasmic hybridization in Cruciferae by protoplast fusion. Mol Gen Genet 191:244–250
Radosevich SR, Holt JS (1982) Physiological responses and fitness of susceptible and resistant weed biotypes to triazine herbicides. In: LeBaron HM, Gressel J (eds) Herbicide resistance in plants. Wiley, New York, pp 163–184
Sváb Z, Maliga P (1986) Nicotiana tabacum mutants with chloroplast encoded streptomycin resistance and pigment deficiency. Theor Appl Genet, in press
Author information
Authors and Affiliations
Additional information
Communicated by H. Sadler
Rights and permissions
About this article
Cite this article
Menczel, L., Polsby, L.S., Steinback, K.E. et al. Fusion-mediated transfer of triazine-resistant chloroplasts: Characterization of Nicotiana tabacum cybrid plants. Molec. Gen. Genet. 205, 201–205 (1986). https://doi.org/10.1007/BF00430427
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00430427