Summary
We obtained cybrid plants by electrofusing γ-irradiated protoplasts of a cytoplasmic male-sterile line “A-58 CMS” (Oryza sativa L.) and iodoacetamide (IOA)-treated protoplasts of the fertile (normal) rice cultivar “Fujiminori”. The cybridity of the plants was confirmed by mitochondrial (mt) DNA restriction endonuclease, and plasmid-like DNA analyses, and by isozyme, cytological and morphological investigations. The chromosome number of the cybrid plants is 24.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abdullah R, Cocking EC, Thompson JA (1986) Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/Technology 4:1087–1090
Aviv D, Galun E (1980) Restoration of fertility in cytoplasmic male sterile (CMS) Nicotiana sylvestris by fusion with X-irradiated N. tabacum protoplasts. Theor Appl Genet 58:121–127
Coulibaly MY, Demarly Y (1986) Regeneration of plantlets from protoplasts of rice, Oryza sativa L. Z Pflanzenzücht 96:79–81
Endo T, Komiya T, Masumitsu Y, Morikawa H, Yamada Y (1987) An intergeneric hybrid cell line of Duboisia hopwoodii and Nicotiana tabacum by protoplast fusion. J Plant Physiol 129:453–459
Fujimura T, Sakurai M, Akagi H, Negishi T, Hirose A (1985) Regeneration of rice plants from protoplasts. Plant Tissue Cult Lett 2:74–75
Galun E, Arzee-Genen P, Fluhr R, Edelman M, Aviv D (1982) Cytoplasmic hybridization in Nicotiana: Mitochondrial DNA analysis in progenies resulting from fusion between protoplasts having different organelle constitutions. Mol Gen Genet 186:50–56
Heyser JW, Dykes TA, Demott KJ, Nabors MW (1983) High frequency, long term regeneration of rice from callus culture. Plant Sci Lett 29:175–182
Kothari SL, Monte DC, Widholm JM (1986) Selection of Daucus carota somatic hybrids using drug resistance markers and characterization of their mitochondrial genomes. Theor Appl Genet 72:494–502
Kurata N, Iwata N, Omura T (1981) Karyotype analysis in rice II. Identification of extra chromosomes in trisomic plants and banding structure on some chromosomes. Jpn J Genet 56:41–50
Kyozuka J, Hayashi Y, Shimamoto K (1987) High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Mol Gen Genet 206:408–413
Menczel L, Nagy F, Lazar G, Maliga P (1983) Transfer of cytoplasmic male sterility by selection for streptomycin resistance after protoplast fusion in Nicotiana. Mol Gen Genet 189:365–369
Menczel L, Morgan A, Brown S, Maliga P (1987) Fusion-mediated combination of Ogura-type cytoplasmic male sterility with Brassica napus plastid using X-irradiated CMS protoplasts. Plant Cell Rep 6:98–101
Ozias-Akins P, Pring DR, Vasil IK (1987) Rearrangements in the mitochondrial genome of somatic hybrid cell lines of Pennisetum americamum (L.) K. Schum. + Panicum maximum Jacq. Theor Appl Genet 74:15–20
Shikanai T, Yang ZQ, Yamada Y (1987) Properties of the circular plasmid-like DNA B1 from mitochondria of cytoplasmic male-sterile rice. Plant Cell Physiol 28:1243–1251
Sidorov VA, Menczel L, Nagy F, Maliga P (1981) Chloroplast transfer in Nicotiana based on metabolic complementation between irradiated and iodoacetate-treated protoplasts. Planta 152:341–345
Smith SM, Street HE (1974) The decline of embryogenic potential as callus and suspension cultures of carrot (Daucus carota L.) are serially subcultured. Ann Bot 38:223–241
Terada R, Kyozuka J, Nishibayashi S, Shimamoto K (1987) Plantlet regeneration from somatic hybrids of rice (Oryza sativa L.) and barnyard grass (Echinochloa oryzicola Vasing). Mol Gen Genet 210:39–43
Toriyama K, Hinata K, Sasaki T (1986) Haploid and diploid plant regeneration from protoplasts of anther callus in rice. Theor Appl Genet 73:16–19
Vries SE de, Jacobsen E, Jones MGK, Loonen AEHM, Tempelaar MJ, Wijbrandi J, Freenstra WJ (1987) Somatic hybridization of amino acid analogue-resistant cell lines of potato (Solanum tuberosum L.) by electrofusion. Theor Appl Genet 73:451–458
Yamada Y, Yang ZQ, Tang DT (1985) Regeneration of rice plants from protoplasts. Rice Genet Newslett 2:94–95
Yamada Y, Yang ZQ, Tang DT (1986) Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.). Plant Cell Rep 4:85–88
Yang ZQ, Shikanai T, Yamada Y (1988) Asymmetric hybridization between cytoplasmic male-sterile (CMS) and fertile rice (Oryza sativa L.) protoplasts. Theor Appl Genet 76:801–808
Zelcer A, Aviv D, Galun E (1978) Interspecific transfer of cytoplasmic male sterility by fusion between protoplasts of normal Nicotiana sylvestris and X-ray irradiated protoplasts of male-sterile N. tabacum. Z Pflanzenphysiol 90:397–407
Author information
Authors and Affiliations
Additional information
Communicated by Hu Han
Rights and permissions
About this article
Cite this article
Yang, Z.Q., Shikanai, T., Mori, K. et al. Plant regeneration from cytoplasmic hybrids of rice (Oryza sativa L.). Theoret. Appl. Genetics 77, 305–310 (1989). https://doi.org/10.1007/BF00305820
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00305820