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Measles and Related Morbilliviruses

Methods and Protocols

  • Book
  • Jun 2024

Overview

Editors:
  1. Dzwokai Z. Ma

    1. Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Christian K. Pfaller

    1. Department of Molecular Medicine, Mayo Clinic, Rochester, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2808)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. A Quantitative Fusion Assay to Study Measles Virus Entry

    • Chanakha K. Navaratnarajah
    Pages 1-7

  3. A Semiquantitative Protein-Fragment Complementation Assay to Study Protein-Protein Interactions of the Polymerase Complex in Cellula

    • Joanna Brunel, Érica Urzua, Denis Gerlier, Louis-Marie Bloyet
    Pages 9-17

  4. Design and Execution of In Vitro Polymerase Assays for Measles Virus and Related Mononegaviruses

    • Robert M. Cox, Richard K. Plemper
    Pages 19-33

  5. Optical Control of Mononegavirus Gene Expression and Replication

    • Maino Tahara, Takashi Okura, Moritoshi Sato, Makoto Takeda
    Pages 35-56

  6. Generation of Defective Interfering Particles of Morbilliviruses Using Reverse Genetics

    • Linda J. Rennick, W. Paul Duprex, Natasha L. Tilston-Lunel
    Pages 57-70

  7. Molecular Analysis of Copy-Back Defective Interfering RNAs of Morbilliviruses

    • Felix G. M. Andres, Christian K. Pfaller
    Pages 71-88

  8. Applying Reverse Genetics to Study Measles Virus Interactions with the Host

    • Heidy Vera-Peralta, Valerie Najburg, Chantal Combredet, Thibaut Douché, Quentin Giai Gianetto, Mariette Matondo et al.
    Pages 89-103

  9. Identification of Host Factors of Paramyxoviruses by siRNA Genome-Wide Screens

    • Biaoguo Yan, Kristmundur Sigmundsson, Yaw Shin Ooi, Danielle E. Anderson
    Pages 105-120

  10. Dual RNA-seq Analysis of Patients’ Cells and Viral Genome After Measles Infection

    • Vasiliki Pogka, Andreas Mentis, Timokratis Karamitros
    Pages 121-127

  11. Investigating Liquid-Liquid Phase Separation in Virus-Generated Inclusion Bodies Using Fluorescence Recovery After Photobleaching of Fluorescently Labeled Host Proteins

    • Matthew B. T. Asuelime-Smith, Dzwokai Z. Ma
    Pages 129-140

  12. Culturing Immortalized Human Airway Epithelial Cells at an Air-Liquid Interface for Measles Virus Infection

    • Justin W. Kaufman, Brajesh K. Singh, Ni Li, Patrick L. Sinn
    Pages 141-152

  13. In Vitro Modeling of Feline Morbillivirus Infections Using Primary Feline Kidney Cells

    • Michael Sieg, Johannes Busch, Denny Böttcher, Thomas W. Vahlenkamp
    Pages 153-165

  14. Setup of Aerosol Delivery System to Prevent Measles Virus Infection in Nonhuman Primate Model

    • Olivier Reynard, Jérôme Montharu, Mathieu Iampietro, Claudia Gonzalez, Sandrine Le Guellec, Branka Horvat et al.
    Pages 167-175

  15. Histopathological Analysis of Brains from Dogs Infected with Canine Distemper Virus

    • José Manuel Verdes, Camila Larrañaga, Belén Varela, Victoria Iribarnegaray, Victoria Yozzi, Gimena Feijóo et al.
    Pages 177-195

  16. Canine Distemper Virus Pathogenesis in the Ferret Model

    • Oliver Siering, Bevan Sawatsky, Christian K. Pfaller
    Pages 197-208

  17. Measles Foci Reduction Neutralization Test (FRNT)

    • Sabine Santibanez, Annette Mankertz
    Pages 209-224

  18. Multiplex Bead Assay for the Serological Surveillance of Measles and Rubella

    • Melissa M. Coughlin, Gaby Smits, Zachary Matson, Rob van Binnendijk, Bettina Bankamp
    Pages 225-246

  19. Measles IgG Avidity Assay

    • Sara Mercader, Stephen Crooke
    Pages 247-264

  20. Back Matter

    Pages 265-266
    Download chapter PDF
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Keywords

About this book

This volume covers the latest protocols used to study the molecular biology of morbilliviruses, including cell entry, replication, and virus-host interactions. The chapters in this book also cover methods for studying morbillivirus infection through the use of novel tissue culture and in vivo models; analyzing the biological implications of infection by using primary tissues isolated from infected animals; and exploring diagnostic tools to evaluate humoral immunity against human-pathogenic measles virus (MeV). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and comprehensive, Measles and Related Morbilliviruses: Methods and Protocols is a valuable resource for researchers studying MeV,and will be useful to researchers studying other members of Paramyxoviridae and Mononegavirales. 


Editors and Affiliations

  • Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, USA

    Dzwokai Z. Ma

  • Department of Molecular Medicine, Mayo Clinic, Rochester, USA

    Christian K. Pfaller

Bibliographic Information

  • Book Title: Measles and Related Morbilliviruses

  • Book Subtitle: Methods and Protocols

  • Editors: Dzwokai Z. Ma, Christian K. Pfaller

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3870-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3869-9Due: 21 June 2024

  • Softcover ISBN: 978-1-0716-3872-9Due: 21 June 2024

  • eBook ISBN: 978-1-0716-3870-5Due: 21 June 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 266

  • Number of Illustrations: 3 b/w illustrations

  • Topics: Virology, Immunology

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