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Identification of Host Factors of Paramyxoviruses by siRNA Genome-Wide Screens

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Measles and Related Morbilliviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2808))

Abstract

Measles is a highly infectious disease that continues to spread mainly in developing countries, often resulting in child mortality. Despite the existence of effective vaccines, no specific antivirals are available as targeted therapy to combat measles virus (MeV). The implementation of genome-wide siRNA screens can provide a powerful platform to discover host factors that mediate MeV infection and replication, which could be essential to develop novel therapeutic strategies against this disease. Here, we describe a human genome-wide siRNA screen for MeV.

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Authors and Affiliations

  1. Programme in Emerging Infectious Diseases, Duke-National University of Singapore Medical School, Singapore, Singapore

    Biaoguo Yan & Yaw Shin Ooi

  2. Programme in Cardiovascular & Metabolic Disorders, Duke-National University of Singapore Medical School, Singapore, Singapore

    Kristmundur Sigmundsson

  3. Victorian Infectious Diseases Reference Laboratory, The Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia

    Danielle E. Anderson

Authors
  1. Biaoguo Yan
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  2. Kristmundur Sigmundsson
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  3. Yaw Shin Ooi
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  4. Danielle E. Anderson
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Corresponding author

Correspondence to Danielle E. Anderson .

Editor information

Editors and Affiliations

  1. Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA, USA

    Dzwokai Z. Ma

  2. Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA

    Christian K. Pfaller

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Yan, B., Sigmundsson, K., Ooi, Y.S., Anderson, D.E. (2024). Identification of Host Factors of Paramyxoviruses by siRNA Genome-Wide Screens. In: Ma, D.Z., Pfaller, C.K. (eds) Measles and Related Morbilliviruses. Methods in Molecular Biology, vol 2808. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3870-5_8

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  • DOI: https://doi.org/10.1007/978-1-0716-3870-5_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3869-9

  • Online ISBN: 978-1-0716-3870-5

  • eBook Packages: Springer Protocols

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