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Culturing Immortalized Human Airway Epithelial Cells at an Air-Liquid Interface for Measles Virus Infection

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Measles and Related Morbilliviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2808))

Abstract

Measles virus (MeV) infection of airway surface epithelial cells provides a site for final amplification before being released back into the environment via coughing and sneezing. Multiple cell lines have served as models of polarized epithelia for MeV infection, such as Caco2 cells (intestinal derived human epithelia) or MDCK cells (kidney derived canine epithelia). In this chapter, we describe the materials and air-liquid interface (ALI) culture conditions for maintaining four different cell lines derived from human airway epithelial cells: 16HBE14o-, Calu-3, H358, and NuLi-1. We provide methods for confirming transepithelial electrical resistance (TER) and preparing samples for microscopy as well as expected results from apical or basolateral MeV delivery. Polarized human airway derived cells serve as tissue culture models for investigating targeted questions about how MeV exits a human host. In addition, these methods are generalizable to studies of other respiratory viruses or the biology of ALI airway epithelial cells.

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Acknowledgments

We thank Cami Hippee and Ashley Cooney for their editorial comments. This work was supported by the National Institutes of Health R01 AI132402 and R01 HL105821.

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Authors and Affiliations

  1. Stead Family Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, IA, USA

    Justin W. Kaufman, Brajesh K. Singh & Patrick L. Sinn

  2. Scripps Research Institute, University of Florida, Jupiter, FL, USA

    Ni Li

Authors
  1. Justin W. Kaufman
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  2. Brajesh K. Singh
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  3. Ni Li
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  4. Patrick L. Sinn
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Corresponding author

Correspondence to Patrick L. Sinn .

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Editors and Affiliations

  1. Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA, USA

    Dzwokai Z. Ma

  2. Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA

    Christian K. Pfaller

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Kaufman, J.W., Singh, B.K., Li, N., Sinn, P.L. (2024). Culturing Immortalized Human Airway Epithelial Cells at an Air-Liquid Interface for Measles Virus Infection. In: Ma, D.Z., Pfaller, C.K. (eds) Measles and Related Morbilliviruses. Methods in Molecular Biology, vol 2808. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3870-5_11

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  • DOI: https://doi.org/10.1007/978-1-0716-3870-5_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3869-9

  • Online ISBN: 978-1-0716-3870-5

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