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Multiplex Bead Assay for the Serological Surveillance of Measles and Rubella

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Measles and Related Morbilliviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2808))

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Abstract

There is increasing interest in evaluating antibody responses to multiple antigen targets in a single assay. Immunity to measles and rubella are often evaluated together because immunity is provided through combined vaccines and because routine immunization efforts and surveillance for measles and rubella pathogens are combined in many countries. The multiplex bead assay (MBA) also known as the multiplex immunoassay (MIA) described here combines the measurement of measles- and rubella-specific IgG antibodies in serum quantitatively according to international serum standards and has been successfully utilized in integrated serological surveillance.

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Acknowledgments

We thank Logan Melot for contributing to the images of measles and rubella viruses in Fig. 1 using BioRender. We would like to thank Dr. Diana Martin and Dr. Gretchen Cooley for their continued collaboration and expert advice on integrated serosurveillance by MBA and use of DBS.

Funding

The findings and conclusions in this report are those of the authors (Melissa M. Coughlin, Zachary Matson, and Bettina Bankamp) and do not necessarily represent the official position of the Centers for Disease Control and Prevention, US Department of Health and Human Services.

Author information

Authors and Affiliations

  1. Centers for Disease Control and Prevention, National Center for Respiratory Diseases, Division of Viral Diseases, Viral Vaccine Preventable Diseases Branch, Atlanta, GA, USA

    Melissa M. Coughlin, Zachary Matson & Bettina Bankamp

  2. Center for Immunology and Infectious Diseases and Vaccines, National Institute for Public Health and Environment, Bilthoven, The Netherlands

    Gaby Smits & Rob van Binnendijk

Authors
  1. Melissa M. Coughlin
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  2. Gaby Smits
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  3. Zachary Matson
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  4. Rob van Binnendijk
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  5. Bettina Bankamp
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Corresponding author

Correspondence to Melissa M. Coughlin .

Editor information

Editors and Affiliations

  1. Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA, USA

    Dzwokai Z. Ma

  2. Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA

    Christian K. Pfaller

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Coughlin, M.M., Smits, G., Matson, Z., van Binnendijk, R., Bankamp, B. (2024). Multiplex Bead Assay for the Serological Surveillance of Measles and Rubella. In: Ma, D.Z., Pfaller, C.K. (eds) Measles and Related Morbilliviruses. Methods in Molecular Biology, vol 2808. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3870-5_17

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  • DOI: https://doi.org/10.1007/978-1-0716-3870-5_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3869-9

  • Online ISBN: 978-1-0716-3870-5

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