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Generation of Defective Interfering Particles of Morbilliviruses Using Reverse Genetics

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Measles and Related Morbilliviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2808))

Abstract

RNA viruses generate defective genomes naturally during virus replication. Defective genomes that interfere with the infection dynamics either through resource competition or by interferon stimulation are known as defective interfering (DI) genomes. DI genomes can be successfully packaged into virus-like-particles referred to as defective interfering particles (DIPs). Such DIPs can sustainably coexist with the full-length virus particles and have been shown to negatively impact virus replication in vitro and in vivo. Here, we describe a method to generate a clonal DI genome population by reverse genetics. This method is applicable to other RNA viruses and will enable assessment of DIPs for their antiviral properties.

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Acknowledgments

This work was funded by the Defense Advanced Research Projects Agency (DARPA) INTERfering and Co-Evolving Prevention and Therapy (INTERCEPT) program (HR0011940493), Boston University and the University of Pittsburgh.

Author information

Authors and Affiliations

  1. Center for Vaccine Research, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA

    Linda J. Rennick & W. Paul Duprex

  2. Department of Microbiology and Molecular Genetics, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USA

    Linda J. Rennick & W. Paul Duprex

  3. Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, USA

    Natasha L. Tilston-Lunel

Authors
  1. Linda J. Rennick
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  2. W. Paul Duprex
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  3. Natasha L. Tilston-Lunel
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Corresponding author

Correspondence to W. Paul Duprex .

Editor information

Editors and Affiliations

  1. Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA, USA

    Dzwokai Z. Ma

  2. Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA

    Christian K. Pfaller

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Rennick, L.J., Duprex, W.P., Tilston-Lunel, N.L. (2024). Generation of Defective Interfering Particles of Morbilliviruses Using Reverse Genetics. In: Ma, D.Z., Pfaller, C.K. (eds) Measles and Related Morbilliviruses. Methods in Molecular Biology, vol 2808. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3870-5_5

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  • DOI: https://doi.org/10.1007/978-1-0716-3870-5_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3869-9

  • Online ISBN: 978-1-0716-3870-5

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