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In Vitro Modeling of Feline Morbillivirus Infections Using Primary Feline Kidney Cells

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Measles and Related Morbilliviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2808))

Abstract

Domestic cats are the natural host of feline morbilliviruses (FeMV). Although other species can also be infected (such as dogs and opossums), no laboratory animal infection model is established so far. In vitro models for studying the molecular pathogenesis are therefore needed. For this purpose, propagation and titration of FeMV are key techniques. Unlike other morbilliviruses, such as canine distemper virus (CDV) or measles virus (MV), FeMV is a slow growing virus in cell culture and is difficult to titrate using classical plaque techniques. Here we describe methods for the efficient isolation of FeMV from natural sources (e.g., urine), the propagation of viral stocks, and their titration. In addition, we establish the generation of a three-dimensional infection model mimicking the feline tubular epithelium.

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Authors and Affiliations

  1. Faculty of Veterinary Medicine, Institute of Virology, Leipzig University, Leipzig, Germany

    Michael Sieg, Johannes Busch & Thomas W. Vahlenkamp

  2. Faculty of Veterinary Medicine, Institute of Veterinary Pathology, Leipzig University, Leipzig, Germany

    Denny Böttcher

Authors
  1. Michael Sieg
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  2. Johannes Busch
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  3. Denny Böttcher
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  4. Thomas W. Vahlenkamp
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Corresponding author

Correspondence to Michael Sieg .

Editor information

Editors and Affiliations

  1. Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA, USA

    Dzwokai Z. Ma

  2. Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA

    Christian K. Pfaller

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Sieg, M., Busch, J., Böttcher, D., Vahlenkamp, T.W. (2024). In Vitro Modeling of Feline Morbillivirus Infections Using Primary Feline Kidney Cells. In: Ma, D.Z., Pfaller, C.K. (eds) Measles and Related Morbilliviruses. Methods in Molecular Biology, vol 2808. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3870-5_12

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  • DOI: https://doi.org/10.1007/978-1-0716-3870-5_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3869-9

  • Online ISBN: 978-1-0716-3870-5

  • eBook Packages: Springer Protocols

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