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Histopathological Analysis of Brains from Dogs Infected with Canine Distemper Virus

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Measles and Related Morbilliviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2808))

Abstract

We describe the use of conventional histology and immunohistochemistry against canine distemper virus (CDV) to examine the brains of domestic dogs with a confirmed diagnosis of CDV infection. Histologically, to identify the main typical lesions, we used conventional H&E stain; to evaluate the progressive demyelination, we used Luxol Fast Blue stain; and to identify the presence of viral particles in these affected regions, we used immunohistochemistry against CDV. We confirm that the histopathological analysis of brains of distemper-infected dogs is a powerful tool to evaluate the typical brain lesions and could be used as an interesting natural model to continue studying the pathogenesis of canine distemper in different species and/or other morbillivirus infections, like measles.

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Acknowledgments

Victoria Iribarnegaray is PhD fellowship recipient of CAP-Udelar (Uruguay) and Camila Larrañaga and Belén Varela are MSc fellowship recipients of ANII and CAP-Udelar (Uruguay), respectively. José Manuel Verdes is Research Career Member of the National Research System (SNI-ANII), Uruguay.

Funding

This work was supported by the National Agency of Research and Innovation (ANII Fondo María Viñas 2019, Grant FMV-1-2019-1-155934), Committee of Scientific Research of the University of the Republic (CSIC, Udelar), and Programme for the Development of Basic Sciences (PEDECIBA), Uruguay.

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Authors and Affiliations

  1. Department of Pathobiology, Universidad de la República, Montevideo, Uruguay

    José Manuel Verdes, Camila Larrañaga, Belén Varela, Victoria Iribarnegaray, Victoria Yozzi & Kanji Yamasaki

  2. Veterinary Clinics & Hospital, Faculty of Veterinary, Universidad de la República, Montevideo, Uruguay

    Gimena Feijóo

Authors
  1. José Manuel Verdes
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  2. Camila Larrañaga
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  3. Belén Varela
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  4. Victoria Iribarnegaray
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  5. Victoria Yozzi
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  6. Gimena Feijóo
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  7. Kanji Yamasaki
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Corresponding author

Correspondence to José Manuel Verdes .

Editor information

Editors and Affiliations

  1. Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA, USA

    Dzwokai Z. Ma

  2. Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA

    Christian K. Pfaller

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Verdes, J.M. et al. (2024). Histopathological Analysis of Brains from Dogs Infected with Canine Distemper Virus. In: Ma, D.Z., Pfaller, C.K. (eds) Measles and Related Morbilliviruses. Methods in Molecular Biology, vol 2808. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3870-5_14

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  • DOI: https://doi.org/10.1007/978-1-0716-3870-5_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3869-9

  • Online ISBN: 978-1-0716-3870-5

  • eBook Packages: Springer Protocols

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