Abstract
Measles virus is one of the most contagious airborne human viruses which keeps causing outbreaks in numerous countries over the world despite the existence of an efficient vaccine. Fusion inhibitory lipopeptides were shown to inhibit viral entry into target cells, and their adequate administration into the respiratory tract may provide a novel preventive approach against airborne infections. Aerosol delivery presents the best administration route to deliver such preventive compounds to the upper and lower respiratory tract. This approach offers a conceptually new strategy to protect the population at risk against infection by respiratory viruses, including measles. It is a noninvasive needle-free approach, which may be used when antiviral protection is required, without any medical assistance. In this chapter, we describe the nebulization approach of lipopeptide compounds in nonhuman primates and the subsequent measles virus challenge.
References
Anderson RM, May RM (1982) Directly transmitted infections diseases: control by vaccination. Science 215:1053–1060. https://doi.org/10.1126/science.7063839
Figueira TN, Palermo LM, Veiga AS, Huey D, Alabi CA, Santos NC, Welsch JC, Mathieu C, Horvat B, Niewiesk S, Moscona A, Castanho MARB, Porotto M (2017) In vivo efficacy of measles virus fusion protein-derived peptides is modulated by the properties of self-assembly and membrane residence. J Virol 91:e01554–e01516. https://doi.org/10.1128/JVI.01554-16
Harrison SC (2008) Viral membrane fusion. Nat Struct Mol Biol 15:690–698. https://doi.org/10.1038/nsmb.1456
Martin AR, Finlay WH (2015) Nebulizers for drug delivery to the lungs. Expert Opin Drug Deliv 12:889–900. https://doi.org/10.1517/17425247.2015.995087
Mathieu C, Huey D, Jurgens E, Welsch JC, DeVito I, Talekar A, Horvat B, Niewiesk S, Moscona A, Porotto M (2015) Prevention of measles virus infection by intranasal delivery of fusion inhibitor peptides. J Virol 89:1143–1155. https://doi.org/10.1128/JVI.02417-14
Mitchell JP, Nagel MW, Nichols S, Nerbrink O (2006) Laser diffractometry as a technique for the rapid assessment of aerosol particle size from inhalers. J Aerosol Med 19:409–433. https://doi.org/10.1089/jam.2006.19.409
Pritchard JN, Hatley RH, Denyer J, Hollen D von (2018) Mesh nebulizers have become the first choice for new nebulized pharmaceutical drug developments. Ther Deliv 9:121–136. doi: https://doi.org/10.4155/tde-2017-0102
Reynard O, Gonzalez C, Dumont C, Iampietro M, Ferren M, Le Guellec S, Laurie L, Mathieu C, Carpentier G, Roseau G, Bovier FT, Zhu Y, Le Pennec D, Montharu J, Addetia A, Greninger AL, Alabi CA, Brisebard E, Moscona A, Vecellio L, Porotto M, Horvat B (2022) Nebulized fusion inhibitory peptide protects cynomolgus macaques from measles virus infection. Nat Commun 13:6439. https://doi.org/10.1038/s41467-022-33832-6
Welsch JC, Talekar A, Mathieu C, Pessi A, Moscona A, Horvat B, Porotto M (2013) Fatal measles virus infection prevented by brain-penetrant fusion inhibitors. J Virol 87:13785–13794. https://doi.org/10.1128/JVI.02436-13
Acknowledgments
We thank the animal experimentation PST-A team of Tours University for the realization of the animal experiments. We acknowledge the Servier Medical Art (smart.servier.com) for providing the images used for the schemas presented in the article. The study was supported by Region AURA (Pack Ambition Recherche, project AerVirStop-BH).
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Reynard, O. et al. (2024). Setup of Aerosol Delivery System to Prevent Measles Virus Infection in Nonhuman Primate Model. In: Ma, D.Z., Pfaller, C.K. (eds) Measles and Related Morbilliviruses. Methods in Molecular Biology, vol 2808. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3870-5_13
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DOI: https://doi.org/10.1007/978-1-0716-3870-5_13
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