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Genome-wide association mapping for stripe rust (Puccinia striiformis F. sp. tritici) in US Pacific Northwest winter wheat (Triticum aestivum L.)

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Potential novel and known QTL for race-specific all-stage and adult plant resistance to stripe rust were identified by genome-wide association mapping in the US PNW winter wheat accessions.

Abstract

Stripe rust (Puccinia striiformis F. sp. tritici; also known as yellow rust) is a globally devastating disease of wheat (Triticum aestivum L.) and a major threat to wheat production in the US Pacific Northwest (PNW), therefore both adult plant and all-stage resistance have been introduced into the winter wheat breeding programs in the PNW. The goal of this study was to identify quantitative trait loci (QTL) and molecular markers for these resistances through genome-wide association (GWAS) mapping in winter wheat accessions adapted to the PNW. Stripe rust response for adult plants was evaluated in naturally occurring epidemics in a total of nine environments in Washington State, USA. Seedling response was evaluated with three races under artificial inoculation in the greenhouse. The panel was genotyped with the 9K Illumina Wheat single nucleotide polymorphism (SNP) array and additional markers linked to previously reported genes and QTL for stripe rust resistance. The population was grouped into three sub-populations. Markers linked to Yr17 and previously reported QTL for stripe rust resistance were identified on chromosomes 1B, 2A, and 2B. Potentially novel QTL associated with race-specific seedling response were identified on chromosomes 1B and 1D. Potentially novel QTL associated with adult plant response were located on chromosomes 2A, 2B, 3B, 4A, and 4B. Stripe rust was reduced when multiple alleles for resistance were present. The resistant allele frequencies were different among sub-populations in the panel. This information provides breeders with germplasm and closely linked markers for stripe rust resistance to facilitate the transfer of multiple loci for durable stripe rust resistance into wheat breeding lines and cultivars.

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Acknowledgments

This project was supported by Washington State University (Hatch project #0232), the Washington Grain Commission Project # 5238, and a National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture. We greatly appreciate Dr. Linda Klein for her helpful review of and edits to the manuscript. We thank Ryan Higginbotham, Gary Shelton, and Kent Evans for maintenance of the field screening locations, Kerry Balow and Anmin Wan for assistance in the greenhouse screens, and Drs. Robert E. Allan and Clarence J. Peterson for their battles with stripe rust that led to the accumulation of so many minor genes for resistance in the PNW winter wheat germplasm.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Crop and Soil Sciences, Washington State University, Pullman, WA, 99164-6420, USA

    Y. Naruoka & A. H. Carter

  2. Wheat Genetics, Quality, Physiology, and Disease Research Unit, Agricultural Research Service, United States Department of Agriculture, Pullman, WA, 99164-6420, USA

    K. A. Garland-Campbell

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  1. Y. Naruoka
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  2. K. A. Garland-Campbell
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  3. A. H. Carter
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Correspondence to Y. Naruoka.

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Communicated by B. Keller.

Electronic supplementary material

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Table S1 List of germplasm accession used in the PNW winter panel to evaluate stripe rust resistance (XLSX 20 kb)

122_2015_2492_MOESM2_ESM.docx

Table S2 Pstv races of stripe rust identified in Pullman, Central Ferry and Mount Vernon in 2012 and 2013 and their associated virulence formulas (DOCX 500 kb)

122_2015_2492_MOESM3_ESM.docx

Table S3 Virulence and avirulence formula of Pstv-11, Pstv-37 and Pstv-51 for Yr3a, Yr10, Yr15, Yr24 and Yr25 (DOCX 17 kb)

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Naruoka, Y., Garland-Campbell, K.A. & Carter, A.H. Genome-wide association mapping for stripe rust (Puccinia striiformis F. sp. tritici) in US Pacific Northwest winter wheat (Triticum aestivum L.). Theor Appl Genet 128, 1083–1101 (2015). https://doi.org/10.1007/s00122-015-2492-2

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