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A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags

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Abstract

Bread wheat (Triticum aestivum L.) is a hexaploid species with a large and complex genome. A reference genetic marker map, namely the International Triticeae Mapping Initiative (ITMI) map, has been constructed with the recombinant inbred line population derived from a cross involving a synthetic line. But it is not sufficient for a full understanding of the wheat genome under artificial selection without comparing it with intervarietal maps. Using an intervarietal mapping population derived by crossing Nanda2419 and Wangshuibai, we constructed a high-density genetic map of wheat. The total map length was 4,223.1 cM, comprising 887 loci, 345 of which were detected by markers derived from expressed sequence tags (ESTs). Two-thirds of the high marker density blocks were present in interstitial and telomeric regions. The map covered, mostly with the EST-derived markers, approximately 158 cM of telomeric regions absent in the ITMI map. The regions of low marker density were largely conserved among cultivars and between homoeologous subgenomes. The loci showing skewed segregation displayed a clustered distribution along chromosomes and some of the segregation distortion regions (SDR) are conserved in different mapping populations. This map enriched with EST-derived markers is important for structure and function analysis of wheat genome as well as in wheat gene mapping, cloning, and breeding programs.

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Acknowledgments

This study was partially supported by the ‘973’ program (2006CB101700), the NSFC program (30430440, 30025030), and the ‘863’ program (2003AA207100).

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Authors and Affiliations

  1. The Applied Plant Genomics Laboratory, Crop Genomics and Bioinformatics Centre, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China

    Shulin Xue, Zhengzhi Zhang, Feng Lin, Zhongxin Kong, Yong Cao, Chunjun Li, Hongying Yi, Mingfeng Mei, Huilan Zhu, Jizhong Wu, Haibin Xu, Dongmei Zhao, Dagang Tian, Caiqin Zhang & Zhengqiang Ma

  2. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China

    Shulin Xue, Zhengzhi Zhang, Feng Lin, Zhongxin Kong, Yong Cao, Chunjun Li, Hongying Yi, Mingfeng Mei, Huilan Zhu, Jizhong Wu, Haibin Xu, Dongmei Zhao, Dagang Tian, Caiqin Zhang & Zhengqiang Ma

  3. College of Agricultural Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China

    Zhengqiang Ma

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  1. Shulin Xue
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  2. Zhengzhi Zhang
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  15. Zhengqiang Ma
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Correspondence to Zhengqiang Ma.

Additional information

Communicated by F. Salamini.

S. L. Xue and Z. Z. Zhang equally contributed to this article.

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Xue, S., Zhang, Z., Lin, F. et al. A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags. Theor Appl Genet 117, 181–189 (2008). https://doi.org/10.1007/s00122-008-0764-9

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  • DOI: https://doi.org/10.1007/s00122-008-0764-9

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