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Identifying QTL for high-temperature adult-plant resistance to stripe rust (Puccinia striiformis f. sp. tritici) in the spring wheat (Triticum aestivum L.) cultivar ‘Louise’

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Abstract

Over time, many single, all-stage resistance genes to stripe rust (Puccinia striiformis f. sp. tritici) in wheat (Triticum aestivum L.) are circumvented by race changes in the pathogen. In contrast, high-temperature, adult-plant resistance (HTAP), which only is expressed during the adult-plant stage and when air temperatures are warm, provides durable protection against stripe rust. Our objective was to identify major quantitative trait loci (QTL) for HTAP resistance to stripe rust in the spring wheat cultivar ‘Louise’. The mapping population consisted of 188 recombinant inbred lines (RIL) from a Louise (resistant) by ‘Penawawa’ (susceptible) cross. F5:6 lines were evaluated for stripe rust reaction under natural infection in replicated field trials at five locations in the US Pacific Northwest in 2007 and 2008. Infection type (IT) and disease severity were recorded for each RIL 2–4 times per location. In all environments, Penawawa, the susceptible parent, was rated with an IT ranging from 6 to 8 at all growth stages evaluated. In contrast, Louise, the resistant parent, was rated with an IT of 2 or 3 across growth stages. Distribution of IT values was bimodal, indicating a single major gene was affecting the trait. The parents and RIL population were evaluated with 295 polymorphic simple sequence repeat and one single nucleotide polymorphism markers. One major QTL, designated QYrlo.wpg-2BS, associated with HTAP resistance in Louise, was detected on chromosome 2BS (LOD scores ranging from 5.5 to 62.3 across locations and years) within a 16.9 cM region flanked by Xwmc474 and Xgwm148. SSR markers associated with QYrlo.wpg-2BS are currently being used in marker-based forward breeding strategies to transfer the target region into adapted germplasm to improve the durability of resistance in resulting cultivars.

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Acknowledgments

This research was supported by the National Research Initiative of USDA’s Cooperative State Research, Education and Extension Service, CAP Grant No. 2006-55606-16629 and Washington State University. We are grateful to David Vogan and Adrienne Burke for their assistance in molecular marker screening. We appreciate the USDA-ARS Western Regional Genotyping center for use of their genotyping equipment, along with the University of Idaho for allowing us to phenotype the RIL population at the Palouse Research, Extension and Education Center. We would like to thank Victor DeMacon for assistance in development of the mapping population as well as Gary Shelton and Dave Wood for assistance with field screening trials.

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Authors and Affiliations

  1. Department of Crop and Soil Sciences, Washington State University, Pullman, WA, 99164-6420, USA

    Arron Hyrum Carter, K. Garland-Campbell & K. K. Kidwell

  2. US Department of Agriculture, Agricultural Research Service, Wheat Genetics, Quality, Physiology and Disease Research Unit, Pullman, WA, 99164-6420, USA

    X. M. Chen & K. Garland-Campbell

  3. Deparment of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA

    X. M. Chen

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  1. Arron Hyrum Carter
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Correspondence to Arron Hyrum Carter.

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Communicated by I. Romagosa.

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Carter, A.H., Chen, X.M., Garland-Campbell, K. et al. Identifying QTL for high-temperature adult-plant resistance to stripe rust (Puccinia striiformis f. sp. tritici) in the spring wheat (Triticum aestivum L.) cultivar ‘Louise’. Theor Appl Genet 119, 1119–1128 (2009). https://doi.org/10.1007/s00122-009-1114-2

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