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Identification and mapping QTL for high-temperature adult-plant resistance to stripe rust in winter wheat (Triticum aestivum L.) cultivar ‘Stephens’

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Abstract

High-temperature adult-plant (HTAP) resistance from the winter wheat (Triticum aestivum) cultivar ‘Stephens’ has protected wheat crops from stripe rust caused by Puccinia striiformis f. sp. tritici for 30 years. The objectives of this study were to identify quantitative trait loci (QTL) for HTAP resistance in Stephens through genetic linkage analysis and identify DNA markers linked to the QTL for use in marker-assisted breeding. Mapping populations consisted of 101 recombinant inbred lines (RILs) through single-seed descent from ‘Stephens’ (resistant) × ‘Michigan Amber’ (susceptible). F5, F6 and F7 RILs were evaluated for stripe rust resistance at Pullman, WA in 1996, 1997 and 1998, respectively, whereas F8 RILs were evaluated at Mt Vernon, WA, USA in 2005. The 101 F8 RILs were evaluated with 250 resistance gene analog polymorphism (RGAP), 245 simple sequence repeat (SSR) and 1 sequence tagged site (STS) markers for genetic linkage map construction. Two QTL, which explained 48–61% of the total phenotypic variation of the HTAP resistance in Stephens, were identified. QYrst.wgp-6BS.1 was within a 3.9-cM region flanked by Xbarc101 and Xbarc136. QYrst.wgp-6BS.2 was mapped in a 17.5-cM region flanked by Xgwm132 and Xgdm113. Both two QTL were physically mapped to the short arm of chromosome 6B, but in different bins. Validation and polymorphism tests of the flanking markers in 43 wheat genotypes indicated that the molecular markers associated with these QTL should be useful in marker-assisted breeding programs to efficiently incorporate HTAP resistance into new wheat cultivars.

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Acknowledgments

This project was supported by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, grant number 2005-35301-15906. We are grateful to Dr. Clarice Coyne and Dr. Kevin McPhee for their critical review of the manuscript.

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Authors and Affiliations

  1. Department of Crop and Soil Sciences, Washington State University, Pullman, WA, 99164-6420, USA

    D. K. Santra, M. Santra, K. G. Campbell & K. K. Kidwell

  2. US Department of Agriculture, Agricultural Research Service, Wheat Genetics, Quality, Physiology and Disease Research Unit, Pullman, WA, 99164-6420, USA

    X. M. Chen & K. G. Campbell

  3. Department of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA

    X. M. Chen

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  1. D. K. Santra
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  2. X. M. Chen
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  3. M. Santra
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  5. K. K. Kidwell
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Correspondence to X. M. Chen.

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Communicated by R. Waugh.

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122_2008_820_MOESM1_ESM.rtf

Supplement Table 1. Pedigree, growth type and presence and absence of molecular markers for HTAP resistance QTL of Stephens in various wheat genotypes (RTF 210 kb)

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Santra, D.K., Chen, X.M., Santra, M. et al. Identification and mapping QTL for high-temperature adult-plant resistance to stripe rust in winter wheat (Triticum aestivum L.) cultivar ‘Stephens’. Theor Appl Genet 117, 793–802 (2008). https://doi.org/10.1007/s00122-008-0820-5

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