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Characterization of a major QTL for adult plant resistance to stripe rust in US soft red winter wheat

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Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is an important disease of soft red winter wheat in the eastern region of the USA. Pioneer 26R61 has provided effective resistance to stripe rust for 10 years. To elucidate the genetic basis of the resistance, a mapping population of 178 recombinant inbred lines (RILs) was developed using single-seed descent from a cross between Pioneer 26R61 and the susceptible cultivar AGS 2000. A genetic map with 895 markers covering all 21 chromosomes was used for QTL analysis. One major QTL was detected, explaining up to 56.0% of the mean phenotypic variation, flanked by markers Xbarc124 and Xgwm359, and assigned to the distal 22% of the short arm of wheat chromosome 2A. Evidence showed that it was different from Yr17 derived from Ae. ventricosa, the only formally named Yr gene in 2AS, and the QTL was temporarily designated as YrR61. In addition, a minor QTL, QYr.uga-6AS, probably conditioned high-temperature adult plant resistance. The QTL explained 6–7% of the trait variation. Preliminary test of the flanking markers for YrR61, in two cultivars and two promising breeding lines with Pioneer 26R61 in their pedigree, indicated that YrR61 was present in these cultivars and lines, and these markers could therefore be used in marker-assisted selection.

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Acknowledgments

This research was supported by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, CAP Grant No. 2006-55606-16629. The authors thank Dr. Xianming Chen, Wheat Genetics, Quality Physiology and Disease Research Unit, USDA-ARS, Department of Plant Pathology, Washington State University, Pullman, WA, USA, for kindly providing NILs for Yr17, and also acknowledge the help of Dr. Shengchu Wang, Department of Statistics and Bioinformatics Research Center, North Carolina State University, Raleigh, NC, USA, for suggestive instructions in using Windows QTL Cartographer 2.5 software. The authors are also very grateful to Dr. R.A. McIntosh, Plant Breeding Institute, the University of Sydney, Australia, for critical reviews of the manuscript.

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  1. Department of Crop and Soil Sciences, University of Georgia, Griffin Campus, Griffin, GA, 30223, USA

    Yuanfeng Hao, Zhenbang Chen, Yingying Wang, Dan Bland & Jerry Johnson

  2. Department of Plant Pathology, University of Georgia, Griffin Campus, Griffin, GA, 30223, USA

    James Buck

  3. USDA-ARS Plant Science Research, Department of Crop Science, North Carolina State University, Raleigh, NC, 27695, USA

    Gina Brown-Guedira

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  1. Yuanfeng Hao
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Correspondence to Jerry Johnson.

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Communicated by X. Xia.

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Hao, Y., Chen, Z., Wang, Y. et al. Characterization of a major QTL for adult plant resistance to stripe rust in US soft red winter wheat. Theor Appl Genet 123, 1401–1411 (2011). https://doi.org/10.1007/s00122-011-1675-8

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