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Quantitative trait loci for non-race-specific, high-temperature adult-plant resistance to stripe rust in wheat cultivar Express

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Abstract

Wheat cultivar Express has durable, high-temperature adult-plant (HTAP) resistance to stripe rust (Puccinia striiformis f. sp. tritici). To elucidate the genetic basis of the resistance, Express was crossed with ‘Avocet Susceptible’ (AVS). A mapping population of 146 F5 recombinant inbred lines (RILs) was developed using single-seed descent. The RILs were evaluated at two sites near Pullman in eastern Washington and one site near Mount Vernon in western Washington in 2005, and were evaluated near Pullman in 2006 under natural stripe rust infection of predominant races virulent on seedlings of Express. Infection type (IT) and disease severity (DS) were recorded three times for each line during each growing season. The DS data were used to calculate relative area under the disease progress curve (rAUDPC) values. Both IT and rAUDPC data showed continuous distributions, indicating that the Express HTAP resistance was controlled by quantitative trait loci (QTL). Resistance gene analog polymorphism (RGAP) and simple sequence repeat (SSR) techniques were used to map the HTAP resistance QTL. Three QTL were detected with significant additive effects, explaining 49.5–69.6% of the phenotypic variation for rAUDPC. Two of the QTL explained 30.8–42.7% of the phenotypic variation for IT. The three QTL were mapped to wheat chromosomes 6AS, 3BL and 1BL, and were designated as QYrex.wgp-6AS, QYrex.wgp-3BL and QYrex.wgp-1BL, respectively. QYrex.wgp-6AS and QYrex.wgp-3BL, which had higher effects than QYrex.wgp-1BL, were different from previously reported QTL/genes for adult-plant resistance. Markers Xgwm334–Xwgp56 and Xgwm299–Xwgp66 flanking the two major QTL were highly polymorphic in various wheat genotypes, suggesting that these markers are useful in marker-assisted selection.

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References

  • Bariana HS, Hayden MJ, Ahmed NU, Bell JA, Sharp PJ, McIntosh RA (2001) Mapping of durable adult plant and seedling resistances to stripe rust and stem rust diseases in wheat. Aust J Agric Res 52:1247–1255

    Article  CAS  Google Scholar 

  • Bariana HS, Parry N, Barclay IR, Longhman R, McLean RJ, Shankar M, Wilson RE, Willey NJ, Francki M (2006) Identification and characterization of stripe rust resistance gene Yr34 in common wheat. Theor Appl Genet 112:1143–1148

    Article  PubMed  CAS  Google Scholar 

  • Börner A, Röder MS, Unger O, Meinel A (2000) The detection and molecular mapping of a major gene for non-specific adult-plant disease resistance against stripe rust (Puccinia striiformis) in wheat. Theor Appl Genet 100:1095–1099

    Article  Google Scholar 

  • Bossolini E, Krattinger SG, Keller B (2006) Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii. Theor Appl Genet 113:1049–1062

    Article  PubMed  CAS  Google Scholar 

  • Boukhatem N, Baret PV, Mingeot D, Jacquemin JM (2002) Quantitative trait loci for resistance against yellow rust in two wheat-derived recombinant inbred line populations. Theor Appl Genet 104:111–118

    Article  PubMed  CAS  Google Scholar 

  • Castro AJ, Capettini F, Corey AE, Fillichkina T, Hayes PM, Kleinhofs A, Kudrna D, Richardson K, Sandoval-Islas S, Rossi C, Vivar H (2003) Mapping and pyramiding of qualitative and quantitative resistance to stripe rust in barley. Theor Appl Genet 107:922–930

    Article  PubMed  CAS  Google Scholar 

  • Chen FQ, Prehn D, Hayes PM, Mulrooney D, Corey A, Vivar HE (1994) Mapping genes for resistance barley stripe rust (Puccinia striiformis f. sp. hordei). Theor Appl Genet 88:215–219

    CAS  Google Scholar 

  • Chen XM (2005) Epidemiology and control of stripe rust [Puccinia striiformis f. sp. tritici] on wheat. Can J Plant Pathol 27:314–337

    Google Scholar 

  • Chen XM (2007) Challenges and solutions for stripe rust control in the United States. Aust J of Agric Res 58:648–655

    Article  Google Scholar 

  • Chen XM, Line RF (1995a) Gene action in wheat cultivars for durable high-temperature adult-plant resistance and interactions with race-specific, seedling resistance to stripe rust caused by Puccinia striiformis. Phytopathology 85:567–572

    Article  Google Scholar 

  • Chen XM, Line RF (1995b) Gene number and heritability of wheat cultivars with durable, high-temperature, adult-plant resistance and race-specific resistance to Puccinia striiformis. Phytopathology 85:573–578

    Article  Google Scholar 

  • Chen XM, Penman L (2006) Stripe rust epidemic, races of Puccinia striiformis in the United States in 2005. Phytopathology 96:S23

    Google Scholar 

  • Chen XM, Line RF, Leung H (1998) Genome scanning for resistance-gene analogs in rice, barley, and wheat by high-resolution electrophoresis. Theor Appl Genet 97:345–355

    Article  CAS  Google Scholar 

  • Chen XM, Moore M, Milus EA, Long DL, Line RF, Marshall D, Jackson L (2002) Wheat stripe rust epidemics and races of Puccinia striiformis f. sp. tritici in the United States in 2000. Plant Dis 86:39–46

    Article  Google Scholar 

  • Chen XM, Ling P, Wood DA, Moore MK, Pahalawatta V (2003) Epidemiology and control of wheat stripe rust in the United States, 2003. Ann Wheat Newsl 50:274–276

    Google Scholar 

  • Feuillet C, Schachermayr G, Keller B (1997) Molecular cloning of a new receptor-like kinase gene encoded at Lr10 disease resistance locus of wheat. Plant J 11:45–52

    Article  PubMed  CAS  Google Scholar 

  • Lagudah ES, McFadden H, Singh RP, Huerta-Espino J, Bariana HS, Sielmeyer W (2006) Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor Appl Genet 114:21–30

    Article  PubMed  CAS  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg I (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Leister D, Ballvora A, Salamini F, Gebhardt C (1996) A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plant. Nature Genet 14:421–429

    Article  PubMed  CAS  Google Scholar 

  • Lin F, Chen XM (2007) Genetics and molecular mapping of genes for race-specific all-stage resistance and non-race specific high-temperature adult-plant resistance to stripe rust in spring wheat cultivar Alpowa. Theor Appl Genet 114:1277–1287

    Article  PubMed  CAS  Google Scholar 

  • Lin F, Chen XM (2008) Molecular mapping of genes for race-specific overall resistance to stripe rust in wheat cultivar Express. Theor Appl Genet 116:797–806

    Article  PubMed  CAS  Google Scholar 

  • Line RF (2002) Stripe rust of wheat and barley in North America: a retrospective historical review. Annu Rev Phytopathol 40:75–118

    Article  PubMed  CAS  Google Scholar 

  • Line RF, Qayoum A (1992) Virulence, aggressiveness, evolution, and distribution of races of Puccinia striiformis (the cause of stripe rust of wheat) in North America, 1968-87. USDA-ARS, Technical Bulletin No. 1788, 44 pp

  • McIntosh RA, Devos KM, Dubcovsky J, Rogers WJ (2001) Catalogue of gene symbols for wheat: 2001 supplement (online). Available from http://wheat.pw.usda.gov/ggpages/wgc/2001upd.html [accessed 20 February 2007]

  • McIntosh RA, Hart GE, Devos KM, Rogers WJ (1999) Catalogue of gene symbols for wheat—1999 supplement (online). Available from http://wheat.pw.usda.gov/ggpages/wgc/99Supplement/ [accessed 20 February 2007]

  • Milus EA, Line RF (1986a) Number of genes controlling high-temperature, adult-plant resistance to stripe rust in wheat. Phytopathology 76:93–96

    Article  Google Scholar 

  • Milus EA, Line RF (1986b) Gene action for inheritance of durable, high-temperature, adult-plant resistance to stripe rust in wheat. Phytopathology 76:435–441

    Article  Google Scholar 

  • Pahalawatta V, Chen XM (2005) Inheritance and molecular mapping of barley genes conferring resistance to wheat stripe rust. Phytopathology 95:884–889

    Article  PubMed  CAS  Google Scholar 

  • Qayoum A, Line RF (1985) High-temperature, adult-plant resistance to stripe rust of wheat. Phytopathology 75:1121–1125

    Article  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2333

    PubMed  Google Scholar 

  • Rosewarne GM, Singh RP, Huerta-Espino J, William HM, Bouchet S, Cloutier S, McFadden H, Lagudah ES (2006) Leaf tip necrosis, molecular markers and β1-proteasome subunits associated with the slow rusting resistance genes Lr46/Yr29. Theor Appl Genet 112:500–508

    Article  PubMed  CAS  Google Scholar 

  • Santra DK, Santra M, Uauy C, Campbell KG, Chen XM, Dubcovsky J, Kidwell KK (2006) Identifying QTL for high-temperature adult-plant resistance to stripe rust in wheat (Triticum aestivum L.). Abstracts of plant & animal Genome XIV, January 14–18, 2006, San Diego, CA, p 179

  • Shi ZX, Chen XM, Line RF, Leung H, Wellings CR (2001) Development of resistance gene analog polymorphism markers for the Yr9 gene resistance to wheat stripe rust. Genome 44:509–516

    Article  PubMed  CAS  Google Scholar 

  • Singh RP (1992) Genetic association of leaf rust resistance gene Lr34 with adult-plant resistance to stripe rust in bread wheat. Phytopathology 82:835–838

    Article  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  PubMed  CAS  Google Scholar 

  • Stubbs RW (1985) Stripe rust. In: Roelfs AP, Bushnell WR (eds) The cereal rusts, vol 2. Disease, distribution, epidemiology, and control. Academic Press. Orlando, pp 61–101

  • Suenaga K, Singh RP, Huerta-Espino J, William HM (2003) Microsatellite markers for genes Lr34/Yr18 and other quantitative trait loci for leaf rust and stripe rust resistance in bread wheat. Phytopathology 93:881–890

    Article  PubMed  CAS  Google Scholar 

  • Tanksley SD (1993) Mapping polygenes. Annu Rev Genet 27:205–233

    Article  PubMed  CAS  Google Scholar 

  • Toojinda T, Broers LH, Chen XM, Hayes PM, Kleinhofs A, Korte J, Kudrna D, Leung H, Line RF, Powell W, Ramsay L, Vivar HE, Waugh R (2000) Mapping quantitative and qualitative disease genes in a double haploid population of barley (Hordeum vulgare). Theor Appl Genet 101:580–589

    Article  CAS  Google Scholar 

  • Uauy C, Brevis JC, Chen XM, Khan IA, Jackson LF, Chicaiza O, Distelfeld A, Fahima T, Dubcovsky J (2005) High-temperature adult plant (HTAP) stripe rust resistance gene Yr36 from Triticum turgidum ssp. dicoccoides is closely linked to the grain protein content locus Gpc-B1. Theor Appl Genet 112:97–105

    Article  PubMed  CAS  Google Scholar 

  • Wang SC, Basten J, Zeng ZB (2006) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC. [online]. Available from http://statgen.ncsu.edu/qtlcart/WQTLCart.htm [accessed 20 February 2007]

  • Wellings CR, McIntosh RA (1990) Puccinia striiformis f. sp. tritici in Australasia: pathogenic changes during the first 10 years. Plant Pathol 39:316–325

    Article  Google Scholar 

  • Worland AJ, Law CN (1986) Genetic analysis of chromosome 2D of wheat. I. The location of genes affecting height, day-length insensitivity, hybrid dwarfism and yellow rust resistance. Z. Pflanzenzücht 96:331–345

    Google Scholar 

  • Yan GP, Chen XM (2008) Identification of a quantitative trait locus for high-temperature adult-plant resistance against Puccinia striiformis f. sp. hordei in ‘Bancroft’ barley. Phytopathology 98:120–127

    Article  PubMed  CAS  Google Scholar 

  • Yan GP, Chen XM, Line RF, Wellings CR (2003) Resistance gene analog polymorphism markers co-segregating with the Yr5 gene for resistance to wheat stripe rust. Theor Appl Genet 106:636–643

    PubMed  CAS  Google Scholar 

  • Yang J, Bai G, Shaner GE (2005) Novel quantitative trait loci (QTL) for Fusarium head blight resistance in wheat cultivar Chokwang. Theor Appl Genet 111:1571–1579

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This research was supported by the US Department of Agriculture, Agricultural Research Service, Vogel Foundation, and Washington Wheat Commission. PPNS No. 0489, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences Research Center, Projects No. 5348-22000-010-00D, 13Z-3061-3824, and 13C-30613923, Washington State University, Pullman, WA 99164-6430, USA. We are grateful to D. Wood, L. Penman, and Y. M. Liu for their help in both the field and the greenhouse tests. We acknowledge Drs. R. P. Singh, J. Y. Zhuang, S. C. Wang, and K. L. Richardson, for technical assistance. We thank Drs. R. A. McIntosh and J. Nirmala for critical reviews of the manuscript.

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  1. F. Lin

    Present address: Bioscience and Technology College, Shenyang Agricultural University, Liaoning, 110161, People’s Republic of China

Authors and Affiliations

  1. Department of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA

    F. Lin & X. M. Chen

  2. Agricultural Research Service, Wheat Genetics, Quality, Physiology, and Disease Research Unit, US Department of Agriculture, Pullman, WA, 99164-6430, USA

    X. M. Chen

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  1. F. Lin
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Correspondence to X. M. Chen.

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Communicated by B. Keller.

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Lin, F., Chen, X.M. Quantitative trait loci for non-race-specific, high-temperature adult-plant resistance to stripe rust in wheat cultivar Express. Theor Appl Genet 118, 631–642 (2009). https://doi.org/10.1007/s00122-008-0894-0

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  • DOI: https://doi.org/10.1007/s00122-008-0894-0

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