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Molecular mapping of a gene for stripe rust resistance in spring wheat cultivar IDO377s

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Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases of wheat worldwide. The best strategy to control stripe rust is to grow resistant cultivars. One such cultivar resistant to most races in North America is ‘IDO377s’. To study the genetics of its resistance this spring wheat cultivar was crossed with ‘Avocet Susceptible’ (AvS). Seedlings of the parents, F2 plants, and F3 lines were tested under controlled greenhouse conditions with races PST-43 and PST-45 of P. striiformis f. sp. tritici. IDO377s carries a single dominant gene for resistance. Resistance gene analog polymorphism (RGAP) and simple sequence repeat (SSR) techniques were used to identify molecular markers linked to the resistance gene. A total of ten markers were identified, two of which flanked the locus at 4.4 and 5.5 cM. These flanking RGAP markers were located on chromosome 2B with nulli-tetrasomic lines of ‘Chinese Spring’. Their presence in the ditelosomic 2BL line localized them to the long arm. The chromosomal location of the resistance gene was further confirmed with two 2BL-specific SSR markers and a sequence tagged site (STS) marker previously mapped to 2BL. Based on the chromosomal location, reactions to various races of the pathogen and tests of allelism, the IDO377s gene is different from all previously designated genes for stripe rust resistance, and is therefore designated Yr43. A total of 108 wheat breeding lines and cultivars with IDO377s or related cultivars in their parentage were assayed to assess the status of the closest flanking markers and to select lines carrying Yr43. The results showed that the flanking markers were reliable for assisting selection of breeding lines carrying the resistance gene. A linked stripe rust resistance gene, previously identified as YrZak, in cultivar Zak was designated Yr44.

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Acknowledgments

Seed of Avocet S was originally provided by Dr. C. Wellings, Plant Breeding Institute, University of Sydney, Australia, and seed of North American breeding lines were provided by Dr. K. Kidwell and G. Shelton, Department of Crop and Soil Sciences, Washington State University. This research was supported by the US Department of Agriculture, Agricultural Research Service (Project No. 5348-22000-014-00D), Washington Wheat Commission (Project No. 13C-3061-3923), and Vogel Foundation (Project No. 13Z-3061-3824). PPNS No. 0529, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center, Project Number WNP00823, Washington State University, Pullman, WA 99164-6430, USA. We are grateful to D. Wood, Y.M. Liu, Dr. A.M. Wan, Dr. M.N. Wang and Dr. F. Lin for their technical assistance. We also would like to thank Drs. S. Hulbert, T. Peever and R.A. McIntosh for their critical review of the manuscript.

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Authors and Affiliations

  1. Department of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA

    P. Cheng & X. M. Chen

  2. Wheat Genetics, Quality, Physiology and Disease Research Unit, US Department of Agriculture, Agricultural Research Service, Pullman, WA, 99164-6430, USA

    X. M. Chen

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  1. P. Cheng
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Correspondence to X. M. Chen.

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Communicated by B. Keller.

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Cheng, P., Chen, X.M. Molecular mapping of a gene for stripe rust resistance in spring wheat cultivar IDO377s. Theor Appl Genet 121, 195–204 (2010). https://doi.org/10.1007/s00122-010-1302-0

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  • DOI: https://doi.org/10.1007/s00122-010-1302-0

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