Overview
- Editors:
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Huw D. Jones
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Peter R. Shewry
- Comprehensive guide to producing and analysing transgenic wheat, barley and oats
- Provides broad introduction and summary covering historical developments and future prospects
- Includes detailed protocols with troubleshooting tips for biolistic and Agrobacterium transformation
- Contains chapters on selectable markers and reporter genes in transgenic cereals
- In-depth examination of substantial equivalence and GM risk assessment
- Covers methods for up- and down-regulating gene expression
- Includes methods for molecular characterisation and field trialling GM plants
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Table of contents (20 protocols)
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Introduction
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- Paul A. Lazzeri*, Huw D. Jones
Pages 3-20
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Transformation and Regeneration
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- Huw D. Jones*, Caroline A. Sparks
Pages 23-37
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- Caroline A. Sparks, Huw D. Jones*
Pages 71-92
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- Huixia Wu, Angela Doherty, Huw D. Jones
Pages 93-103
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- Sujata Agarwal, Star Loar, Camille Steber, Janice Zale*
Pages 105-113
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- Thierry Risacher, Melanie Craze, Sarah Bowden, Wyatt Paul*, Tina Barsby
Pages 115-124
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- Wendy A. Harwood*, Mark A. Smedley
Pages 125-136
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- Wendy A. Harwood*, Joanne G. Bartlett, Silvia C. Alves, Matthew Perry, Mark A. Smedley, Nicola Leyl et al.
Pages 137-147
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- Shahina B. Maqbool*, Heng Zhong, Hesham F. Oraby, Mariam B. Sticklen
Pages 149-168
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Gene and Protein Expression
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Front Matter
Pages 170-170
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- Huw D. Jones*, Caroline A. Sparks
Pages 171-184
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- Silvia Travella*, Beat Keller
Pages 185-199
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Characterisation of Transgenic Plants
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Front Matter
Pages 202-202
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- Philippe Vain, Vera Thole
Pages 203-226
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- MarÃa Marcela Baudo*, Stephen J. Powers, Rowan A. C. Mitchell, Peter R. Shewry
Pages 247-272
About this book
Understanding the physical and genetic structure of cereal genomes and how defined coding and non-coding regions interact with the environment to determine a phenotype are key to the future of plant breeding and agriculture. The production and characteri- tion of transgenic plants is a powerful reverse genetic strategy increasingly used in cereals research to ascribe function to defined DNA sequences. However, the techniques and resources required to conduct these investigations have, until recently, been difficult to achieve or totally lacking in wheat, barley and oat. This book brings together the l- est protocols for the transformation, regeneration and selection using both biolistic and Agrobacterium tumefaciens appropriate for these three species. It includes two chapters describing in vitro Agrobacterium co-cultivation, one leading to germ line transformation with no need for tissue culture-based regeneration. In addition, it has several chapters dedicated to the manipulation of gene expression and characterisation of the recombinant locus and transgenic plants. Finally, it tackles the issues of GM risk assessment, field trials and substantial equivalence in terms of transcriptomics, proteomics and metabolomics. Although this book is dedicated to the temperate small grain cereals wheat, barley and oats, many of the techniques described could be readily adapted for other cereals or plants generally. We thank all the contributing authors for their timely and informative chapters, the staff of Humana Press, especially John Walker for their guidance, and Helen Jenkins for her proof-reading, word processing and administrative support. v Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix PART I.