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Transgenic Wheat, Barley and Oats: Production and Characterization

  • Paul A. Lazzeri*
  • Huw D. Jones
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 478)

Abstract

Ever since the first developments in plant transformation technology using model plant species in the early 1980s, there has been a body of plant science research devoted to adapting these techniques to the transformation of crop plants. For some crop species progress was relatively rapid, but in other crop groups such as the small grain cereals, which were not readily amenable to culture in vitro and were not natural hosts to Agrobacterium, it has taken nearly two decades to develop reliable and robust transformation methods.

In the following chapters of this book, transformation procedures for small grain cereals are presented, together with methods for gene and protein expression and the characterization of transgenic plants. In this introductory chapter we try to put these later chapters into context, giving an overview of the development of transformation technology for small grain cereals, discussing some of the pros and cons of the techniques and what limitations still exist.

Keywords

Small grain cereals transformation biolistics Agrobacterium tissue culture regeneration selection promoters reporter genes 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Agrasys S.LSpain
  2. 2.Centre for Crop Genetic Improvement, Department of Plant SciencesRothamsted ResearchHertfordshireUK

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