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Barley Transformation Using Agrobacterium-Mediated Techniques

  • Wendy A. Harwood*
  • Joanne G. Bartlett
  • Silvia C. Alves
  • Matthew Perry
  • Mark A. Smedley
  • Nicola Leyl
  • John W. Snape
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 478)

Abstract

Methods for the transformation of barley using Agrobacterium-mediated techniques have been available for the past 10 years. Agrobacterium offers a number of advantages over biolistic-mediated techniques in terms of efficiency and the quality of the transformed plants produced. This chapter describes a simple system for the transformation of barley based on the infection of immature embryos with Agrobacterium tumefaciens followed by the selection of transgenic tissue on media containing the antibiotic hygromycin. The method can lead to the production of large numbers of fertile, independent transgenic lines. It is therefore ideal for studies of gene function in a cereal crop system.

Key Words:

Barley transformation Agrobacterium tumefaciens transgenic plants hygromycin immature embryo 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Wendy A. Harwood*
    • 1
  • Joanne G. Bartlett
    • 1
  • Silvia C. Alves
    • 1
  • Matthew Perry
    • 1
  • Mark A. Smedley
    • 1
  • Nicola Leyl
    • 1
  • John W. Snape
    • 1
  1. 1.Department of Crop GeneticsJohn Innes CentreNorwich Research ParkUK

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