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Highly Efficient Agrobacterium-Mediated Transformation of Wheat Via In Planta Inoculation

  • Thierry Risacher
  • Melanie Craze
  • Sarah Bowden
  • Wyatt Paul*
  • Tina Barsby
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 478)

Abstract

This chapter details a reproducible method for the transformation of spring wheat using Agrobacterium tumefaciens via the direct inoculation of bacteria into immature seeds in planta as described in patent WO 00/63398(1. Transformation efficiencies from 1 to 30% have been obtained and average efficiencies of at least 5% are routinely achieved. Regenerated plants are phenotypically normal with 30–50% of transformation events carrying introduced genes at single insertion sites, a higher rate than is typically reported for transgenic plants produced using biolistic transformation methods.

Keywords:

Triticum aestivum Agrobacterium tumefaciens transformation immature embryos in planta inoculation 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Thierry Risacher
    • 1
  • Melanie Craze
    • 2
  • Sarah Bowden
    • 2
  • Wyatt Paul*
    • 1
  • Tina Barsby
    • 2
  1. 1.BiogemmaClermont-Ferrand Cedex 2
  2. 2.NIABCambridgeUK

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