Agrobacterium-Mediated Transformation of Bread and Durum Wheat Using Freshly Isolated Immature Embryos

  • Huixia Wu
  • Angela Doherty
  • Huw D. Jones
Part of the Methods in Molecular Biology™ book series (MIMB, volume 478)


Agrobacterium-mediated transformation of wheat is becoming a viable alternative to the more established biolistic protocols. It offers advantages in terms of simple, low-copy-number integrations and can be applied with similar efficiencies to specific durum wheat and spring and winter bread wheat types varieties.


Agrobacterium tumefaciens bread wheat durum wheat AGL1 inoculation immature embryo 



Rothamsted Research receives grant-aided support from the Biotechnology and Biological Sciences Research Council (BBSRC) of the UK. CIMMYT receives grant-aided support for wheat genetic transformation from the European Commission, the Japanese Government, and the Molecular Plant Breeding Cooperative Research Center.


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Huixia Wu
    • 1
  • Angela Doherty
    • 2
  • Huw D. Jones
    • 2
  1. 1.Genetic Resources and Enhancement UnitCIMMYTMexico DFMexico
  2. 2.Centre for Crop Genetic ImprovementDepartment of Plant Sciences, Rothamsted ResearchHarpendenUK

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