Agrobacterium-Mediated Transformation of Bread and Durum Wheat Using Freshly Isolated Immature Embryos
Agrobacterium-mediated transformation of wheat is becoming a viable alternative to the more established biolistic protocols. It offers advantages in terms of simple, low-copy-number integrations and can be applied with similar efficiencies to specific durum wheat and spring and winter bread wheat types varieties.
Keywords:Agrobacterium tumefaciens bread wheat durum wheat AGL1 inoculation immature embryo
Rothamsted Research receives grant-aided support from the Biotechnology and Biological Sciences Research Council (BBSRC) of the UK. CIMMYT receives grant-aided support for wheat genetic transformation from the European Commission, the Japanese Government, and the Molecular Plant Breeding Cooperative Research Center.
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