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Neurobiology

Methods and Protocols

  • Book
  • © 2024

Overview

Editors:
  1. Sebastian Dworkin

    1. Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Bundoora, Australia

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2746)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Plate-Based Assays for the Characterization of Mitochondrial and Cellular Phenotypes

    • Benjamin Henry Arnold, Oana Sanislav, Paul Robert Fisher, Sarah Jane Annesley
    Pages 1-20

  3. Behavioral Tests for Associative Learning in Caenorhabditis elegans

    • Aelon Rahmani, Anna McMillen, Ericka Allen, Caitlin Minervini, Yee Lian Chew
    Pages 21-46

  4. Mechanical Ablation of Larval Zebrafish Spinal Cord

    • Samuel Henry Crossman, Mitra Amiri Khabooshan, Sebastian-Alexander Stamatis, Celia Vandestadt, Jan Kaslin
    Pages 47-56

  5. High-Throughput Multiplex Immunohistochemistry of Glioma Organoids

    • Marija Dinevska, Samuel S. Widodo, Theo Mantamadiotis
    Pages 57-65

  6. The Bilateral Carotid Artery Stenosis (BCAS) Model of Vascular Dementia

    • Quynh Nhu Dinh, Thiruma Arumugam
    Pages 67-72

  7. In Situ Hybridization to Characterize Neurulation and Midbrain–Hindbrain Boundary Formation in Zebrafish

    • Jarrad Fuller, Sebastian Dworkin
    Pages 73-85

  8. Administering a Behavioral Test Battery in Rodents

    • Emily J. Jaehne, Michelle Corrone, Maarten van den Buuse
    Pages 87-100

  9. A Simple Method for Quantifying Larval Locomotion in Drosophila melanogaster

    • Jiayi Lin, Sarah Mele, Matthew D. W. Piper, Travis K. Johnson
    Pages 101-108

  10. Neural Stem/Progenitor Cell (NSPC) Extraction and Culture

    • Jemma Gasperoni, Sebastian Dworkin
    Pages 109-120

  11. Testing Prepulse Inhibition of Acoustic Startle in Rodents

    • Maarten van den Buuse, Emily J. Jaehne
    Pages 121-133

  12. Syngeneic Mouse Model of Glioblastoma: Intracranial Implantation of GL261 Cells

    • Paulina Kaminska, Salwador Cyranowski, Paulina Pilanc, Anna R. Malik
    Pages 135-146

  13. Measurement of Murine Neuromuscular Function Using the In Situ Preparation

    • S. T. C. Kucewicz, M. Borkowski, J. E. Church, C. van der Poel
    Pages 147-154

  14. Cryosectioning and Immunohistochemistry Using Frozen Adult Murine Brain Neural Tissue

    • Jemma Gasperoni, Sebastian Dworkin
    Pages 155-163

  15. Phenotypic Analysis of Early Neurogenesis in a Mouse Chimeric Embryo and Stem Cell-Based Neuruloid Model

    • Riley McMahon, V. Pragathi Masamsetti, Patrick P. L. Tam
    Pages 165-177

  16. Active Induction of a Multiple Sclerosis-Like Disease in Common Laboratory Mouse Strains

    • Jacqueline M. Orian, Dain L. Maxwell, Vernise J. T. Lim
    Pages 179-200

  17. Immunohistochemical Analysis of the Drosophila Larval Neuromuscular Junction

    • Yichen Sun, Yu Zhao, Travis K. Johnson, Wei Xie
    Pages 201-211

  18. Neural Endophenotype Assessment in Zebrafish Larvae Using Optomotor and ZebraBox Locomotion Assessment

    • Jiaheng Xie, Patrick Goodbourn, Tamar Sztal, Patricia R. Jusuf
    Pages 213-224

  19. The Photothrombotic Model of Ischemic Stroke

    • David E. Wong Zhang, Shenpeng R. Zhang, Hyun Ah. Kim, Christopher G. Sobey, T. Michael De Silva
    Pages 225-235

  20. Corrections to: Neurobiology: Methods and Protocols

    • Sebastian Dworkin
    Pages C1-C1
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Keywords

About this book

This volume will serve as a guide for students in the field of neurobiology, and be a bridge between basic science researchers, doctors, and surgeons in clinical practice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Neurobiology: Methods and Protocols aims to ensure successful results in the further study of this vital field.



Editors and Affiliations

  • Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Bundoora, Australia

    Sebastian Dworkin

Bibliographic Information

  • Book Title: Neurobiology

  • Book Subtitle: Methods and Protocols

  • Editors: Sebastian Dworkin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3585-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3584-1Published: 10 December 2023

  • Softcover ISBN: 978-1-0716-3587-2Due: 12 February 2024

  • eBook ISBN: 978-1-0716-3585-8Published: 09 December 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 239

  • Number of Illustrations: 5 b/w illustrations, 54 illustrations in colour

  • Topics: Neurosciences

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