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Neural Stem/Progenitor Cell (NSPC) Extraction and Culture

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Neurobiology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2746))

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Abstract

Neural stem–progenitor cells (NSPCs) are multipotent, self-renewing cells that generate radial glial cells (RGC). RGCs then give rise to neurons and glia during neural development. Here, we describe the process of NSPC isolation and culturing to form clonal aggregates termed neurospheres. There are multiple assays outlined in this chapter that allow us to quantify differences in proliferation, self-renewal potential, and differentiation of these cells.

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Authors and Affiliations

  1. Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Melbourne, VIC, Australia

    Jemma Gasperoni & Sebastian Dworkin

Authors
  1. Jemma Gasperoni
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  2. Sebastian Dworkin
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Corresponding author

Correspondence to Sebastian Dworkin .

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Editors and Affiliations

  1. Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Bundoora, VIC, Australia

    Sebastian Dworkin

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Gasperoni, J., Dworkin, S. (2024). Neural Stem/Progenitor Cell (NSPC) Extraction and Culture. In: Dworkin, S. (eds) Neurobiology. Methods in Molecular Biology, vol 2746. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3585-8_9

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  • DOI: https://doi.org/10.1007/978-1-0716-3585-8_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3584-1

  • Online ISBN: 978-1-0716-3585-8

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