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Cryosectioning and Immunohistochemistry Using Frozen Adult Murine Brain Neural Tissue

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Neurobiology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2746))

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Abstract

Cryopreservation and immunohistochemistry offer a comprehensive, robust, and simple methodology to investigate neural patterning and cellular function. Rapid freezing of the whole brain allows excellent preservation of neural ultrastructure and tissue architecture without destroying sensitive protein epitopes that are often compromised following standard paraffin embedding histological techniques. Here, we present a rapid and simple protocol for employing cryosectioning and subsequent immunohistochemistry in the study of adult murine brain neural tissue.

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Authors and Affiliations

  1. Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Melbourne, VIC, Australia

    Jemma Gasperoni & Sebastian Dworkin

Authors
  1. Jemma Gasperoni
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  2. Sebastian Dworkin
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Corresponding author

Correspondence to Sebastian Dworkin .

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Editors and Affiliations

  1. Department of Microbiology, Anatomy, Physiology and Pharmacology, La Trobe University, Bundoora, VIC, Australia

    Sebastian Dworkin

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Gasperoni, J., Dworkin, S. (2024). Cryosectioning and Immunohistochemistry Using Frozen Adult Murine Brain Neural Tissue. In: Dworkin, S. (eds) Neurobiology. Methods in Molecular Biology, vol 2746. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3585-8_13

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  • DOI: https://doi.org/10.1007/978-1-0716-3585-8_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3584-1

  • Online ISBN: 978-1-0716-3585-8

  • eBook Packages: Springer Protocols

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