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Reactive Oxygen Species in Plants

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Amna Mhamdi

    1. VIB Center for Plant Systems Biology, Ghent University, Zwijnaarde, Belgium

    View editor publications

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2526)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Strategies to Induce ROS Production

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Modification of Chloroplast Antioxidant Capacity by Plastid Transformation

      • Shengchun Li, Pan Shen, Bipeng Wang, Xiujie Mu, Mimi Tian, Tao Chen et al.
      Pages 3-13

    3. Analysis of Ascorbate Metabolism in Arabidopsis Under High-Light Stress

      • Takanori Maruta, Takahiro Ishikawa
      Pages 15-24

    4. Genetic Manipulation of Reactive Oxygen Species (ROS) Homeostasis Utilizing CRISPR/Cas9-Based Gene Editing in Rice

      • Sheng Xu, Tao Chen, Mimi Tian, Marie-Sylviane Rahantaniaina, Linlin Zhang, Ren Wang et al.
      Pages 25-41

    5. Studying Plant Stress Reactions In Vivo by PAM Chlorophyll Fluorescence Imaging

      • Alexey Shapiguzov, Jaakko Kangasjärvi
      Pages 43-61

  3. Methods to Visualize ROS and to Detect Changes in Redox Homeostasis

    1. Front Matter

      Pages 63-63
      Download chapter PDF

    2. Live Monitoring of ROS-Induced Cytosolic Redox Changes with roGFP2-Based Sensors in Plants

      • José Manuel Ugalde, Lara Fecker, Markus Schwarzländer, Stefanie J. Müller-Schüssele, Andreas J. Meyer
      Pages 65-85

    3. Quantitative Measurement of Ascorbate and Glutathione by Spectrophotometry

      • Graham Noctor, Amna Mhamdi
      Pages 87-96

    4. Measurement of NAD(P)H and NADPH-Generating Enzymes

      • Amna Mhamdi, Frank Van Breusegem, Graham Noctor
      Pages 97-106

    5. Quantitative Analysis for ROS-Producing Activity and Regulation of Plant NADPH Oxidases in HEK293T Cells

      • Sachie Kimura, Hidetaka Kaya, Kenji Hashimoto, Michael Wrzaczek, Kazuyuki Kuchitsu
      Pages 107-122

  4. Small-Scale Targeted Analysis of ROS Accumulation During Stress and Effects on Plant Physiology

    1. Front Matter

      Pages 123-123
      Download chapter PDF

    2. Estimation of the Level of Abasic Sites in Plant mRNA Using Aldehyde Reactive Probe

      • Jagna Chmielowska-Bąk, Karolina Izbiańska-Jankowska, Joanna Deckert
      Pages 125-134

    3. A Simplified Method to Assay Protein Carbonylation by Spectrophotometry

      • Corentin Moreau, Emmanuelle Issakidis-Bourguet
      Pages 135-141

    4. In Vitro Biochemical Analysis of Recombinant Plant Proteins Under Oxidation

      • Zeya Chen, Jingjing Huang
      Pages 143-160

    5. Methods to Analyze the Redox Reactivity of Plant Proteins

      • Thualfeqar Al-Mohanna, George V. Popescu, Sorina C. Popescu
      Pages 161-179

    6. Determination of ROS-Induced Lipid Peroxidation by HPLC-Based Quantification of Hydroxy Polyunsaturated Fatty Acids

      • Brigitte Ksas, Michel Havaux
      Pages 181-189

    7. Detection of Lipid Peroxidation-Derived Free Azelaic Acid, a Biotic Stress Marker and Other Dicarboxylic Acids in Tobacco by Reversed-Phase HPLC-MS Under Non-derivatized Conditions

      • Attila L. Ádám, György Kátay, András Künstler, Lóránt Király
      Pages 191-200

    8. Determination of Reactive Carbonyl Species, Which Mediate Reactive Oxygen Species Signals in Plant Cells

      • Jun’ichi Mano, Md. Sanaullah Biswas, Koichi Sugimoto, Yoshiyuki Murata
      Pages 201-213

    9. Measuring Stress-Induced Changes in Defense Phytohormones and Related Compounds

      • Caroline Lelarge-Trouverie, Amna Mhamdi, Florence Guérard, Graham Noctor
      Pages 215-223
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Keywords

About this book

This detailed volume explores techniques to study reactive oxygen species (ROS) in plants and to characterize their roles in development and stress responses. Beginning with a section on strategies to induce ROS production, the book continues with methods to visualize ROS and detect changes in redox homeostasis, small-scale and targeted analyses for investigating the effects of ROS accumulation during stress on plant physiology and metabolism, as well as systems biology approaches to understand ROS functions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Reactive Oxygen Species in Plants: Methods and Protocols serves as a vital resource that any researcher, and in particular young researchers, can use and adapt to further our knowledge of this dynamic area of plant science.

Editors and Affiliations

  • VIB Center for Plant Systems Biology, Ghent University, Zwijnaarde, Belgium

    Amna Mhamdi

Bibliographic Information

  • Book Title: Reactive Oxygen Species in Plants

  • Book Subtitle: Methods and Protocols

  • Editors: Amna Mhamdi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2469-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2468-5Published: 04 June 2022

  • Softcover ISBN: 978-1-0716-2471-5Published: 05 June 2023

  • eBook ISBN: 978-1-0716-2469-2Published: 03 June 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 298

  • Number of Illustrations: 17 b/w illustrations, 36 illustrations in colour

  • Topics: Plant Biochemistry, Plant Physiology, Plant Sciences

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