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Studying Plant Stress Reactions In Vivo by PAM Chlorophyll Fluorescence Imaging

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Reactive Oxygen Species in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2526))

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Abstract

Plant photosynthetic and mitochondrial electron transfer chains (ETCs) are delicate environmental sensors and active players in stress acclimation. The performance of photosynthetic ETC can be deduced from chlorophyll a fluorescence. This makes chlorophyll fluorescence imaging a powerful tool to study plant stress in vivo. Many stress treatments enhance production of reactive oxygen species (ROS) by photosynthetic or mitochondrial ETCs. These ROS affect cellular metabolism and signalling. Generation of ROS can be manipulated in planta by specific pharmacological treatments with methyl viologen (MV), antimycin A (AA), myxothiazol (myx), and salicylhydroxamic acid (SHAM). This chapter describes how chlorophyll fluorescence imaging together with pharmacological treatments can be employed to probe ROS-dependent plant stress reactions in vivo.

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Acknowledgments

We thank Dr. Fuqiang Cui for his assistance in developing the leaf disc assays. We are grateful to Dr. Zuzana Benedikty for optimizing the FluorCam protocol and for revising the manuscript and to Dr. Erhard Pfündel, Dr. Mikael Brosché, and Tuomas Puukko for their helpful comments on the manuscript.

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Authors and Affiliations

  1. Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, and Viikki Plant Science Center, University of Helsinki, Helsinki, Finland

    Alexey Shapiguzov & Jaakko Kangasjärvi

  2. Natural Resources Institute Finland (Luke), Piikkiö, Finland

    Alexey Shapiguzov

Authors
  1. Alexey Shapiguzov
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  2. Jaakko Kangasjärvi
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Corresponding author

Correspondence to Alexey Shapiguzov .

Editor information

Editors and Affiliations

  1. VIB Center for Plant Systems Biology, Ghent University, Zwijnaarde, Belgium

    Amna Mhamdi

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Shapiguzov, A., Kangasjärvi, J. (2022). Studying Plant Stress Reactions In Vivo by PAM Chlorophyll Fluorescence Imaging. In: Mhamdi, A. (eds) Reactive Oxygen Species in Plants. Methods in Molecular Biology, vol 2526. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2469-2_4

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  • DOI: https://doi.org/10.1007/978-1-0716-2469-2_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2468-5

  • Online ISBN: 978-1-0716-2469-2

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