Abstract
Pyridine nucleotides (NAD(H) and NADP(H)) are key redox carriers in cells and may also have other functions related to stress. These two molecules are crucial in linking metabolism to electron transport chains in photosynthesis and respiration, but they are also critical for ensuring redox signaling and homeostasis during episodes of stress. This is especially the case for NADPH, which must be generated from its oxidized form, NADP+, by key dehydrogenases. Here, we describe methods that can be used to assay contents and redox states of NAD(H) and NADP(H), as well as simple assays to measure the capacity of two key NADPH-generating enzymes.
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Acknowledgments
Work in the GN laboratory is supported by the French Agence Nationale de la Recherche HIPATH project (ANR-17-CE20-0025) and by the Institut Universitaire de France (IUF).
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Mhamdi, A., Van Breusegem, F., Noctor, G. (2022). Measurement of NAD(P)H and NADPH-Generating Enzymes. In: Mhamdi, A. (eds) Reactive Oxygen Species in Plants. Methods in Molecular Biology, vol 2526. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2469-2_7
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DOI: https://doi.org/10.1007/978-1-0716-2469-2_7
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