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In Vitro Biochemical Analysis of Recombinant Plant Proteins Under Oxidation

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Reactive Oxygen Species in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2526))

Abstract

Biochemical analysis is crucial for determining protein functionality changes during various conditions, including oxidative stress conditions. In this chapter, after giving brief guidelines for experimental design, we provide step-by-step instructions to purify recombinant plant proteins from E. coli, to prepare reduced and oxidized proteins for activity assay, and to characterize the protein under reducing and oxidizing conditions, with a focus on thiol-based oxidative modifications, like S-sulfenylation and disulfide formations.

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Acknowledgments

This work was supported by the Research Foundation-Flanders (FWO) senior postdoctoral fellowship (no.1227020N to J.H.), the China Scholarship Council (CSC, no. 201906300078 to Z.C.).

Author information

Authors and Affiliations

  1. Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium

    Zeya Chen & Jingjing Huang

  2. Center for Plant Systems Biology, VIB, Ghent, Belgium

    Zeya Chen & Jingjing Huang

Authors
  1. Zeya Chen
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  2. Jingjing Huang
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Corresponding author

Correspondence to Jingjing Huang .

Editor information

Editors and Affiliations

  1. VIB Center for Plant Systems Biology, Ghent University, Zwijnaarde, Belgium

    Amna Mhamdi

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Chen, Z., Huang, J. (2022). In Vitro Biochemical Analysis of Recombinant Plant Proteins Under Oxidation. In: Mhamdi, A. (eds) Reactive Oxygen Species in Plants. Methods in Molecular Biology, vol 2526. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2469-2_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2469-2_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2468-5

  • Online ISBN: 978-1-0716-2469-2

  • eBook Packages: Springer Protocols

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