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Analysis of Ascorbate Metabolism in Arabidopsis Under High-Light Stress

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Reactive Oxygen Species in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2526))

Abstract

Ascorbate is the most abundant soluble antioxidant in plants, and its concentration is enhanced under high-light and other abiotic stresses. One of the main functions of ascorbate is the detoxification of reactive oxygen species, as ascorbate-deficient plants are highly sensitive to high-light-induced photooxidative stress. Its antioxidative role in plants is further complemented by the presence of ascorbate peroxidases, as well as enzymes that recycle ascorbate from its oxidized forms. In parallel with ascorbate biosynthesis, the expression and activity of these enzymes are enhanced by photooxidative stress. Thus, ascorbate metabolism plays a key role in photooxidative stress acclimation. Herein, the present authors’ preferred protocols for the application of high-light stress and the measurement of ascorbate and the activity of related enzymes are described.

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Acknowledgments

This work was supported by JSPS KAKENHI Grant Numbers 18K19179 (T.M), 17H03807 (T.I. and T.M.), and 19K22284 (T.I. and T.M.)

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Authors and Affiliations

  1. Department of Life Sciences, Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane, Japan

    Takanori Maruta & Takahiro Ishikawa

  2. Institute of Agricultural and Life Sciences, Academic Assembly, Shimane University, Matsue, Shimane, Japan

    Takanori Maruta & Takahiro Ishikawa

Authors
  1. Takanori Maruta
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  2. Takahiro Ishikawa
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Correspondence to Takanori Maruta .

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Editors and Affiliations

  1. VIB Center for Plant Systems Biology, Ghent University, Zwijnaarde, Belgium

    Amna Mhamdi

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Maruta, T., Ishikawa, T. (2022). Analysis of Ascorbate Metabolism in Arabidopsis Under High-Light Stress. In: Mhamdi, A. (eds) Reactive Oxygen Species in Plants. Methods in Molecular Biology, vol 2526. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2469-2_2

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  • DOI: https://doi.org/10.1007/978-1-0716-2469-2_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2468-5

  • Online ISBN: 978-1-0716-2469-2

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