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Measuring Stress-Induced Changes in Defense Phytohormones and Related Compounds

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Reactive Oxygen Species in Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2526))

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Abstract

Measuring quantitative changes in plant hormones and derivatives is crucial to understand how reactive oxygen species trigger signaling cascades to regulate stress responses. In this chapter, we describe the liquid chromatography-mass spectrometry procedure that we use to extract and quantify salicylic acid (SA), jasmonic acid (JA), and related compounds in common extracts of Arabidopsis tissue. The method can provide quantitative data on SA, SA glucosides, and JA, as well as information on oxidized and conjugated forms of these compounds and related derivatives of benzoic acid.

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Acknowledgments

We thank Grégory Mouille (IJPB, INRA Versailles) for discussion. Work in the GN laboratory is supported by the French Agence Nationale de la Recherche HIPATH project (ANR-17-CE20-0025) and by the Institut Universitaire de France (IUF).

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Authors and Affiliations

  1. Institut des Sciences des Plantes de Paris-Saclay, Unité Mixte de Recherche 8618 Centre National de la Recherche Scientifique, Orsay Cedex, France

    Caroline Lelarge-Trouverie & Graham Noctor

  2. VIB Center for Plant Systems Biology, Ghent University, Zwijnaarde, Belgium

    Amna Mhamdi

  3. Plateforme Métabolisme-Métabolome, Institut des Sciences des Plantes de Paris-Saclay, Unité Mixte de Recherche 8618 Centre National de la Recherche Scientifique, Orsay Cedex, France

    Florence Guérard

  4. Institut Universitaire de France (IUF), Paris, France

    Graham Noctor

Authors
  1. Caroline Lelarge-Trouverie
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  2. Amna Mhamdi
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  3. Florence Guérard
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  4. Graham Noctor
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Corresponding author

Correspondence to Graham Noctor .

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Editors and Affiliations

  1. VIB Center for Plant Systems Biology, Ghent University, Zwijnaarde, Belgium

    Amna Mhamdi

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Lelarge-Trouverie, C., Mhamdi, A., Guérard, F., Noctor, G. (2022). Measuring Stress-Induced Changes in Defense Phytohormones and Related Compounds. In: Mhamdi, A. (eds) Reactive Oxygen Species in Plants. Methods in Molecular Biology, vol 2526. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2469-2_16

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  • DOI: https://doi.org/10.1007/978-1-0716-2469-2_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2468-5

  • Online ISBN: 978-1-0716-2469-2

  • eBook Packages: Springer Protocols

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