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Genetic analysis and molecular mapping of a new powdery mildew resistant gene Pm46 in common wheat

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Abstract

Powdery mildew (PM), caused by Blumeria graminis f. sp. tritici (Bgt), has become a serious disease and caused severe yield losses in the wheat production worldwide. Resistance gene(s) in wheat cultivars can be quickly overcome by newly evolved pathogen races when these genes are employed for long time or in a large area. It is urgent to search for new sources of resistance to be used in wheat breeding. Tabasco is a German resistant cultivar and a new source of resistance gene(s) to PM. An F2 population was developed from a cross between Tabasco and a Chinese susceptible cultivar Ningnuo 1. Infection types in 472 F2 plants and 436 F2–3 families were evaluated by inoculating plants with isolate Bgt19. Results showed that a single dominant gene, designed Pm46, controlled powdery mildew resistance in Tabasco. This gene was located to the short arm of chromosome 5D (5DS) and flanked by simple sequence repeat markers Xgwm205 and Xcfd81 at 18.9 cM apart. Because another resistance gene Pm2 was also located on 5DS, 15 Bgt isolates were used to inoculate Tabasco and Ulka/8*Cc (Pm2 carrier). The results showed that Tabasco was highly resistant to all of the 15 isolates tested, while Ulka/8*Cc was susceptible to 4 of the isolates, suggesting that Tabasco may carry resistant gene(s) different from Pm2 gene in Ulka/8*Cc. To test the allelism between Pm46 and Pm2, an F2 population between Tabasco and Ulka/8*Cc was developed. Isolate Bgt2, avirulent to both parents, was used to evaluate the F2 population and two susceptible plants were identified from 536 progenies with F2 plants. This result indicated that Pm46 is not allelic to Pm2. Therefore, Pm46 is a new gene for PM resistance identified in this study.

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Acknowledgments

We are grateful to Professor Zhengqiang Ma, Nanjing Agricultural University, for providing Bgt isolates and to Hongqi Ma and Bisheng Fu for helping in the experiment, and Dr. Guihua Bai, USDA/ARS in Kansas State University, Manhattan KS for suggestion in improving the manuscript. This work was supported by the National Science and Technology Support Program in China (2006BAD13B02-11), Jiangsu Provincial Foundation of Agricultural Scientific Innovation (cx (09)633) in China.

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Authors and Affiliations

  1. Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    Haidong Gao, Fangfang Zhu, Yanjie Jiang, Jizhong Wu, Wei Yan, Qiaofeng Zhang & Shibin Cai

  2. Nanjing Agricultural University, Nanjing, 210095, China

    Haidong Gao & Fangfang Zhu

  3. Strube Research GmbH & Co. KG, Hauptstraße 1, 38387, Söllingen, Germany

    Andreas Jacobi

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  1. Haidong Gao
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Correspondence to Shibin Cai.

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Communicated by C. Hackett.

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Gao, H., Zhu, F., Jiang, Y. et al. Genetic analysis and molecular mapping of a new powdery mildew resistant gene Pm46 in common wheat. Theor Appl Genet 125, 967–973 (2012). https://doi.org/10.1007/s00122-012-1886-7

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  • DOI: https://doi.org/10.1007/s00122-012-1886-7

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