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Identification and mapping of a new powdery mildew resistance allele in the Chinese wheat landrace Hongyoumai

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Abstract

Powdery mildew (Pm) caused by Blumeria graminis f. sp. tritici (Bgt) is one of the world’s major wheat diseases and results in large grain yield losses. Discovery and utilization of Pm resistance genes constitute the most common strategy for wheat Pm control. Hongyoumai, a wheat landrace from Henan Province in China, has excellent resistance to infection by Bgt. In order to identify the basis of such Pm resistance, a segregating population was submitted to genetic analysis, which showed that Pm resistance in Hongyoumai was conferred by a single recessive resistance gene. This gene was temporarily named pmHYM. Molecular marker analysis, chromosomal location, resistance spectrum analysis, and an allelism test showed that pmHYM was located on the long arm of chromosome 7B (7BL), most likely representing a new recessive resistance gene allelic with Pm5e and mlXBD. By using 90-kb single-nucleotide polymorphism sequences (SNP) in the BLASTn analysis against the wheat 7BL genome sequence, 12 new simple sequence repeat (SSR) markers linked with pmHYM were developed to map pmHYM co-segregating with the marker Xmp1207 and between markers Xmp925 and Xmp1158, at genetic distances of 2.8 and 2.7 cM, respectively. In addition, physical mapping of the markers linked with pmHYM using Chinese Spring deletion lines indicated a location in the 0.86–1.00 bin of 7BL.

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Acknowledgements

This project was supported by the National Key Research and Development Program of China (2016YFD0100102-3), the National Natural Science Foundation of China (No. 31501313), the Natural Science Foundation of Jiangsu Province (Grant no. BK20140757), and the Jiangsu Provincial Foundation of Agricultural Scientific Innovation (Grant no. cx [14]5006) in China. We are grateful to Thomas S. Cox, the Land Institute, USA, for improving the manuscript.

Authorʼs contribution

B. Fu, J. Wu, and S. Cai designed the study; B. Fu collected the plant materials and generated the populations; B. Fu, Z Zhang, Q Zhang, and X Wu performed the experiments; B. Fu and Z. Zhang analyzed the data; B. Fu wrote the manuscript.

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  1. Bisheng Fu and Zhiliang Zhang contributed equally to this article.

Authors and Affiliations

  1. Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences/The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing, 210014, People’s Republic of China

    Bisheng Fu, Zhiliang Zhang, Qiaofeng Zhang, Xiaoyou Wu, Jizhong Wu & Shibin Cai

  2. Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Zhiliang Zhang

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  1. Bisheng Fu
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Correspondence to Jizhong Wu.

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The authors declare that these experiments comply with the current laws of China and no conflicts of interest.

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Key message

The new powdery mildew resistance allele pmHYM, very likely at the Pm5 locus, was identified and mapped in the Chinese wheat landrace Hongyoumai.

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Supplementary Figure 1

Comparison of previously reported genetic linkage maps of various powdery mildew resistance genes at or near the Pm5 locus on chromosome 7BL. Bold markers are common to the four genes. The vertical arrow indicates the direction toward the telomere. (50 kb).

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Fu, B., Zhang, Z., Zhang, Q. et al. Identification and mapping of a new powdery mildew resistance allele in the Chinese wheat landrace Hongyoumai. Mol Breeding 37, 133 (2017). https://doi.org/10.1007/s11032-017-0728-3

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