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Pm34: a new powdery mildew resistance gene transferred from Aegilops tauschii Coss. to common wheat (Triticum aestivum L.)

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Abstract

Powdery mildew is a major fungal disease in wheat growing areas worldwide. A novel source of resistance to wheat powdery mildew present in the germplasm line NC97BGTD7 was genetically characterized as a monogenic trait in greenhouse and field trials using F2 derived lines from a NC97BGTD7 X Saluda cross. Microsatellite markers were used to map and tag this resistance gene, now designated Pm34. Three co-dominant microsatellite markers linked to Pm34 were identified and their most likely order was established as: Xbarc177-5D, 5.4cM, Pm34, 2.6cM, Xbarc144-5D, 14cM, Xgwm272-5D. These microsatellite markers were previously mapped to the long arm of the 5D chromosome and their positions were confirmed using Chinese Spring nullitetrasomic Nulli5D-tetra5A and ditelosomic Dt5DL lines. Pm2, the only other known Pm gene on chromosome 5D, has been mapped to the short arm and its specificity is different from that of Pm34.

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References

  • Anderson JA, Ogihara Y, Sorrells ME, Tanksley SD (1992) Development of a chromosome arm map for wheat based on RFLP markers. Theor Appl Genet 83:1035–1043

    CAS  Google Scholar 

  • Bougot Y, Lemoine J, Pavoine MT, Barloy D, Doussinault G (2002) Identification of a microsatellite associated with Pm3 resistance alleles to powdery mildew in wheat. Plant Breed 121:325–329

    Article  CAS  Google Scholar 

  • Chen XM, Luo YH, Xia XC, Xia LQ, Chen X, Ren ZL, He ZH, Jia JZ (2005) Chromosomal location of powdery mildew resistance gene Pm16 in wheat using SSR marker analysis. Plant Breed 124:225–228

    Article  CAS  Google Scholar 

  • Everts K, Leath S, Finney PL (2001) Impact of powdery mildew and leaf rust on milling and baking quality of soft red winter wheat. Plant Dis 85:423–429

    Google Scholar 

  • Gupta PK, Varshney RK, Sharma PC, Ramesh B (1999) Molecular markers and their applications in wheat breeding. Plant Breed 118:369–390

    Article  CAS  Google Scholar 

  • Gupta PK, Baylan HS, Edwards KJ, Isaac P, Korzun V, Röder MS, Gautier MF, Joudrier P, Schlatter AR, Dubcovscy J, De la Pena RC, Khairalla M, Penner G, Hayden MJ, Sharp P, Keller B, Wang RCC, Hardouin JP, Jack P, Leroy P (2002) Genetic mapping of 66 microsatellite (SSR) loci in bread wheat. Theor Appl Genet 105:413–422

    Article  PubMed  CAS  Google Scholar 

  • Huang XQ, Hsam SLK, Zeller FJ, Wenzel G, Mohler V (2000) Molecular mapping of wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding. Theor Appl Genet 101:407–414

    Article  CAS  Google Scholar 

  • Huang XQ, Wang LX, Xu MX, Röder MS (2003) Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat (Triticum aestivum L.). Theor Appl Genet 106:858–865

    PubMed  CAS  Google Scholar 

  • Huang XQ, Hsam SLK, Mohler V, Röder MS, Zeller F (2004) Genetic mapping of three alleles at the Pm3 locus conferring powdery mildew resistance in common wheat (Triticum aestivum L.). Genome 47:1130–1136

    Article  PubMed  CAS  Google Scholar 

  • Huang XQ, Röder MS (2004) Molecular mapping of powdery mildew resistance genes in wheat: a review. Euphytica 137:203–223

    Article  CAS  Google Scholar 

  • Hsam SLK, Zeller FJ (2002) Breeding for powdery mildew resistance in common wheat (Triticum aestivum L.) In: Berlanger RR, Bushnell WR, Dik AJ, Carver DL (eds) The powdery mildews: a comprehensive treatise. American Phytopathological Society MN, pp 219–238

  • Järve K, Peusha HO, Tsybalova J, Tamm S, Devos KM, Enno TM (2000) Chromosomal location of a Triticum timopheevi derived powdery mildew resistance gene transferred to common wheat. Genome 43:377–381

    Article  PubMed  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Langridge P, Lagudah ES, Holton TA, Appels R, Sharp PJ, Chalmers KJ (2001) Trends in genetics and genome analyses in wheat: a review. Aust J Agric Res 52:1043–1077

    Article  CAS  Google Scholar 

  • Large EC (1954) Growth stages in cereals. Illustrations of the Feeke’s scale. Plant Pathol 3:129

    Google Scholar 

  • Leath S, Heun M (1990) Identification of powdery mildew resistance genes in cultivars of soft red winter wheat. Plant Dis 74:747–752

    Google Scholar 

  • Lincoln SE, Daly MJ, Lander ES (1993) Constructing linkage maps with MAPMAKER/Exp Version 3.0. A tutorial reference manual, 3rd edn. Whitehead Institute for Medical Res., Cambridge, MA

  • Lipps PE, Madden V (1989) Assesment of methods of determining powdery mildew severity in relation to grain yield of winter wheat cultivars in Ohio. Phytopathology 79:462–470

    Google Scholar 

  • Liu J, Liu D, Tao W, Li W, Wang S, Chen P, Cheng S, Gao D (2000) Molecular marker-facilitated pyramiding of different genes for powdery mildew resistance in wheat. Plant Breed 119:21–24

    Article  CAS  Google Scholar 

  • Liu ZY, Sun QX, Ni ZF, Nevo E, Yang TM (2002) Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer. Euphytica 123:21–29

    Article  CAS  Google Scholar 

  • Lutz J, Hsam SLK, Limpert E, Zeller FJ (1994) Powdery mildew resistance genes in Aegilops tauschii Coss. and synthetic hexaploid wheats. Genet Resour Crop Evol 41:151–158

    Article  Google Scholar 

  • Ma ZQ, Sorrels ME, Tanksley SD (1994) RFLP markers linked to powdery mildew resistance genes Pm1, Pm2, Pm3 and Pm4 in wheat. Genome 37:871–875

    CAS  PubMed  Google Scholar 

  • Ma ZQ, Wei JB, Chen SH (2004) PCR based markers for the powdery mildew resistance gene Pm4a in wheat. Theor Appl Genet 109:140–145

    Article  PubMed  CAS  Google Scholar 

  • McIntosh RA, Baker EP (1970) Cytogenetic studies in wheat IV Chromosomal location and linkage studies involving the Pm2 locus for powdery mildew resistance. Euphytica 19:71–77

    Article  Google Scholar 

  • McDonald BA, Linde C (2002) Pathogen population genetics, evolutionary potential and durable resistance. Annu Rev Phytopathol 40:349–379

    Article  PubMed  CAS  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating population. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed  CAS  Google Scholar 

  • Murphy JP, Leath S, Huynh D, Navarro RA, Shi A (1998) Registration of NC96BGTD1, NC96BGTD2 and NC96BGTD3 wheat germplasm resistant to powdery mildew. Crop Sci 38:570–571

    Article  Google Scholar 

  • Murphy JP, Leath S, Huynh D, Navarro RA, Shi A (1999a) Registration of NC96BGTA4, NC96BGTA5 and NC96BGTA6 wheat germplasm. Crop Sci 39:883–884

    Article  Google Scholar 

  • Murphy JP, Leath S, Huynh D, Navarro RA, Shi A (1999b) Registration of NC97BGTD7 and NC97BGTD8 wheat germplasms resistant to powdery mildew. Crop Sci 39:884–885

    Article  Google Scholar 

  • Murphy JP, Navarro RA, Leath S (2002) Registration of NC99BGTAG11 wheat germplasm resistant to powdery mildew. Crop Sci 42:1382

    Article  Google Scholar 

  • Navarro RA, Murphy JP, Leath S, Shi A (2000) Registration of NC97BGTAB9 and NC97BGTAB10 wheat germplasm lines resistant to powdery mildew. Crop Sci 40:1508–1509

    Google Scholar 

  • Niewoehner AS, Leath S (1998) Virulence of Blumeria graminis f. sp. tritici on winter wheat in the eastern United States. Plant Dis 82:64–68

    Google Scholar 

  • Paillard S, Schnurbusch T, Winzeler M, Messmer M, Sourdille P, Abderhalden O, Keller B, Schachermayr G (2003) An integrative genetic linkage map of wheat. Theor Appl Genet 107:1235–1242

    Article  PubMed  CAS  Google Scholar 

  • Pestova E, Ganal MW, Röder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43:689–697

    Article  Google Scholar 

  • Plaschke JB, Börner A, Wendehake K, Ganal MW, Röder MS (1996) The use of aneuploids for the chromosomal assignment of microsatellite loci. Euphytica 89:33–40

    Article  CAS  Google Scholar 

  • Qiu YC, Zhou RH, Kong XY, Zhang SS, Jia JZ (2005) Microsatellite mapping of a Triticum urartu Tum. derived powdery mildew resistance gene transferred to common wheat (Triticum aestivum L.). Theor Appl Genet 111:1524–1531

    Article  PubMed  CAS  Google Scholar 

  • Rampling LR, Harker N, Shariflou MR, Morell MK (2001) Detection and analysis systems for microsatellite markers in wheat. Aust J Agric Res 52:1131–1141

    Article  CAS  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Schuelke M (2000) An economic method for fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234

    Article  PubMed  CAS  Google Scholar 

  • Singrün CH, Hsam SL, Zeller FJ, Mohler V (2003) Powdery mildew resistance gene Pm22 is a member of the complex Pm1 locus in common wheat (Triticum aestivum L). Theor Appl Genet 106:1420–1424

    PubMed  Google Scholar 

  • Sommers DJ, Isaac P, Edward K (2004) A high density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  CAS  Google Scholar 

  • Srnić G, Murphy JP, Lyerly JH, Leath S, Marshall DS (2005) Inheritance and chromosomal assignment of powdery mildew resistance genes in two winter wheat germplasm lines. Crop Sci 45:1578–1586

    Article  CAS  Google Scholar 

  • Starling TM, Roane CW, Camper HM (1986) Registration of Saluda wheat. Crop Sci 26:200

    Article  Google Scholar 

  • Stein N, Herren G, Keller B (2001) A new DNA extraction method for high throughput marker analysis in a large genome species such as Triticum aestivum L. Plant Breed 120:354–356

    Article  CAS  Google Scholar 

  • Stephenson P, Bryan G, Kirby J, Collins A, Devos K, Busso C, Gale M (1998) Fifty new microsatellite loci for the wheat genetic map. Theor Appl Genet 100:564–568

    Google Scholar 

  • Weisz R (2000) Small grain production guide 2000–2001. North Carolina Coop. Ext. Serv., Raleigh, NC

    Google Scholar 

  • Xie CJ, Sun QX, Ni ZF, Yang ZM, Nevo E, Fahima T (2003) Chromosomal location of a Triticum dicoccoides-derived powdery mildew resistance gene in common wheat by using microsatellite markers. Theor Appl Genet 106:341–345

    PubMed  CAS  Google Scholar 

  • Zhu ZD, Zhou RH, Kong XY, Dong YC, Jia JZ (2005) Microsatellite markers linked to two genes conferring resistance to powdery mildew in common wheat introgressed from Triticum carthlicum accession PS5. Genome 48:585–590

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors thank Rene Navarro and David Wooten for their help with field work, Jeannette Lyerly for her technical assistance and Lynda Whitcher for providing the powdery mildew isolate. We gratefully acknowledge Prof. Bob McIntosh for some excellent additions to the final draft of this manuscript.

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Authors and Affiliations

  1. Department of Crop Science, North Carolina State University, Box 7629, Raleigh, NC, 27695, USA

    L. M. Miranda & J. P. Murphy

  2. United States Department of Agriculture—Agricultural Research Service, North Carolina State University, Box 7619, Raleigh, NC, 27695, USA

    D. Marshall

  3. Department of Plant Pathology, North Carolina State University, Box 7619, Raleigh, NC, 27695, USA

    S. Leath

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  1. L. M. Miranda
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  2. J. P. Murphy
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  3. D. Marshall
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  4. S. Leath
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Correspondence to J. P. Murphy.

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Communicated by D. A. Lightfoot.

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Miranda, L.M., Murphy, J.P., Marshall, D. et al. Pm34: a new powdery mildew resistance gene transferred from Aegilops tauschii Coss. to common wheat (Triticum aestivum L.). Theor Appl Genet 113, 1497–1504 (2006). https://doi.org/10.1007/s00122-006-0397-9

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  • DOI: https://doi.org/10.1007/s00122-006-0397-9

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