Abstract
A single gene controlling powdery mildew resistance was identified in the North Carolina germplasm line NC96BGTD3 (NCD3) using genetic analysis of F2 derived lines from a NCD3 X Saluda cross. Microsatellite markers linked to this Pm gene were identified and their most likely order was Xcfd7, 10.3 cM, Xgdm43, 8.6 cM, Xcfd26, 11.9 cM, Pm gene. These markers and the Pm gene were assigned to chromosome 5DL by means of Chinese Spring Nullitetrasomic (Nulli5D-tetra5A) and ditelosomic (Dt5DL) lines. A detached leaf test showed a distinctive disease reaction to six pathogen isolates among the NCD3 Pm gene, Pm2 (5DS) and Pm34 (5DL). An allelism test showed independence between Pm34 and the NCD3 Pm gene. Together, the tests provided strong evidence for the presence of a novel Pm gene in NCD3, and this gene was designated Pm35.
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Acknowledgments
The authors thank Dr. Robert McIntosh for his comprehensive review of this manuscript. We would also like to acknowledge Rene Navarro and David Wooten for their excellent help with the field experiment and Jeanette Lyerly for her technical assistance.
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Miranda, L.M., Murphy, J.P., Marshall, D. et al. Chromosomal location of Pm35, a novel Aegilops tauschii derived powdery mildew resistance gene introgressed into common wheat (Triticum aestivum L.). Theor Appl Genet 114, 1451–1456 (2007). https://doi.org/10.1007/s00122-007-0530-4
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DOI: https://doi.org/10.1007/s00122-007-0530-4