Skip to main content
SpringerLink
Log in

Genetic mapping of two powdery mildew resistance genes in einkorn (Triticum monococcum L.) accessions

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Powdery mildew is a severe foliar disease for wheat and could cause great yield loss in epidemic years. To explore new powdery mildew resistance genes, two einkorn accessions including TA2033 and M80, both resistant to this disease, were studied for the inheritance of resistance. Each accession possessed a single but different dominant resistance gene that was designated as Mlm2033 and Mlm80, respectively. Marker mapping indicated that they are both linked to Xgwm344 on the long arm of chromosome 7A. To establish their genetic relationship with Pm1 on 7AL, five RFLP markers previously reported to co-segregate with Pm1a were converted to STS markers. Three of them detected polymorphism between the mapping parents and were mapped close to Mlm2033 or Mlm80 or both. Xmag2185, the locus determined by the STS marker derived from PSR680, one of the RFLP markers, was placed less than 2 cM away from them. The allelism test indicated that Mlm2033 and Mlm80 are likely allelic to each other. In addition, through comparative and EST mapping, more markers linked to these two genes were identified. The high density mapping of Mlm2033 and Mlm80 will contribute to map-based cloning of the Pm1 locus. The markers for both genes will also facilitate their transfer to wheat.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Clarkson JDS (2000) Virulence survey report for wheat powdery mildew in Europe, 1996–1998. http://www.crpmb.org/2000/1204clarkson

  • Duan S, Xu Y, Wu X (2002) Research progress of pathogen virulence, resistance genes and resistance breeding of wheat powdery mildew (in Chinese). J Triticeae Crops 22:83–86

    Google Scholar 

  • Dubcovsky J, Dvorák J (1995) Ribosomal RNA multigene loci: nomads of the Triticeae genomes. Genetics 140:1367–1377

    PubMed  CAS  Google Scholar 

  • Dubcovsky J, Luo MC, Dvorák J (1995) Differentiation between homoeologous chromosomes 1A of wheat and lAm of Triticum monococcum and its recognition by the wheat Phl locus. Proc Natl Acad Sci USA 92:6645–6649

    Article  PubMed  CAS  Google Scholar 

  • Guyomarc’h H, Sourdille P, Edwards KJ, Bernard M (2002) Studies of the transferability of microsatellites derived from Triticum tauschii to hexaploid wheat and to diploid related species using amplification, hybridization and sequence comparisons. Theor Appl Genet 105:736–744

    Article  PubMed  CAS  Google Scholar 

  • Guyot R, Yahiaoui N, Feuillet C, Keller B (2004) In silico comparative analysis reveals a mosaic conservation of genes within a novel collinear region in wheat chromosomes 1AS and rice chromosome 5S. Funct Integr Genomics 4:47–58

    Article  PubMed  CAS  Google Scholar 

  • Hossain KG, Kalavacharla V, Lazo GR, Hegstad J, Wentz MJ, Kianian PMA, Simons K, Gehlhar S, Rust JL, Syamala RR, Obeori K, Bhamidimarri S, Karunadharma P, Chao S, Anderson OD, Qi LL, Echalier B, Gill BS, Linkiewicz AM, Ratnasiri A, Dubcovsky J, Akhunov ED, Dvorák J, Miftahudin, Ross K, Gustafson JP, Radhawa HS, Dilbirligi M, Gill KS, Peng JH, Lapitan NLV, Greene RA, Bermudez-Kandianis CE, Sorrells ME, Feril O, Pathan MS, Nguyen HT, Gonzalez-Hernandez JL, Conley EJ, Anderson JA, Choi DW, Fenton D, Close TJ, McGuire PE, Qualset CO, Kianian SF (2004) A chromosome bin map of 2148 expressed sequence tag loci of wheat homoeologous group 7. Genetics 168:687–699

    Article  PubMed  CAS  Google Scholar 

  • Hsam SLK, Huang XQ, Ernst F, Hartl L, Zeller FJ (1998) Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.). 5. Alleles at the Pm1 locus. Theor Appl Genet 96:1129–1134

    Article  CAS  Google Scholar 

  • Kilian A, Chen J, Han F, Steffenson B, Kleinhofs A (1997) Towards map-based cloning of the barley stem rust resistance genes Rpg1 and rpg4 using rice as an intergenomic cloning vehicle. Plant Mol Biol 35:187–195

    Article  PubMed  CAS  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daley M, Lincoln S, Newburg L (1987) Mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Liu J, Liu D, Tao W, Li W, Wang S, Chen P, Cheng S, Gao D (2000) Molecular marker-facilitated pyramiding of different genes for powdery mildew resistance in wheat. Plant Breed 119:21–24

    Article  CAS  Google Scholar 

  • Ma ZQ, Sorrells ME, Tanksley SD (1994) RELP marker linked to powdery mildew resistance genes Pm1, Pm2, Pm3, and Pm4 in wheat. Genome 37:871–875

    CAS  PubMed  Google Scholar 

  • McDonald BA, Linde C (2002) Pathogen population genetics, evolution potential and durable resistance. Annu Rev Phytopathol 40:349–79

    Article  PubMed  CAS  Google Scholar 

  • McIntosh RA, Yamazaki Y, Devos KM, Dubcovsky J, Rogers WJ, Appels R (2003) Catalogue of gene symbols for wheat. In: Pogna NE, Romano M, Pogna EA, Galterio G (eds) Proceedings of the 10th international wheat genetic symposium, vol 4, pp 1–34

  • McIntosh RA, Devos KM, Dubcovsky J, Rogers WJ (2004) Catalogue of gene symbols for wheat: 2004(suppl). http://www.wheat.pw.usda.gov/GG2/pubs.shtml

  • McIntosh RA, Devos KM, Dubcovsky J, Morris CF, Appels, Anderson OD (2005) Catalogue of gene symbols for wheat: 2005(suppl). http://www.wheat.pw.usda.gov/GG2/pubs.shtml

  • Neu C, Stein N, Keller B (2002) Genetic mapping of the Lr20-Pm1 resistance locus reveals suppressed recombination on chromosome arm 7AL in hexaploid wheat. Genome 45:737–744

    Article  PubMed  CAS  Google Scholar 

  • Niewoehner AS, Leath S (1998) Virulence of Blumeria graminis f. sp. tritici on winter wheat in the eastern United States. Plant Dis 82:64–68

    Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Santos FR, Pena SDJ, Epplen JT (1993) Genetic and population study of a Y-linked tetranucleotide repeat DNA polymorphism. Hum Genet 90:655–656

    Article  PubMed  CAS  Google Scholar 

  • Schneider DM, Heun M, Fischbeck G (1991) Inheritance of the powdery mildew resistance gene Pm9 in relation to Pm1 and Pm2 of wheat. Plant Breed 107:161–164

    Article  Google Scholar 

  • Sears ER, Briggle LW (1969) Mapping the gene Pm1 for resistance to Erysiphe graminis f.sp. tritici on chromosome 7A of wheat. Crop Sci 9:96–97

    Article  Google Scholar 

  • Singrün C, Hsam SLK, Hartl L, Zeller FJ, Mohler V (2003) Powdery mildew resistance gene Pm22 in cultivar Virest is a member of the complex Pm1 locus in common wheat (Triticum aestivum L. em Thell.). Theor Appl Genet 106:1420–1424

    PubMed  Google Scholar 

  • Singrün C, Hsam SLK, Zeller FJ, Wenzel G, Mohler V (2004) Localization of a novel recessive powdery mildew resistance gene from common wheat line RD30 in the terminal region of chromosome 7AL. Theor Appl Genet 109:210–214

    Article  PubMed  CAS  Google Scholar 

  • Sorrells ME, La Rota M, Bermudez-Kandianis CE, Greene RA, Kantety R, Munkvold JD, Miftahudin, Mahmoud A, Ma X, Gustafson PJ, Qi L, Echalier B, Gill BS, Matthews DE, Lazo GR, Chao S, Anderson OD, Edwards H, Linkiewicz AM, Dubcovsky J, Akhunov ED, Dvorak J, Zhang D, Nguyen HT, Peng J, Lapitan NLV, Gonzalez-Hernandez JL, Anderson JA, Hossain K, Kalavacharla V, Kianian SF, Choi DW, Close TJ, Dilbirligi M, Gill KS, Steber C, Walker-Simmons MK, McGuire PE, Qualset CO (2003) Comparative DNA sequence analysis of wheat and rice genomes. Genome Res 13:1818–1827

    PubMed  CAS  Google Scholar 

  • Sourdille P, Cadalen T, Guyomarc’h H, Snape JW, Perretant MR, Charmet G, Boeuf C, Bernard S, Bernard M (2003) An update of the Courtot x Chinese Spring intervarietal molecular marker linkage map for the QTL detection of agronomic traits in wheat. Theor Appl Genet 106:530–538

    PubMed  CAS  Google Scholar 

  • Srnic G, Murphy JP, Lyerly JH, Leath S, Marshall DS (2005) Inheritance and chromosomal assignment of powdery mildew resistance genes in two winter wheat germplasm lines. Crop Sci 45:1578–1586

    Article  CAS  Google Scholar 

  • Tanksley SD, McCouch SR (1997) Seed banks and molecular maps: unlocking genetic potential from the wild. Science 277:1063–1066

    Article  PubMed  CAS  Google Scholar 

  • Yahiaoui N, Srichumpa P, Dudler Robert, Keller B (2004) Genome analysis at different ploidy levels allows cloning of the powdery mildew resistance gene Pm3b form hexaploid wheat. Plant J 37:528–538

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This project was partially supported by NSFC Fund (30025030), and MOE program for outstanding young faculty.

Author information

Authors and Affiliations

  1. The Applied Plant Genomics Lab and National Key Lab for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Jiangsu, People’s Republic of China

    Guoqi Yao, Jiangli Zhang, Lili Yang, Hongxing Xu, Yumei Jiang, Li Xiong, Caiqin Zhang, Zhengzhi Zhang & Zhengqiang Ma

  2. Department of Plant Breeding, Cornell University, Ithaca, NY, 14853, USA

    Mark E. Sorrells

Authors
  1. Guoqi Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jiangli Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lili Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hongxing Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yumei Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Li Xiong
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Caiqin Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Zhengzhi Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Zhengqiang Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Mark E. Sorrells
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhengqiang Ma.

Additional information

Communicated by F. Ordon.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yao, G., Zhang, J., Yang, L. et al. Genetic mapping of two powdery mildew resistance genes in einkorn (Triticum monococcum L.) accessions. Theor Appl Genet 114, 351–358 (2007). https://doi.org/10.1007/s00122-006-0438-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-006-0438-4

Keywords

Search

Navigation