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Identification and genetic mapping of pm42, a new recessive wheat powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides)

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Abstract

Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most important wheat diseases worldwide in areas with cool or maritime climates. Wild emmer (Triticum turgidum var. dicoccoides) is an important potential donor of disease resistances and other traits for common wheat improvement. A powdery mildew resistance gene was transferred from wild emmer accession G-303-1M to susceptible common wheat by crossing and backcrossing, resulting in inbred line P63 (Yanda1817/G-303-1 M//3*Jing411, BC2F6). Genetic analysis of an F2 population and the F2:3 families developed from a cross of P63 and a susceptible common wheat line Xuezao showed that the powdery mildew resistance in P63 was controlled by a single recessive gene. Molecular markers and bulked segregant analysis were used to characterize and map the powdery mildew resistance gene. Nine genomic SSR markers (Xbarc7, Xbarc55, Xgwm148, Xgwm257, Xwmc35, Xwmc154, Xwmc257, Xwmc382, Xwmc477), five AFLP-derived SCAR markers (XcauG3, XcauG6, XcauG10, XcauG20, XcauG22), three EST–STS markers (BQ160080, BQ160588, BF146221) and one RFLP-derived STS marker (Xcau516) were linked to the resistance gene, designated pm42, in P63. pm42 was physically mapped on chromosome 2BS bin 0.75–0.84 using Chinese Spring nullisomic-tetrasomic, ditelosomic and deletion lines, and was estimated to be more than 30 cM proximal to Xcau516, a RFLP-derived STS marker that co-segregated with the wild emmer-derived Pm26 which should be physically located in 2BS distal bin 0.84–1.00. pm42 was highly effective against 18 of 21 differential Chinese isolates of B. graminis f. sp. tritici. The closely linked molecular markers will enable the rapid transfer of pm42 to wheat breeding populations thus adding to their genetic diversity.

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Acknowledgments

We are grateful to Dr. R. McIntosh for improving the manuscript. This work was financially supported by the National Fund for Distinguished Young Scholars (30425039), National Natural Science Foundation of China (30571151, 30771341), Beijing Natural Science Foundation (6061003), and the State High Tech Programs (2006AA100102, 2006AA10Z1E9, 2006AA10Z1C4, 2006AA10A104 and 2006BAD01A02), State Transgenic Project (2008ZX08009-002), the Program of Introducing Talents of Discipline to Universities (111-2-03), and the Program for Changjiang Scholars and Innovative Research Team in University. The gift of aneuploids and deletion stocks from Prof. B·S. Gill and Mr. W.J. Raupp, Wheat Genetic Resource Centre, Kansas State University, USA, and of RFLP probe WG516 by Drs. D. Benscher and M. Sorrells, Cornell University, NY, USA, are gratefully acknowledged.

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Authors and Affiliations

  1. State Key Laboratory for Agrobiotechnology, China Agricultural University, 100193, Beijing, People’s Republic of China

    Qixin Sun & Zhiyong Liu

  2. Department of Plant Genetics and Breeding, China Agricultural University, 100193, Beijing, People’s Republic of China

    Wei Hua, Ziji Liu, Jie Zhu, Chaojie Xie, Tsomin Yang, Qixin Sun & Zhiyong Liu

  3. Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, 100193, Beijing, People’s Republic of China

    Qixin Sun & Zhiyong Liu

  4. Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, 100193, Beijing, People’s Republic of China

    Qixin Sun & Zhiyong Liu

  5. Key Laboratory of Crop Heterosis Research and Utilization, Ministry of Education, China Agricultural University, 100193, Beijing, People’s Republic of China

    Qixin Sun & Zhiyong Liu

  6. Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, 100094, Beijing, People’s Republic of China

    Yilin Zhou & Xiayu Duan

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  1. Wei Hua
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  2. Ziji Liu
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  3. Jie Zhu
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  8. Qixin Sun
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  9. Zhiyong Liu
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Corresponding authors

Correspondence to Qixin Sun or Zhiyong Liu.

Additional information

Communicated by B. Keller.

W. Hua, Z. Liu, and J. Zhu contributed equally to this work.

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Hua, W., Liu, Z., Zhu, J. et al. Identification and genetic mapping of pm42, a new recessive wheat powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides). Theor Appl Genet 119, 223–230 (2009). https://doi.org/10.1007/s00122-009-1031-4

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  • DOI: https://doi.org/10.1007/s00122-009-1031-4

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