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Genetic and comparative genomics mapping reveals that a powdery mildew resistance gene Ml3D232 originating from wild emmer co-segregates with an NBS-LRR analog in common wheat (Triticum aestivum L.)

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Abstract

Powdery mildew caused by Blumeria graminis f. sp. tritici is one of the most important wheat diseases worldwide and breeding for resistance using diversified disease resistance genes is the most promising approach to prevent outbreaks of powdery mildew. A powdery mildew resistance gene, originating from wild emmer wheat (Triticum turgidum var. dicoccoides) accessions collected from Israel, has been transferred into the hexaploid wheat line 3D232 through crossing and backcrossing. Inoculation results with 21 B. graminis f. sp. tritici races indicated that 3D232 is resistant to all of the powdery mildew isolates tested. Genetic analyses of 3D232 using an F2 segregating population and F3 families indicated that a single dominant gene, Ml3D232, confers resistance in the host seedling stage. By applying molecular markers and bulked segregant analysis (BSA), we have identified polymorphic simple sequence repeats (SSR), expressed sequence tags (EST) and derived sequence tagged site (STS) markers to determine that the Ml3D232 is located on chromosome 5BL bin 0.59–0.76. Comparative genetic analyses using mapped EST markers and genome sequences of rice and Brachypodium established co-linearity of the Ml3D232 genomic region with a 1.4 Mb genomic region on Brachypodium distachyon chromosome 4, and a 1.2 Mb contig located on the Oryza sativa chromosome 9. Our comparative approach enabled us to develop new EST–STS markers and to delimit the genomic region carrying Ml3D232 to a 0.8 cM segment that is collinear with a 558 kb region on B. distachyon. Eight EST markers, including an NBS-LRR analog, co-segregated with Ml3D232 to provide a target site for fine genetic mapping, chromosome landing and map-based cloning of the powdery mildew resistance gene. This newly developed common wheat germplasm provides broad-spectrum resistance to powdery mildew and a valuable resource for wheat breeding programs.

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Acknowledgments

The authors are grateful to Prof. Andrew Jackson, UC Berkeley, for improving the manuscript. This work was financially supported by the National Fund for Distinguished Young Scholars (30425039), National Natural Science Foundation of China (30771341, 30971780), MOA project 2009ZX08009-048B, the State High Tech Programs (2006AA100102, 2006AA10Z1E9, 2006AA10Z1C4, 2006BAD01A02), the Program of Introducing Talents of Discipline to Universities (111-2-03), and the Program for Changjiang Scholars and Innovative Research Teams in Universities. The aneuploid and deletion stocks gifts from Prof. B. S. Gill and Mr. W. J. Raupp, Wheat Genetics Resource Centre, Kansas State University, USA are gratefully acknowledged.

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Authors and Affiliations

  1. State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, People’s Republic of China

    Hongtao Zhang, Haiying Guan, Jingting Li, Jie Zhu, Chaojie Xie, Tsomin Yang, Qixin Sun & Zhiyong Liu

  2. Key Laboratory of Crop Genomics and Genetic Improvement, China Agricultural University, Ministry of Agriculture, Beijing, 100193, People’s Republic of China

    Hongtao Zhang, Haiying Guan, Jingting Li, Jie Zhu, Chaojie Xie, Tsomin Yang, Qixin Sun & Zhiyong Liu

  3. Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, People’s Republic of China

    Hongtao Zhang, Haiying Guan, Jingting Li, Jie Zhu, Chaojie Xie, Tsomin Yang, Qixin Sun & Zhiyong Liu

  4. Key Laboratory of Crop Heterosis Research and Utilization, China Agricultural University, Ministry of Education, Beijing, 100193, People’s Republic of China

    Hongtao Zhang, Haiying Guan, Jingting Li, Jie Zhu, Chaojie Xie, Tsomin Yang, Qixin Sun & Zhiyong Liu

  5. Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing, 100094, People’s Republic of China

    Yilin Zhou & Xiayu Duan

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  1. Hongtao Zhang
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  2. Haiying Guan
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  6. Yilin Zhou
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  8. Tsomin Yang
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  9. Qixin Sun
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  10. Zhiyong Liu
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Corresponding authors

Correspondence to Qixin Sun or Zhiyong Liu.

Additional information

Communicated by B. Keller.

H. Zhang, H. Guan and J. Li contributed equally to this work.

The seed stock of 3D232 has been deposited to gene bank of CIMMYT, Beijing, China. Request seeds of 3D232 should address to Dr. Zhonghu He, CIMMYT Beijing at zhhecaas@gmail.com or directly to Dr. Zhiyong Liu, China Agricultural University at zhiyongliu@cau.edu.cn.

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Zhang, H., Guan, H., Li, J. et al. Genetic and comparative genomics mapping reveals that a powdery mildew resistance gene Ml3D232 originating from wild emmer co-segregates with an NBS-LRR analog in common wheat (Triticum aestivum L.). Theor Appl Genet 121, 1613–1621 (2010). https://doi.org/10.1007/s00122-010-1414-6

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