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Characterization and molecular mapping of Yr52 for high-temperature adult-plant resistance to stripe rust in spring wheat germplasm PI 183527

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Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most destructive diseases of wheat worldwide. Resistance is the best approach to control the disease. High-temperature adult-plant (HTAP) stripe rust resistance has proven to be race non-specific and durable. However, genes conferring high-levels of HTAP resistance are limited in number and new genes are urgently needed for breeding programs to develop cultivars with durable high-level resistance to stripe rust. Spring wheat germplasm PI 183527 showed a high-level of HTAP resistance against stripe rust in our germplasm evaluations over several years. To elucidate the genetic basis of resistance, we crossed PI 183527 and susceptible wheat line Avocet S. Adult plants of parents, F1, F2 and F2:3 progeny were tested with selected races under the controlled greenhouse conditions and in fields under natural infection. PI 183527 has a single dominant gene conferring HTAP resistance. Resistance gene analog polymorphism (RGAP) and simple sequence repeat (SSR) markers in combination with bulked segregant analysis (BSA) were used to identify markers linked to the resistance gene. A linkage map consisting of 4 RGAP and 7 SSR markers was constructed for the resistance gene using data from 175 F2 plants and their derived F2:3 lines. Amplification of nulli-tetrasomic, ditelosomic and deletion lines of Chinese Spring with three RGAP markers mapped the gene to the distal region (0.86–1.0) of chromosome 7BL. The molecular map spanned a genetic distance of 27.3 cM, and the resistance gene was narrowed to a 2.3-cM interval flanked by markers Xbarc182 and Xwgp5258. The polymorphism rates of the flanking markers in 74 wheat lines were 74 and 30 %, respectively; and the two markers in combination could distinguish the alleles at the resistance locus in 82 % of tested genotypes. To determine the genetic relationship between this resistance gene and Yr39, a gene also on 7BL conferring HTAP resistance in Alpowa, a cross was made between PI 183527 and Alpowa. F2 segregation indicated that the genes were 36.5 ± 6.75 cM apart. The gene in PI 183527 was therefore designed as Yr52. This new gene and flanking markers should be useful in developing wheat cultivars with high-level and possible durable resistance to stripe rust.

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Acknowledgments

This research was supported by the US Department of Agriculture, Agricultural Research Service (Project No. 5348-22000-014-00D), Washington Wheat Commission (Project No. 13C-3061-3925), and Vogel Foundation (Project No. 13Z-3061-3824). PPNS No. 0592, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center, Project Number WNP00663, Washington State University, Pullman, WA 99164-6430, USA. A scholarship from the China Scholarship Council to Runsheng Ren is gratefully acknowledged. We thank Drs. Robert McIntosh and Michael Pumphrey for critical review of the manuscript.

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  1. Department of Plant Pathology, China Agricultural University, Beijing, 100193, People’s Republic of China

    R. S. Ren & Z. J. Zhang

  2. Department of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA

    R. S. Ren, M. N. Wang & X. M. Chen

  3. US Department of Agriculture-Agricultural Research Service (USDA-ARS), Wheat Genetics, Quality, Physiology and Disease Research Unit, Pullman, WA, 99164-6430, USA

    X. M. Chen

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Correspondence to X. M. Chen.

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Communicated by T. Miedaner.

Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.

R. S. Ren and M. N. Wang made equal contributions.

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Ren, R.S., Wang, M.N., Chen, X.M. et al. Characterization and molecular mapping of Yr52 for high-temperature adult-plant resistance to stripe rust in spring wheat germplasm PI 183527. Theor Appl Genet 125, 847–857 (2012). https://doi.org/10.1007/s00122-012-1877-8

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