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High-temperature adult-plant (HTAP) stripe rust resistance gene Yr36 from Triticum turgidum ssp. dicoccoides is closely linked to the grain protein content locus Gpc-B1

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Abstract

Several new races of the stripe rust pathogen have become frequent throughout the wheat growing regions of the United States since 2000. These new races are virulent to most of the wheat seedling resistance genes limiting the resistance sources that can be used to combat this pathogen. High-temperature adult-plant (HTAP) stripe rust resistance has proven to be more durable than seedling resistance due to its non-race-specific nature, but its use is limited by the lack of mapping information. We report here the identification of a new HTAP resistance gene from Triticum turgidum ssp. dicoccoides (DIC) designated as Yr36. Lines carrying this gene were susceptible to almost all the stripe rust pathogen races tested at the seedling stage but showed adult-plant resistance to the prevalent races in California when tested at high diurnal temperatures. Isogenic lines for this gene were developed by six backcross generations. Field tests in two locations showed increased levels of field resistance to stripe rust and increased yields in isogenic lines carrying the Yr36 gene compared to those without the gene. Recombinant substitution lines of chromosome 6B from DIC in the isogenic background of durum cv. Langdon were used to map the Yr36 gene on the short arm of chromosome 6B completely linked to Xbarc101, and within a 2-cM interval defined by PCR-based markers Xucw71 and Xbarc136. Flanking locus Xucw71 is also closely linked to the grain protein content locus Gpc-B1 (0.3-cM). Marker-assisted selection strategies are presented to improve stripe rust resistance and simultaneously select for high or low Gpc-B1 alleles.

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Acknowledgements

The project was supported by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, grant number 2005-00975, USDA-IFAFS grant 2001-04462 and Research Grant Award No. US-3573-04C from BARD, The United States - Israel Binational Agricultural Research and Development Fund. The authors thank Drs. Kim Kidwell and Dipak Santra (Washington State University) for sharing unpublished mapping information.

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Authors and Affiliations

  1. Department of Plant Sciences, University of California, Davis, CA, 95616, USA

    Cristobal Uauy, Juan Carlos Brevis, Imtiaz Khan, Lee Jackson, Oswaldo Chicaiza & Jorge Dubcovsky

  2. USDA-ARS and Department of Plant Pathology, Washington State University, Pullman, WA, 99164, USA

    Xianming Chen

  3. The Institute of Evolution, University of Haifa, 31905, Mt. Carmel, Haifa, Israel

    Assaf Distelfeld & Tzion Fahima

Authors
  1. Cristobal Uauy
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  2. Juan Carlos Brevis
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  3. Xianming Chen
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  4. Imtiaz Khan
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  5. Lee Jackson
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  6. Oswaldo Chicaiza
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  7. Assaf Distelfeld
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  8. Tzion Fahima
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  9. Jorge Dubcovsky
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Corresponding author

Correspondence to Jorge Dubcovsky.

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Communicated by Beat Keller

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Uauy, C., Brevis, J.C., Chen, X. et al. High-temperature adult-plant (HTAP) stripe rust resistance gene Yr36 from Triticum turgidum ssp. dicoccoides is closely linked to the grain protein content locus Gpc-B1 . Theor Appl Genet 112, 97–105 (2005). https://doi.org/10.1007/s00122-005-0109-x

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