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A major QTL for durable leaf rust resistance widely exploited in durum wheat breeding programs maps on the distal region of chromosome arm 7BL

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Abstract

A recombinant inbred line (RIL) population and a set of advanced lines from multiple crosses were used to investigate the leaf rust (Puccinia triticina Eriks.) resistance carried by the durum wheat cultivar Creso and its derivatives (Colosseo and Plinio). One hundred seventy-six RILs from the cross Colosseo × Lloyd were tested under artificial rust inoculation in the field. The response at the seedling stage was also investigated. A major QTL (QLr.ubo-7B.2) for leaf rust resistance controlling both the seedling and the adult open field based-response was mapped on 7BL, with the favourable allele inherited from Colosseo. QLr.ubo-7B.2 showed R 2 and LOD peak values for the area under disease progress curve (AUDPC) equal to 72.9% and 44.5, respectively. The presence and location of QLr.ubo-7B.2 was validated by a linkage disequilibrium-based test using two-year field data of 62 advanced lines from 21 crosses with Creso, Colosseo or Plinio as resistance donors. QLr.ubo-7B.2 maps in a gene-dense region (7BL10-0.78-1.00) carrying several genes/QTLs in wheat and barley for resistance to rusts and other fungal diseases.

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Acknowledgments

Research carried out with the financial contribution of the European Union (BIOEXPLOIT project) and of the Emilia-Romagna Region (PRRIITT Project, Misura 3.4-A CEREALAB). The authors thank Sandra Stefanelli and Stefano Vecchi for technical assistance throughout the project.

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Authors and Affiliations

  1. Department of Agroenvironmental Science and Technology, University of Bologna, Viale G. Fanin 44, 40127, Bologna, Italy

    M. Maccaferri, P. Mantovani, R. Tuberosa, S. Giuliani & M. C. Sanguineti

  2. Società Produttori Sementi s.p.a., Via Macero 1, 40050, Argelato (BO), Italy

    E. DeAmbrogio, A. Demontis & A. Massi

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  1. M. Maccaferri
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  2. P. Mantovani
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Correspondence to M. C. Sanguineti.

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Communicated by J. Snape.

Electronic Supplementary Materials

122_2008_857_MOESM1_ESM.jpg

Supplemental Figure. Leaf rust reaction of Colosseo and Lloyd to the inoculation at the seedling stage with four different Puccinia triticina Italian isolates. The reaction to isolates 1, 9, 13 and 16 is reported in a, b, c and d, respectively (JPG 973 kb)

122_2008_857_MOESM2_ESM.xls

Supplemental data. Detailed haplotypes and field based-phenotypic data of the 62 advanced lines, originated from 21 crosses involving Creso or its resistant derivatives Colosseo and Plinio, and the susceptible checks. The haplotypes are based on the five SSRs mapped in the chr. 7BL region harboring QLr.ubo-7B.2 and significantly associated to leaf rust response. Mean values for leaf rust susceptibility index (LRS), i.e. the percentage of infected leaf area recorded at three different stages (early, medium and late) of the disease cycle, and for area under progressive disease curve (AUDPC) are reported. Phenotypic data were recorded in presence of articial inoculation in 2006 and 2007. The advanced lines have been listed according to their haplotypes (XLS 36 kb)

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Maccaferri, M., Mantovani, P., Tuberosa, R. et al. A major QTL for durable leaf rust resistance widely exploited in durum wheat breeding programs maps on the distal region of chromosome arm 7BL. Theor Appl Genet 117, 1225–1240 (2008). https://doi.org/10.1007/s00122-008-0857-5

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