Abstract
The utilization of lignocellulosic biomass as a petroleum alternative faces many challenges. This work reviews recent progress in the engineering of Escherichia coli and Klebsiella oxytoca to produce ethanol from biomass with minimal nutritional supplementation. A combination of directed engineering and metabolic evolution has resulted in microbial biocatalysts that produce up to 45 g L−1 ethanol in 48 h in a simple mineral salts medium, and convert various lignocellulosic materials to ethanol. Mutations contributing to ethanologenesis are discussed. The ethanologenic biocatalyst design approach was applied to other commodity chemicals, including optically pure d(−)- and l(+)-lactic acid, succinate and l-alanine with similar success. This review also describes recent progress in growth medium development, the reduction of hemicellulose hydrolysate toxicity and reduction of the demand for fungal cellulases.
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References
Administration EI (2005) US Department of Energy, Washington, DC, http://tonto.eia.doe.gov/FTPROOT/multifuel/038405.pdf
Administration EI (2007) US Department of Industry, Washington, DC, http://tonto.eia.doe.gov/FTPROOT/petroleum/psm/01090702.pdf
Raj KC, Talarico LA, Ingram LO, Maupin-Furlow JA (2002) Appl Environ Microbiol 68:2869
Lin Y, Tanaka S (2006) Appl Microbiol Biotechnol 69:627
Dien BS, Cotta MA, Jeffries TW (2003) Appl Microbiol Biotechnol 63:258
Zaldivar J, Nielsen J, Olsson L (2001) Appl Microbiol Biotechnol 56:17
Ingram LO, Aldrich HC, Borges ACC, Causey TB, Martinez A, Morales F, Saleh A, Underwood SA, Yomano LP, York SW, Zaldivar J, Zhou SD (1999) Biotechnol Prog 15:855
Ingram LO, Lai X, Moniruzzaman M, Wood BE, York SW (1997) Fuels and chemicals from biomass (ACS symposium series) 666:57
Hahnhagerdal B, Hallborn J, Jeppsson H, Olsson L, Skoog K, Walfridsson M (1993) In: Saddler JN (ed) Bioconversion of forest and agricultural plant residues. CAB International, Wallingford, UK, p 231
Feldmann SD, Sahm H, Sprenger GA (1992) Appl Microbiol Biotechnol 38:354
Lin ECC (1996) In: Neidhardt FC (ed) Escherichia coli and Salmonella: cellular and molecular biology, vol 1. ASM, Washington, DC, p 307
Grohmann K, Baldwin EA, Buslig BS, Ingram LO (1994) Biotechnol Lett 16:281
Alterthum F, Ingram LO (1989) Appl Environ Microbiol 55:1943
Ingram LO, Conway T (1988) Appl Environ Microbiol 54:397
Ingram LO, Conway T, Clark DP, Sewell GW, Preston JF (1987) Appl Environ Microbiol 53:2420
Neale AD, Scopes RK, Kelly JM (1988) Appl Microbiol Biotechnol 29:162
Ohta K, Beall DS, Mejia JP, Shanmugam KT, Ingram LO (1991) Appl Environ Microbiol 57:893
Yomano LP, York SW, Ingram LO (1998) J Ind Microbiol Biotechnol 20:132
Moniruzzaman M, Ingram LO (1998) Biotechnol Lett 20:943
Asghari A, Bothast RJ, Doran JB, Ingram LO (1996) J Indust Microbiol 16:42
Barbosa MDS, Beck MJ, Fein JE, Potts D, Ingram LO (1992) Appl Environ Microbiol 58:1382
Beall DS, Ingram LO, Benbassat A, Doran JB, Fowler DE, Hall RG, Wood BE (1992) Biotechnol Lett 14:857
Moniruzzaman M, Dien BS, Ferrer B, Hespell RB, Dale BE, Ingram LO, Bothast RJ (1996) Biotechnol Lett 18:985
Olsson L, Hahnhagerdal B, Zacchi G (1995) Biotechnol Bioeng 45:356
Doran JB, Cripe J, Sutton M, Foster B (2000) Appl Biochem Biotechnol 141:84–86
Leite AR, Guimaraes WV, de Araujo EF, Silva DO (2000) Braz J Microbiol 31:212
Rao K, Chaudhari V, Varanasi S, Kim DS (2007) Appl Microbiol Biotechnol 74:50
Jeoh T, Agblevor FA (2001) Biomass Bioenergy 21:109
Moniruzzaman M, York SW, Ingram LO (1998) J Ind Microbiol Biotechnol 20:281
Gonzalez R, Tao H, Shanmugam KT, York SW, Ingram LO (2002) Biotechnol Prog 18:6
York SW, Ingram LO (1996) Biotechnol Lett 18:683
Martinez A, York SW, Yomano LP, Pineda VL, Davis FC, Shelton JC, Ingram LO (1999) Biotechnol Prog 15:891
Underwood SA, Buszko ML, Shanmugam KT, Ingram LO (2002) Appl Environ Microbiol 68:1071
Underwood SA, Zhou S, Causey TB, Yomano LP, Shanmugam KT, Ingram LO (2002) Appl Environ Microbiol 68:6263
Lawford HG, Rousseau JD (1996) Appl Biochem Biotechnol 293:57–58
Dumsday GJ, Zhou B, Yaqin W, Stanley GA, Pamment NB (1999) J Ind Microbiol Biotechnol 23:701
Dumsday GJ, Zhou B, Buhmann S, Stanley GA, Pamment NB (1997) Australasian Biotechnol 7:300
Hasona A, York SW, Yomano LP, Ingram LO, Shanmugam KT (2002) Appl Environ Microbiol 68:2651
Zhou S, Yomano LP, Shanmugam KT, Ingram LO (2005) Biotechnol Lett 27:1891
Paul BJ, Ross W, Gaal T, Gourse RL (2004) Annu Rev Genet 38:749
Dennis PP, Ehrenberg M, Bremer H (2004) Microbiol Mol Biol Rev 68:639
Zhou S, Grabar TB, Shanmugam KT, Ingram LO (2006) Biotechnol Lett 28:671
Hespell RB, Wyckoff H, Dien BS, Bothast RJ (1996) Appl Environ Microbiol 62:4594
Dien BS, Nichols NN, O'Bryan PJ, Bothast RJ (2000) Appl Biochem Biotechnol 181:84–86
Martin GJO, Bin Zhou AK, Pamment NB (2006) J Ind Microbiol Biotechnol 33:834
Kim Y, Ingram LO, Shanmugam KT (2007) Appl Environ Microbiol 73:1766
Matjan F, Alam KY, Clark DP (1989) J Bacteriol 171:342
Cassey B, Guest JR, Attwood MM (1998) FEMS Microbiol Lett 159:325
de Graef MR, Alexeeva S, Snoep JL, de Mattos MJT (1999) J Bacteriol 181:2351
Lai XK, Davis FC, Hespell RB, Ingram LO (1997) Appl Environ Microbiol 63:355
Wood BE, Ingram LO (1992) Appl Environ Microbiol 58:2103
Ohta K, Beall DS, Mejia JP, Shanmugam KT, Ingram LO (1991) Appl Environ Microbiol 57:2810
Bothast RJ, Saha BC, Flosenzier AV, Ingram LO (1994) Biotechnol Lett 16:401
Brooks TA, Ingram LO (1995) Biotechnol Prog 11:619
Doran JB, Ingram LO (1993) Biotechnol Prog 9:533
Doran JB, Aldrich HC, Ingram LO (1994) Biotechnol Bioeng 44:240
Wood BE, Yomano LP, York SW, Ingram LO (2005) Biotechnol Prog 21:1366
Lowe SE, Zeikus JG (1992) J Gen Microbiol 138:803
Neale AD, Scopes RK, Wettenhall REH, Hoogenraad NJ (1987) J Bacteriol 169:1024
Bringermeyer S, Schimz KL, Sahm H (1986) Arch Microbiol 146:105
Hoppner TC, Doelle HW (1983) Eur J Appl Microbiol Biotechnol 17:152
Raj KC, Ingram LO, Maupin-Furlow JA (2001) Arch Microbiol 176:443
Talarico LA, Ingram LO, Maupin-Furlow JA (2001) Microbiology 147:2425
Nichols NN, Dien BS, Bothast RJ (2003) J Ind Microbiol Biotechnol 30:315
Gold RS, Meagher MM, Tong SX, Hutkins RW, Conway T (1996) Curr Microbiol 33:256
Hillman JD, Chen AP, Snoep JL (1996) Infect Immun 64:4319
Barbosa MDS, Ingram LO (1994) Curr Microbiol 28:279
Talarico LA, Gil MA, Yomano LP, Ingram LO, Maupin-Furlow JA (2005) Microbiology 151:4023
Liu SQ, Nichols NN, Dien BS, Cotta MA (2006) J Ind Microbiol Biotechnol 33:1
Inui M, Kawaguchi H, Murakami S, Vertes AA, Yukawa H (2004) J Mol Microbiol Biotechnol 8:243
Tao H, Gonzalez R, Martinez A, Rodriguez M, Ingram LO, Preston JF, Shanmugam KT (2001) J Bacteriol 183:2979
Gonzalez R, Tao H, Purvis JE, York SW, Shanmugam KT, Ingram LO (2003) Biotechnol Prog 19:612
Lynch AS, Lin ECC (1996) In: Neidhardt FC (ed) Escherichia coli and Salmonella: cellular and molecular biology, vol. 1. ASM, Washington, DC, p 1526
Lamark T, Rokenes TP, McDougall J, Strom AR (1996) J Bacteriol 178:1655
Compan I, Touati D (1994) Mol Microbiol 11:955
Martinez A, Grabar TB, Shanmugam KT, Yomano L, York SW, Ingram LO (2007) Biotechnol Lett 29:397
Causey TB, Zhou S, Shanmugam KT, Ingram LO (2003) Proc Natl Acad Sci USA 100:825
de Mattos MJT, Neijssel OM (1997) J Biotechnol 59:117
Teixeira de Mattos MJ, Neijssel OM (1997) J Biotechnol 59:117
Kempf B, Bremer E (1998) Arch Microbiol 170:319
Purvis JE, Yomano LP, Ingram LO (2005) Appl Environ Microbiol 71:3761
Miller EN, Ingram LO (2007) Biotechnol Lett 29:213
Gonzalez R, Tao H, Shanmugam KT, Ingram LO (2003) Abstr Pap Am Chem Soc 225:U200
Underwood SA, Buszko AL, Shanmugam KT, Ingram LO (2004) Appl Environ Microbiol 70:2734
Dupreez JC (1994) Enzyme Microb Technol 16:944
McMillan JD (1994) In: Himmel ME, Baker JO, Overend RP (eds) Enzymatic conversion of biomass for fuels production. American Chemical Society, Washington, DC, p 411
Klinke HB, Thomsen AB, Ahring BK (2004) Appl Microbiol Biotechnol 66:10
Grohmann K, Torget R, Himmel M (1985) Biotechnol Bioeng 15:59
Beall DS, Ohta K, Ingram LO (1991) Biotechnol Bioeng 38:296
Zaldivar J, Martinez A, Ingram LO (1999) Biotechnol Bioeng 65:24
Gutierrez T, Buszko ML, Ingram LO, Preston JF (2002) Appl Biochem Biotechnol 98:327
Gutierrez T, Ingram LO, Preston JF (2006) J Biotechnol 121:154
Zaldivar J, Ingram LO (1999) Biotechnol Bioeng 66:203
Zaldivar J, Martinez A, Ingram LO (2000) Biotechnol Bioeng 68:524
Martinez A, Rodriguez ME, York SW, Preston JF, Ingram LO (2000) Biotechnol Prog 16:637
Larsson S, Reimann A, Nilvebrant NO, Jonsson LJ (1999) Appl Biochem Biotechnol 91:77–79
Martinez A, Rodriguez ME, Wells ML, York SW, Preston JF, Ingram LO (2001) Biotechnol Prog 17:287
Heiner AP, Sugiyama J, Teleman O (1995) Carbohydr Res 273:207
Dowd MK, French AD, Reilly PJ (1992) Carbohydr Res 233:15
Ingram LO, Gomez PF, Lai X, Moniruzzaman M, Wood BE, Yomano LP, York SW (1998) Biotechnol Bioeng 58:204
Emert GH, Katzen R, Fredrickson RE, Kaupisch KF (1983) J Appl Polym Sci 37:787
Gauss WF, Suzuki S, Takagi M (1976) US Patent 3990944
Zhou SG, Ingram LO (2000) J Bacteriol 182:5676
Zhou SD, Yomano LP, Saleh AZ, Davis FC, Aldrich HC, Ingram LO (1999) Appl Environ Microbiol 65:2439
Zhou S, Ingram LO (1999) J Ind Microbiol Biotechnol 22:600
Zhou SD, Davis FC, Ingram LO (2001) Appl Environ Microbiol 67:6
Zhou SD, Ingram LO (2001) Biotechnol Lett 23:1455
Coughlan MP (1992) Bioresour Technol 39:107
Moniruzzaman M, Lai XK, York SW, Ingram LO (1997) Appl Environ Microbiol 63:4633
Wood BE, Aldrich HC, Ingram LO (1997) Biotechnol Prog 13:232
Agrawal AK, Bhalla R (2003) J Macromol Sci-Polym Rev C43:479
Lee SY, Hong SH, Lee SH, Park SJ (2004) Macromol Biosci 4:157
Ray SS, Bousmina M (2005) Prog Mater Sci 50:962
Wang SG, Cui WJ, Bei JZ (2005) Anal Bioanal Chem 381:547
Narayanan N, Roychoudhury PK, Srivastava A (2004) Electron J Biotechnol 7:167
Bianchi MM, Brambilla L, Protani F, Liu CL, Lievense J, Porro D (2001) Appl Environ Microbiol 67:5621
Porro D, Bianchi MM, Brambilla L, Menghini R, Bolzani D, Carrera V, Lievense J, Liu CL, Ranzi BM, Frontali L, Alberghina L (1999) Appl Environ Microbiol 65:4211
Saitoh S, Ishida N, Onishi T, Tokuhiro K, Nagamori E, Kitamoto K, Takahashi H (2005) Appl Environ Microbiol 71:2789
van Maris AJA, Winkler AA, Porro D, van Dijken JP, Pronk JT (2004) Appl Environ Microbiol 70:2898
Skory CD (2000) Appl Environ Microbiol 66:2343
Grabar TB, Zhou S, Shanmugam KT, Yomano L, Ingram LO (2006) Biotechnol Lett 28:1527
Chang DE, Jung HC, Rhee JS, Pan JG (1999) Appl Environ Microbiol 65:1384
Dien BS, Nichols NN, Bothast RJ (2001) J Ind Microbiol Biotechnol 27:259
Zhou SD, Causey TB, Hasona A, Shanmugam KT, Ingram LO (2003) Appl Environ Microbiol 69:399
Zhou S, Shanmugam KT, Yomano LP, Grabar TB, Ingram LO (2006) Biotechnol Lett 28:663
Zhu MM, Skraly FA, Cameron DC (2001) Metabolic Eng 3:218
Weber J, Kayser A, Rinas U (2005) Microbiology 151:707
Totemeyer S, Booth NA, Nichols WW, Dunbar B, Booth IR (1998) Mol Microbiol 27:553
Contiero J, Beatty C, Kumari S, DeSanti CL, Strohl WR, Wolfe A (2000) J Ind Microbiol Biotechnol 24:421
Varma A, Boesch BW, Palsson BO (1993) Appl Environ Microbiol 59:2465
Cheryan M, Parekh S, Shah M, Witjitra K (1997) Adv Appl Microbiol 43:1
Berraud C (2000) Biotechnol Lett 22:451
Freer SN (2002) World J Microbiol Biotechnol 18:271
Li Y, Chen J, Lun SY (2001) Appl Microbiol Biotechnol 57:451
Ondrey G (2001) Chem Eng 108:27
Yokota A, Terasawa Y, Takaoka N, Shimizu H, Tomita F (1994) Biosci Biotechnol Biochem 58:2164
Tomar A, Eiteman MA, Altman E (2003) Appl Microbiol Biotechnol 62:76
Causey TB, Shanmugam KT, Yomano LP, Ingram LO (2004) Proc Natl Acad Sci USA 101:2235
Werpy T, Petersen G (eds) (2004) Top value added chemicals from biomass, vol I. Pacific Northwest National Laboratory and National Renewable Energy Laboratory. Available at http://www.nrel.gov/docs/fy04osti/35523.pdf , last visited 3 July 2007
Parajo JC, Dominguez H, Dominguez JM (1998) Bioresour Technol 65:191
Nigam P, Singh D (1995) Process Biochem 30:117
Parajo JC, Dominguez H, Dominguez JM (1998) Bioresour Technol 65:203
Winkelhausen E, Kuzmanova S (1998) J Ferment Bioeng 86:1
Kim TB, Lee YJ, Kim P, Kim CS, Oh DK (2004) Biotechnol Lett 26:623
Kim TB, Oh DK (2003) Biotechnol Lett 25:2085
Cirino PC, Chin JW, Ingram LO (2006) Biotechnol Bioeng 95:1167
Lee SJ, Song H, Lee SY (2006) Appl Environ Microbiol 72:1939
Andersson C, Hodge D, Berglund KA, Rova U (2007) Biotechnol Prog 23:381
Buttke TM, Ingram LO (1980) Arch Biochem Biophys 203:565
Hols P, Kleerebezem M, Schanck AN, Ferain T, Hugenholtz J, Delcour J, de Vos WM (1999) Nat Biotechnol 17:588
Lee M, Smith GM, Eiteman MA, Altman E (2004) Appl Microbiol Biotechnol 65:56
Shibatani T, Kakimoto T, Chibata I (1979) Appl Environ Microbiol 38:359
Vonsivers M, Zacchi G, Olsson L, Hahnhagerdal B (1994) Biotechnol Prog 10:555
Lawford HG, Rousseau JD (1999) Appl Biochem Biotechnol 235:77–79
Zhang M, Eddy C, Deanda K, Finkestein M, Picataggio S (1995) Science 267:240
Jeon YJ, Svenson CJ, Rogers PL (2005) FEMS Microbiol Lett 244:85
Ho NWY, Chen ZD, Brainard AP (1998) Appl Environ Microbiol 64:1852
Sedlak M, Ho NWY (2004) Yeast 21:671
Kuyper M, Winkler AA, van Dijken JP, Pronk JT (2004) FEMS Yeast Res 4:655
Kuyper M, Toirkens MJ, Diderich JA, Winkler AA, van Dijken JP, Pronk JT (2005) FEMS Yeast Res 5:925
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Jarboe, L.R., Grabar, T.B., Yomano, L.P., Shanmugan, K.T., Ingram, L.O. (2007). Development of Ethanologenic Bacteria. In: Olsson, L. (eds) Biofuels. Advances in Biochemical Engineering/Biotechnology, vol 108. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2007_068
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